Phospho-SIK1(T182)/SIK2(T175)/SIK3(T221) Antibody - #AF8185
| Product: | Phospho-SIK1(T182)/SIK2(T175)/SIK3(T221) Antibody |
| Catalog: | AF8185 |
| Description: | Rabbit polyclonal antibody to Phospho-SIK1(T182)/SIK2(T175)/SIK3(T221) |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Dog, Chicken, Xenopus |
| Mol.Wt.: | 140KD; 85kD,104kD,145kD(Calculated). |
| Uniprot: | P57059 | Q9H0K1 | Q9Y2K2 |
| RRID: | AB_2840247 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF8185, RRID:AB_2840247.
Fold/Unfold
KID2; MSK; myocardial SNF1 like kinase; QIK; Qin-induced kinase; Salt inducible kinase 1; Salt-inducible protein kinase 1; Serine threonine protein kinase SNF1 like kinase 1; Serine threonine protein kinase SNF1LK; Serine/threonine-protein kinase SIK1; Serine/threonine-protein kinase SNF1-like kinase 1; Serine/threonine-protein kinase SNF1LK; SIK-1; SIK1; SIK1_HUMAN; SNF1 like kinase; DKFZp434K1115; EC 2.7.11.1; KIAA0781; LOH11CR1I; QIK; Qin induced kinase; Qin-induced kinase; Salt inducible kinase 2; Salt inducible protein kinase 2; Salt inducible serine/threonine kinase 2; Salt-inducible protein kinase 2; Serine/threonine protein kinase SIK2; Serine/threonine protein kinase SNF1 like kinase 2; Serine/threonine-protein kinase SIK2; Serine/threonine-protein kinase SNF1-like kinase 2; SIK 2; SIK-2; SIK2; SIK2_HUMAN; SNF1 like kinase; SNF1 like kinase 2; SNF1LK2; FLJ12240; KIAA0999; L19; QSK; Salt inducible kinase 3; serine/threonine-protein kinase QSK; Serine/threonine-protein kinase SIK3; SIK 3; SIK family kinase 3; SIK3_HUMAN;
Immunogens
- P57059 SIK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVIMSEFSADPAGQGQGQQKPLRVGFYDIERTLGKGNFAVVKLARHRVTKTQVAIKIIDKTRLDSSNLEKIYREVQLMKLLNHPHIIKLYQVMETKDMLYIVTEFAKNGEMFDYLTSNGHLSENEARKKFWQILSAVEYCHDHHIVHRDLKTENLLLDGNMDIKLADFGFGNFYKSGEPLSTWCGSPPYAAPEVFEGKEYEGPQLDIWSLGVVLYVLVCGSLPFDGPNLPTLRQRVLEGRFRIPFFMSQDCESLIRRMLVVDPARRITIAQIRQHRWMRAEPCLPGPACPAFSAHSYTSNLGDYDEQALGIMQTLGVDRQRTVESLQNSSYNHFAAIYYLLLERLKEYRNAQCARPGPARQPRPRSSDLSGLEVPQEGLSTDPFRPALLCPQPQTLVQSVLQAEMDCELQSSLQWPLFFPVDASCSGVFRPRPVSPSSLLDTAISEEARQGPGLEEEQDTQESLPSSTGRRHTLAEVSTRLSPLTAPCIVVSPSTTASPAEGTSSDSCLTFSASKSPAGLSGTPATQGLLGACSPVRLASPFLGSQSATPVLQAQGGLGGAVLLPVSFQEGRRASDTSLTQGLKAFRQQLRKTTRTKGFLGLNKIKGLARQVCQAPASRASRGGLSPFHAPAQSPGLHGGAAGSREGWSLLEEVLEQQRLLQLQHHPAAAPGCSQAPQPAPAPFVIAPCDGPGAAPLPSTLLTSGLPLLPPPLLQTGASPVASAAQLLDTHLHIGTGPTALPAVPPPRLARLAPGCEPLGLLQGDCEMEDLMPCSLGTFVLVQ
- Q9H0K1 SIK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVMADGPRHLQRGPVRVGFYDIEGTLGKGNFAVVKLGRHRITKTEVAIKIIDKSQLDAVNLEKIYREVQIMKMLDHPHIIKLYQVMETKSMLYLVTEYAKNGEIFDYLANHGRLNESEARRKFWQILSAVDYCHGRKIVHRDLKAENLLLDNNMNIKIADFGFGNFFKSGELLATWCGSPPYAAPEVFEGQQYEGPQLDIWSMGVVLYVLVCGALPFDGPTLPILRQRVLEGRFRIPYFMSEDCEHLIRRMLVLDPSKRLTIAQIKEHKWMLIEVPVQRPVLYPQEQENEPSIGEFNEQVLRLMHSLGIDQQKTIESLQNKSYNHFAAIYFLLVERLKSHRSSFPVEQRLDGRQRRPSTIAEQTVAKAQTVGLPVTMHSPNMRLLRSALLPQASNVEAFSFPASGCQAEAAFMEEECVDTPKVNGCLLDPVPPVLVRKGCQSLPSNMMETSIDEGLETEGEAEEDPAHAFEAFQSTRSGQRRHTLSEVTNQLVVMPGAGKIFSMNDSPSLDSVDSEYDMGSVQRDLNFLEDNPSLKDIMLANQPSPRMTSPFISLRPTNPAMQALSSQKREVHNRSPVSFREGRRASDTSLTQGIVAFRQHLQNLARTKGILELNKVQLLYEQIGPEADPNLAPAAPQLQDLASSCPQEEVSQQQESVSTLPASVHPQLSPRQSLETQYLQHRLQKPSLLSKAQNTCQLYCKEPPRSLEQQLQEHRLQQKRLFLQKQSQLQAYFNQMQIAESSYPQPSQQLPLPRQETPPPSQQAPPFSLTQPLSPVLEPSSEQMQYSPFLSQYQEMQLQPLPSTSGPRAAPPLPTQLQQQQPPPPPPPPPPRQPGAAPAPLQFSYQTCELPSAASPAPDYPTPCQYPVDGAQQSDLTGPDCPRSPGLQEAPSSYDPLALSELPGLFDCEMLDAVDPQHNGYVLVN
