mGluR7 Antibody | Affinity Biosciences

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Western blot analysis of extracts from 3T3, using mGluR7 Antibody.

AF0171 at 1/100 staining human brain tissue sections by IHC-P.

Product:	mGluR7 Antibody
Catalog:	AF0171
Description:	Rabbit polyclonal antibody to mGluR7
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Dog
Mol.Wt.:	100kDa, 80 kDa; 102kD(Calculated).
Uniprot:	Q14831
RRID:	AB_2833364

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%), Horse(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

mGluR7 Antibody detects endogenous levels of total mGluR7.

RRID:

AB_2833364
Cite Format: Affinity Biosciences Cat# AF0171, RRID:AB_2833364.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ40498; GLUR7; Glutamate receptor metabotropic 7; GPRC1G; GRM7; GRM7_HUMAN; Metabotropic glutamate receptor 7; MGLU7; mGluR7; OTTHUMP00000206961; OTTHUMP00000214674; OTTHUMP00000214675;

Immunogens

Immunogen:

Uniprot:

Q14831(GRM7_HUMAN) >>Visit HPA database.

Gene(ID):

GRM7(2917)

Expression:

Q14831 GRM7_HUMAN:

Expressed in many areas of the brain, especially in the cerebral cortex, hippocampus, and cerebellum. Expression of GRM7 isoforms in non-neuronal tissues appears to be restricted to isoform 3 and isoform 4.

Description:

mGluR7 Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. Belongs to the G-protein coupled receptor 3 family. Interacts with PICK1. 5 isoforms of the human protein are produced by alternative splicing.

Sequence:

Q14831 GRM7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVQLRKLLRVLTLMKFPCCVLEVLLCALAAAARGQEMYAPHSIRIEGDVTLGGLFPVHAKGPSGVPCGDIKRENGIHRLEAMLYALDQINSDPNLLPNVTLGARILDTCSRDTYALEQSLTFVQALIQKDTSDVRCTNGEPPVFVKPEKVVGVIGASGSSVSIMVANILRLFQIPQISYASTAPELSDDRRYDFFSRVVPPDSFQAQAMVDIVKALGWNYVSTLASEGSYGEKGVESFTQISKEAGGLCIAQSVRIPQERKDRTIDFDRIIKQLLDTPNSRAVVIFANDEDIKQILAAAKRADQVGHFLWVGSDSWGSKINPLHQHEDIAEGAITIQPKRATVEGFDAYFTSRTLENNRRNVWFAEYWEENFNCKLTISGSKKEDTDRKCTGQERIGKDSNYEQEGKVQFVIDAVYAMAHALHHMNKDLCADYRGVCPEMEQAGGKKLLKYIRNVNFNGSAGTPVMFNKNGDAPGRYDIFQYQTTNTSNPGYRLIGQWTDELQLNIEDMQWGKGVREIPASVCTLPCKPGQRKKTQKGTPCCWTCEPCDGYQYQFDEMTCQHCPYDQRPNENRTGCQDIPIIKLEWHSPWAVIPVFLAMLGIIATIFVMATFIRYNDTPIVRASGRELSYVLLTGIFLCYIITFLMIAKPDVAVCSFRRVFLGLGMCISYAALLTKTNRIYRIFEQGKKSVTAPRLISPTSQLAITSSLISVQLLGVFIWFGVDPPNIIIDYDEHKTMNPEQARGVLKCDITDLQIICSLGYSILLMVTCTVYAIKTRGVPENFNEAKPIGFTMYTTCIVWLAFIPIFFGTAQSAEKLYIQTTTLTISMNLSASVALGMLYMPKVYIIIFHPELNVQKRKRSFKAVVTAATMSSRLSHKPSDRPNGEAKTELCENVDPNSPAAKKKYVSYNNLVI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Dog

100

Pig

Sheep

Chicken

Rabbit

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14831 As Substrate

Q14831

Site	PTM Type	Source
T12	Phosphorylation	Uniprot
T223	Phosphorylation	Uniprot
S226	Phosphorylation	Uniprot
S229	Phosphorylation	Uniprot
T264	Phosphorylation	Uniprot
T675	Phosphorylation	Uniprot
T677	Phosphorylation	Uniprot
T822	Phosphorylation	Uniprot
S862	Phosphorylation	Uniprot
S874	Phosphorylation	Uniprot
K889	Sumoylation	Uniprot

Research Backgrounds

Q14831_GRM7_HUMAN

Function:

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Subunit Structure:

Interacts with PICK1.

Family&Domains:

Belongs to the G-protein coupled receptor 3 family.

Research Fields

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Nervous system > Glutamatergic synapse.

References

1). Therapeutic potential of alkaloid extract from Codonopsis Radix in alleviating hepatic lipid accumulation: insights into mitochondrial energy metabolism and endoplasmic reticulum stress regulation in NAFLD mice. Chinese Journal of Natural Medicines (PubMed: 37407172) [IF=4.6]

2). FAdV-4 induce autophagy via the endoplasmic reticulum stress-related unfolded protein response. Veterinary Microbiology (PubMed: 35487017) [IF=3.3]

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mGluR7 Antibody - #AF0171