Product: Phospho-Flt3 / CD135 (Tyr591) Antibody
Catalog: AF8152
Description: Rabbit polyclonal antibody to Phospho-Flt3 / CD135 (Tyr591)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 113kDa; 113kD(Calculated).
Uniprot: P36888
RRID: AB_2840214

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 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(82%), Bovine(82%), Horse(91%), Sheep(100%), Rabbit(91%), Dog(91%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
Phospho-Flt3 / CD135 (Tyr591) Antibody detects endogenous levels of Flt3 / CD135 only when phosphorylated at Tyr591.
RRID:
AB_2840214
Cite Format: Affinity Biosciences Cat# AF8152, RRID:AB_2840214.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD 135; CD135; CD135 antigen; Fetal liver kinase 2; FL cytokine receptor; Flk 2; Flk2; Flt 3; FLT-3; Flt3; FLT3_HUMAN; FMS like tyrosine kinase 3; Fms related tyrosine kinase 3; Fms-like tyrosine kinase 3; Growth factor receptor tyrosine kinase type III; Ly-72; OTTHUMP0000004234; Receptor type tyrosine protein kinase FLT3; Stem cell tyrosine kinase 1; Stk 1; STK-1; Stk1; Tyrosine protein kinase receptor FLT3; Tyrosine-protein kinase receptor FLT3;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P36888 FLT3_HUMAN:

Detected in bone marrow, in hematopoietic stem cells, in myeloid progenitor cells and in granulocyte/macrophage progenitor cells (at protein level). Detected in bone marrow, liver, thymus, spleen and lymph node, and at low levels in kidney and pancreas. Highly expressed in T-cell leukemia.

Sequence:
MPALARDGGQLPLLVVFSAMIFGTITNQDLPVIKCVLINHKNNDSSVGKSSSYPMVSESPEDLGCALRPQSSGTVYEAAAVEVDVSASITLQVLVDAPGNISCLWVFKHSSLNCQPHFDLQNRGVVSMVILKMTETQAGEYLLFIQSEATNYTILFTVSIRNTLLYTLRRPYFRKMENQDALVCISESVPEPIVEWVLCDSQGESCKEESPAVVKKEEKVLHELFGTDIRCCARNELGRECTRLFTIDLNQTPQTTLPQLFLKVGEPLWIRCKAVHVNHGFGLTWELENKALEEGNYFEMSTYSTNRTMIRILFAFVSSVARNDTGYYTCSSSKHPSQSALVTIVEKGFINATNSSEDYEIDQYEEFCFSVRFKAYPQIRCTWTFSRKSFPCEQKGLDNGYSISKFCNHKHQPGEYIFHAENDDAQFTKMFTLNIRRKPQVLAEASASQASCFSDGYPLPSWTWKKCSDKSPNCTEEITEGVWNRKANRKVFGQWVSSSTLNMSEAIKGFLVKCCAYNSLGTSCETILLNSPGPFPFIQDNISFYATIGVCLLFIVVLTLLICHKYKKQFRYESQLQMVQVTGSSDNEYFYVDFREYEYDLKWEFPRENLEFGKVLGSGAFGKVMNATAYGISKTGVSIQVAVKMLKEKADSSEREALMSELKMMTQLGSHENIVNLLGACTLSGPIYLIFEYCCYGDLLNYLRSKREKFHRTWTEIFKEHNFSFYPTFQSHPNSSMPGSREVQIHPDSDQISGLHGNSFHSEDEIEYENQKRLEEEEDLNVLTFEDLLCFAYQVAKGMEFLEFKSCVHRDLAARNVLVTHGKVVKICDFGLARDIMSDSNYVVRGNARLPVKWMAPESLFEGIYTIKSDVWSYGILLWEIFSLGVNPYPGIPVDANFYKLIQNGFKMDQPFYATEEIYIIMQSCWAFDSRKRPSFPNLTSFLGCQLADAEEAMYQNVDGRVSECPHTYQNRRPFSREMDLGLLSPQAQVEDS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
100
Horse
91
Dog
91
Chicken
91
Rabbit
91
Pig
82
Bovine
82
Zebrafish
64
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P36888 As Substrate

Site PTM Type Enzyme
N43 N-Glycosylation
N100 N-Glycosylation
N151 N-Glycosylation
Y166 Phosphorylation
N306 N-Glycosylation
N323 N-Glycosylation
T343 Phosphorylation
N351 N-Glycosylation
N354 N-Glycosylation
Y416 Phosphorylation
Y572 Phosphorylation P36888 (FLT3)
S574 Phosphorylation
Y589 Phosphorylation P36888 (FLT3)
Y591 Phosphorylation P36888 (FLT3) , P11309 (PIM1)
Y597 Phosphorylation P36888 (FLT3)
Y599 Phosphorylation P36888 (FLT3)
K602 Ubiquitination
K614 Ubiquitination
K623 Ubiquitination
Y630 Phosphorylation
K634 Ubiquitination
K644 Ubiquitination
K719 Ubiquitination
Y726 Phosphorylation P36888 (FLT3)
T728 Phosphorylation
S759 Phosphorylation
Y768 Phosphorylation P36888 (FLT3)
K772 Ubiquitination
Y793 Phosphorylation P36888 (FLT3)
S840 Phosphorylation
Y842 Phosphorylation P36888 (FLT3)
Y919 Phosphorylation
K932 Ubiquitination
S935 Phosphorylation
Y955 Phosphorylation P36888 (FLT3)
T968 Phosphorylation
Y969 Phosphorylation P36888 (FLT3)
S993 Phosphorylation