- Q9Y2K2 SIK3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAAASGAGGAAGAGTGGAGPAGRLLPPPAPGSPAAPAAVSPAAGQPRPPAPASRGPMPARIGYYEIDRTIGKGNFAVVKRATHLVTKAKVAIKIIDKTQLDEENLKKIFREVQIMKMLCHPHIIRLYQVMETERMIYLVTEYASGGEIFDHLVAHGRMAEKEARRKFKQIVTAVYFCHCRNIVHRDLKAENLLLDANLNIKIADFGFSNLFTPGQLLKTWCGSPPYAAPELFEGKEYDGPKVDIWSLGVVLYVLVCGALPFDGSTLQNLRARVLSGKFRIPFFMSTECEHLIRHMLVLDPNKRLSMEQICKHKWMKLGDADPNFDRLIAECQQLKEERQVDPLNEDVLLAMEDMGLDKEQTLQSLRSDAYDHYSAIYSLLCDRHKRHKTLRLGALPSMPRALAFQAPVNIQAEQAGTAMNISVPQVQLINPENQIVEPDGTLNLDSDEGEEPSPEALVRYLSMRRHTVGVADPRTEVMEDLQKLLPGFPGVNPQAPFLQVAPNVNFMHNLLPMQNLQPTGQLEYKEQSLLQPPTLQLLNGMGPLGRRASDGGANIQLHAQQLLKRPRGPSPLVTMTPAVPAVTPVDEESSDGEPDQEAVQSSTYKDSNTLHLPTERFSPVRRFSDGAASIQAFKAHLEKMGNNSSIKQLQQECEQLQKMYGGQIDERTLEKTQQQHMLYQQEQHHQILQQQIQDSICPPQPSPPLQAACENQPALLTHQLQRLRIQPSSPPPNHPNNHLFRQPSNSPPPMSSAMIQPHGAASSSQFQGLPSRSAIFQQQPENCSSPPNVALTCLGMQQPAQSQQVTIQVQEPVDMLSNMPGTAAGSSGRGISISPSAGQMQMQHRTNLMATLSYGHRPLSKQLSADSAEAHSLNVNRFSPANYDQAHLHPHLFSDQSRGSPSSYSPSTGVGFSPTQALKVPPLDQFPTFPPSAHQQPPHYTTSALQQALLSPTPPDYTRHQQVPHILQGLLSPRHSLTGHSDIRLPPTEFAQLIKRQQQQRQQQQQQQQQQEYQELFRHMNQGDAGSLAPSLGGQSMTERQALSYQNADSYHHHTSPQHLLQIRAQECVSQASSPTPPHGYAHQPALMHSESMEEDCSCEGAKDGFQDSKSSSTLTKGCHDSPLLLSTGGPGDPESLLGTVSHAQELGIHPYGHQPTAAFSKNKVPSREPVIGNCMDRSSPGQAVELPDHNGLGYPARPSVHEHHRPRALQRHHTIQNSDDAYVQLDNLPGMSLVAGKALSSARMSDAVLSQSSLMGSQQFQDGENEECGASLGGHEHPDLSDGSQHLNSSCYPSTCITDILLSYKHPEVSFSMEQAGV
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P57059/Q9H0K1/Q9Y2K2 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K20 | Sumoylation | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| T32 | Phosphorylation | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K175 | Sumoylation | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| T182 | Phosphorylation | Q15831 (STK11) , P49841 (GSK3B) | Uniprot |
| S186 | Phosphorylation | P57059 (SIK1) | Uniprot |
| T322 | Phosphorylation | Q14012 (CAMK1) | Uniprot |
| S370 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| T460 | Phosphorylation | Uniprot | |
| T473 | Phosphorylation | Uniprot | |
| T479 | Phosphorylation | Uniprot | |
| S516 | Phosphorylation | Uniprot | |
| S534 | Phosphorylation | Uniprot | |
| S575 | Phosphorylation | Uniprot | |
| T577 | Phosphorylation | Uniprot | |
| K584 | Sumoylation | Uniprot | |
| K584 | Ubiquitination | Uniprot | |
| K597 | Ubiquitination | Uniprot | |
| K604 | Sumoylation | Uniprot | |
| K604 | Ubiquitination | Uniprot | |
| S626 | Phosphorylation | Uniprot | |
| S634 | Phosphorylation | Uniprot | |
| S644 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T25 | Phosphorylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K49 | Ubiquitination | Uniprot | |
| K53 | Acetylation | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| K168 | Sumoylation | Uniprot | |
| T175 | Phosphorylation | Q15831 (STK11) | Uniprot |
| K266 | Ubiquitination | Uniprot | |
| K313 | Ubiquitination | Uniprot | |
| S342 | Phosphorylation | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | P31751 (AKT2) , P17612 (PRKACA) | Uniprot |
| T359 | Phosphorylation | Uniprot | |
| S379 | Phosphorylation | Uniprot | |
| T484 | Phosphorylation | Uniprot | |
