Product: Phospho-RSK3 (Thr356) Antibody
Catalog: AF8187
Description: Rabbit polyclonal antibody to Phospho-RSK3 (Thr356)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 83kDa; 83kD(Calculated).
Uniprot: Q15349
RRID: AB_2840249

View similar products>>

   Size Price Inventory
 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
Phospho-RSK3 (Thr356) Antibody detects endogenous levels of RSK3 only when phosphorylated at Thr356.
RRID:
AB_2840249
Cite Format: Affinity Biosciences Cat# AF8187, RRID:AB_2840249.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

90 kDa ribosomal protein S6 kinase 2; HU 2; KS6A2_HUMAN; MAP kinase activated protein kinase 1c; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK-1c; MAPKAPK1C; Mitogen.activated protein kinase-activated protein kinase 1C; p90 RSK3; p90-RSK 2; p90RSK2; pp90RSK3; Ribosomal protein S6 kinase alpha-2; ribosomal protein S6 kinase, 90kDa, polypeptide 2; Ribosomal S6 kinase 3; RPS6KA2; RSK 3; RSK; RSK-3; S6K alpha; S6K alpha 2; S6K-alpha-2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q15349 KS6A2_HUMAN:

Widely expressed with higher expression in lung, skeletal muscle, brain, uterus, ovary, thyroid and prostate.

Sequence:
MDLSMKKFAVRRFFSVYLRRKSRSKSSSLSRLEEEGVVKEIDISHHVKEGFEKADPSQFELLKVLGQGSYGKVFLVRKVKGSDAGQLYAMKVLKKATLKVRDRVRSKMERDILAEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALALDHLHSLGIIYRDLKPENILLDEEGHIKITDFGLSKEAIDHDKRAYSFCGTIEYMAPEVVNRRGHTQSADWWSFGVLMFEMLTGSLPFQGKDRKETMALILKAKLGMPQFLSGEAQSLLRALFKRNPCNRLGAGIDGVEEIKRHPFFVTIDWNTLYRKEIKPPFKPAVGRPEDTFHFDPEFTARTPTDSPGVPPSANAHHLFRGFSFVASSLIQEPSQQDLHKVPVHPIVQQLHGNNIHFTDGYEIKEDIGVGSYSVCKRCVHKATDTEYAVKIIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKFVYLVMELMRGGELLDRILRQRYFSEREASDVLCTITKTMDYLHSQGVVHRDLKPSNILYRDESGSPESIRVCDFGFAKQLRAGNGLLMTPCYTANFVAPEVLKRQGYDAACDIWSLGILLYTMLAGFTPFANGPDDTPEEILARIGSGKYALSGGNWDSISDAAKDVVSKMLHVDPHQRLTAMQVLKHPWVVNREYLSPNQLSRQDVHLVKGAMAATYFALNRTPQAPRLEPVLSSNLAQRRGMKRLTSTRL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
91
Zebrafish
91
Chicken
91
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15349 As Substrate

Site PTM Type Enzyme
S26 Phosphorylation
S27 Phosphorylation
S28 Phosphorylation
S30 Phosphorylation
K91 Acetylation
K94 Acetylation
S106 Phosphorylation
Y135 Phosphorylation
K186 Ubiquitination
S206 Phosphorylation
Y217 Phosphorylation
S218 Phosphorylation O15530 (PDPK1) , Q15349 (RPS6KA2)
T222 Phosphorylation
K295 Acetylation
T356 Phosphorylation
S360 Phosphorylation
S377 Phosphorylation
S381 Phosphorylation
S388 Phosphorylation
Y426 Phosphorylation
K430 Ubiquitination
K450 Ubiquitination
Y463 Phosphorylation
T471 Phosphorylation
K473 Ubiquitination
S546 Phosphorylation
T570 Phosphorylation Q15349 (RPS6KA2)
Y573 Phosphorylation
T574 Phosphorylation
T618 Phosphorylation
S628 Phosphorylation
K630 Ubiquitination
S650 Phosphorylation
T662 Phosphorylation
S730 Phosphorylation

PTMs - Q15349 As Enzyme

Substrate Site Source
P16220 (CREB1) S133 Uniprot
P17676 (CEBPB) S321 Uniprot
P25963 (NFKBIA) S32 Uniprot
P32004-2 (L1CAM) S1152 Uniprot
Q15349-1 (RPS6KA2) S218 Uniprot
Q15349-1 (RPS6KA2) T222 Uniprot
Q15349 (RPS6KA2) T570 Uniprot
Q92934 (BAD) S75 Uniprot
Q92934 (BAD) S118 Uniprot

Research Backgrounds

Function:

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells.

PTMs:

Activated by phosphorylation at Ser-218 by PDPK1. Autophosphorylated on Ser-377, as part of the activation process. May be phosphorylated at Thr-356 and Ser-360 by MAPK1/ERK2 and MAPK3/ERK1 (By similarity).

N-terminal myristoylation results in an activated kinase in the absence of added growth factors.

Subcellular Location:

Nucleus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed with higher expression in lung, skeletal muscle, brain, uterus, ovary, thyroid and prostate.

Subunit Structure:

Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells. Transiently dissociates following mitogenic stimulation (By similarity). Interacts with FBXO5; cooperate to induce the metaphase arrest of early blastomeres; increases and stabilizes interaction of FBXO5 with CDC20 (By similarity).

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

References

1). DHA-enriched phosphatidylcholine from Clupea harengus roes regulates the gut–liver axis to ameliorate high-fat diet-induced non-alcoholic fatty liver disease. Food & Function, 2022 (PubMed: 36263717) [IF=6.1]

2). Laminaria japonica fucoidan ameliorates cyclophosphamide‐induced liver and kidney injury possibly by regulating Nrf2/HO‐1 and TLR4/NF‐κB signaling pathways. Journal of the Science of Food and Agriculture, 2022 (PubMed: 34689333) [IF=4.1]

3). High concentrate diet induced inflammatory response and tight junction disruption in the mammary gland of dairy cows. Microbial Pathogenesis, 2023 (PubMed: 36709006) [IF=3.8]

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.