| S486 | Phosphorylation | Uniprot | |
| S512 | Phosphorylation | Uniprot | |
| S534 | Phosphorylation | Uniprot | |
| K536 | Ubiquitination | Uniprot | |
| S545 | Phosphorylation | Uniprot | |
| R547 | Methylation | Uniprot | |
| S576 | Phosphorylation | Uniprot | |
| S587 | Phosphorylation | Uniprot | |
| T589 | Phosphorylation | Uniprot | |
| S590 | Phosphorylation | Uniprot | |
| K686 | Ubiquitination | Uniprot | |
| K692 | Ubiquitination | Uniprot | |
| Y700 | Phosphorylation | Uniprot | |
| K702 | Ubiquitination | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T71 | Phosphorylation | Uniprot | |
| T221 | Phosphorylation | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| T469 | Phosphorylation | Uniprot | |
| S551 | Phosphorylation | Uniprot | |
| S626 | Phosphorylation | Uniprot | |
| S866 | Phosphorylation | Uniprot | |
| S978 | Phosphorylation | Uniprot | |
| S1111 | Phosphorylation | Uniprot | |
| T1159 | Phosphorylation | Uniprot |
PTMs - P57059/Q9H0K1/Q9Y2K2 As Enzyme
| Substrate | Site | Source |
|---|---|---|
| P04637 (TP53) | S15 | Uniprot |
| P10636 (MAPT) | T529 | Uniprot |
| P10636 (MAPT) | S531 | Uniprot |
| P10636 (MAPT) | S579 | Uniprot |
| P10636 (MAPT) | S610 | Uniprot |
| P10636 (MAPT) | S622 | Uniprot |
| P10636 (MAPT) | S641 | Uniprot |
| P10636 (MAPT) | S673 | Uniprot |
| P36956 (SREBF1) | S337 | Uniprot |
| P36956 (SREBF1) | S338 | Uniprot |
| P36956 (SREBF1) | S402 | Uniprot |
| P56524 (HDAC4) | S246 | Uniprot |
| P56524 (HDAC4) | S467 | Uniprot |
| P57059 (SIK1) | S186 | Uniprot |
| Q53ET0 (CRTC2) | S70 | Uniprot |
| Q53ET0 (CRTC2) | S171 | Uniprot |
| Q53ET0 (CRTC2) | S274 | Uniprot |
| Q53ET0 (CRTC2) | S306 | Uniprot |
| Q53ET0 (CRTC2) | S348 | Uniprot |
| Q6UUV7 (CRTC3) | S62 | Uniprot |
| Q6UUV7 (CRTC3) | S162 | Uniprot |
| Q6UUV7 (CRTC3) | S329 | Uniprot |
| Q6UUV7 (CRTC3) | S370 | Uniprot |
| Q6UUV9 (CRTC1) | S151 | Uniprot |
| Q6UUV9 (CRTC1) | S167 | Uniprot |
| Q9UQL6 (HDAC5) | S259 | Uniprot |
| Q9UQL6 (HDAC5) | S498 | Uniprot |
Research Backgrounds
Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, gluconeogenesis and lipogenesis regulation, muscle growth and differentiation and tumor suppression. Phosphorylates HDAC4, HDAC5, PPME1, SREBF1, CRTC1/TORC1. Inhibits CREB activity by phosphorylating and inhibiting activity of TORCs, the CREB-specific coactivators, like CRTC2/TORC2 and CRTC3/TORC3 in response to cAMP signaling. Acts as a tumor suppressor and plays a key role in p53/TP53-dependent anoikis, a type of apoptosis triggered by cell detachment: required for phosphorylation of p53/TP53 in response to loss of adhesion and is able to suppress metastasis. Part of a sodium-sensing signaling network, probably by mediating phosphorylation of PPME1: following increases in intracellular sodium, SIK1 is activated by CaMK1 and phosphorylates PPME1 subunit of protein phosphatase 2A (PP2A), leading to dephosphorylation of sodium/potassium-transporting ATPase ATP1A1 and subsequent increase activity of ATP1A1. Acts as a regulator of muscle cells by phosphorylating and inhibiting class II histone deacetylases HDAC4 and HDAC5, leading to promote expression of MEF2 target genes in myocytes. Also required during cardiomyogenesis by regulating the exit of cardiomyoblasts from the cell cycle via down-regulation of CDKN1C/p57Kip2. Acts as a regulator of hepatic gluconeogenesis by phosphorylating and repressing the CREB-specific coactivators CRTC1/TORC1 and CRTC2/TORC2, leading to inhibit CREB activity. Also regulates hepatic lipogenesis by phosphorylating and inhibiting SREBF1. In concert with CRTC1/TORC1, regulates the light-induced entrainment of the circadian clock by attenuating PER1 induction; represses CREB-mediated transcription of PER1 by phosphorylating and deactivating CRTC1/TORC1 (By similarity).
Phosphorylated at Thr-182 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39, leading to its activation. Phosphorylation at Thr-182 promotes autophosphorylation at Ser-186, which is required for sustained activity. Autophosphorylation at Ser-186 is maintained by sequential phosphorylation at Thr-182 by GSK3-beta. GSK3-beta cannot initiate phosphorylation at Thr-182, it can only maintain it. Phosphorylation at Ser-575 in response to cAMP signaling promotes translocation to the cytoplasm. Phosphorylation at Thr-322 by CaMK1 following intracellular sodium concentration leads to activation.
Cytoplasm. Nucleus.
Note: Locates to cytoplasm when inactive following cAMP-induced phosphorylation, probably by PKA (PubMed:29211348).
Interacts with ATP1A1 (By similarity). Interacts (when phosphorylated on Thr-182 and Ser-186) with YWHAZ. Interacts (when phosphorylated at Thr-473 and/or Ser-575) with 14-3-3 proteins; the interaction inhibits kinase activity towards TORCs. There is a cooperative effect of the phosphorylation sites in 14-3-3 binding as the interaction is stronger when both Thr-473 and Ser-575 are modified.
The RK-rich region determines the subcellular location and is required for cAMP responsiveness.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily.
Phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators.
Phosphorylated at Thr-175 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39.
Acetylation at Lys-53 inhibits kinase activity. Deacetylated by HDAC6.
Cytoplasm.
Interacts with and phosphorylates TORC2/CRTC2.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Positive regulator of mTOR signaling that functions by triggering the degradation of DEPTOR, an mTOR inhibitor. Involved in the dynamic regulation of mTOR signaling in chondrocyte differentiation during skeletogenesis. Negatively regulates cAMP signaling pathway possibly by acting on CRTC2/TORC2 and CRTC3/TORC3 (Probable). Prevents HDAC4 translocation to the nucleus (By similarity).
Phosphorylated at Thr-221 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-221 is inhibited in response to PTHLH/PTHrP. Phosphorylated at Thr-469 and Ser-551 in response to cAMP signaling (Probable).
Cytoplasm.
Note: Locates to punctate structures within the cytoplasm on binding to YWHAZ.
Expressed in chondrocytes.
Binds to and is activated by YWHAZ when phosphorylated on Thr-221. Interacts with 14-3-3 proteins. Interacts with HDAC4; this interaction leads to HDAC4 retention in the cytoplasm (By similarity). Interacts with DEPTOR, MLST8/GbetaL, RICTOR and RPTOR.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Research Fields
· Organismal Systems > Endocrine system > Glucagon signaling pathway.
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