PTMs - P36888 As Enzyme

Substrate Site Source
P29353-7 (SHC1) Y239 Uniprot
P29353-7 (SHC1) Y240 Uniprot
P29353-7 (SHC1) Y318 Uniprot
P29353 (SHC1) Y349 Uniprot
P29353 (SHC1) Y350 Uniprot
P29353 (SHC1) Y427 Uniprot
P35222 (CTNNB1) Y654 Uniprot
P36888 (FLT3) Y572 Uniprot
P36888 (FLT3) Y589 Uniprot
P36888 (FLT3) Y591 Uniprot
P36888 (FLT3) Y597 Uniprot
P36888-1 (FLT3) Y599 Uniprot
P36888 (FLT3) Y726 Uniprot
P36888 (FLT3) Y768 Uniprot
P36888 (FLT3) Y793 Uniprot
P36888 (FLT3) Y842 Uniprot
P36888 (FLT3) Y955 Uniprot
P36888 (FLT3) Y969 Uniprot
Q15118 (PDK1) Y243 Uniprot

Research Backgrounds

Function:

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.

PTMs:

N-glycosylated, contains complex N-glycans with sialic acid.

Autophosphorylated on several tyrosine residues in response to FLT3LG binding. FLT3LG binding also increases phosphorylation of mutant kinases that are constitutively activated. Dephosphorylated by PTPRJ/DEP-1, PTPN1, PTPN6/SHP-1, and to a lesser degree by PTPN12. Dephosphorylation is important for export from the endoplasmic reticulum and location at the cell membrane.

Rapidly ubiquitinated by UBE2L6 and the E3 ubiquitin-protein ligase SIAH1 after autophosphorylation, leading to its proteasomal degradation.

Subcellular Location:

Membrane>Single-pass type I membrane protein. Endoplasmic reticulum lumen.
Note: Constitutively activated mutant forms with internal tandem duplications are less efficiently transported to the cell surface and a significant proportion is retained in an immature form in the endoplasmic reticulum lumen. The activated kinase is rapidly targeted for degradation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in bone marrow, in hematopoietic stem cells, in myeloid progenitor cells and in granulocyte/macrophage progenitor cells (at protein level). Detected in bone marrow, liver, thymus, spleen and lymph node, and at low levels in kidney and pancreas. Highly expressed in T-cell leukemia.

Subunit Structure:

Monomer in the absence of bound FLT3LG. Homodimer in the presence of bound FLT3LG. Interacts with FIZ1 following ligand activation (By similarity). Interacts with FES, FER, LYN, FGR, HCK, SRC and GRB2. Interacts with PTPRJ/DEP-1 and PTPN11/SHP2. Interacts with RNF115 and RNF126 (By similarity).

(Microbial infection) Interacts with human cytomegalovirus protein UL7.

Family&Domains:

The juxtamembrane autoregulatory region is important for normal regulation of the kinase activity and for maintaining the kinase in an inactive state in the absence of bound ligand. Upon tyrosine phosphorylation, it mediates interaction with the SH2 domains of numerous signaling partners. In-frame internal tandem duplications (ITDs) result in constitutive activation of the kinase. The activity of the mutant kinase can be stimulated further by FLT3LG binding.

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

References

1). PU.1 promotes development of rheumatoid arthritis via repressing FLT3 in macrophages and fibroblast-like synoviocytes. Annals of the Rheumatic Diseases, 2023 (PubMed: 36198439) [IF=27.4]

Application: WB    Species: Human    Sample:

Figure 1 Expression of PU.1 and FLT3 in patients with RA. (A) IHC staining of PU.1 expression in the joint synovium of healthy donors and patients with OA and RA. (B) Western blotting results of PU.1 expression in joint synovium of patients with OA and RA. (C) Double-staining immunofluorescence shows the co-localisation of PU.1/Vimetin in the synovium of patients with RA. (D) IHC staining verifies the upregulation of PU.1 in the hyperplasia joint synovium of CIA mice. (E) IHC staining of the expression levels of FLT3 and p-FLT3 in the joint synovium of healthy donors and patients with OA and RA. (F) Western blotting results of FLT3 and p-FLT3 expression in joint synovium of patients with OA and RA. CIA, collagen-induced arthritis; FLT3, FMS-like tyrosine kinase 3; IHC, immunohistochemical; OA, osteoarthritis; RA, rheumatoid arthritis.

2). FMS-Related Tyrosine Kinase 3 Ligand Promotes Radioresistance in Esophageal Squamous Cell Carcinoma. Frontiers in Pharmacology, 2021 (PubMed: 34040525) [IF=5.6]

Application: WB    Species: Mice    Sample: ESCC cells

FIGURE 5 FL induces radioresistance in ESCC cells through Flt-3/AKT/Bad signal pathway. (A) mRNA levels of Flt-3 receptor with or without IR in ESCC cells. (B,C) Expression and the quantitative analysis results of Flt-3 and P-Flt-3 in ESCC cells after different doses (0, 2, 4, 6, 8, and 10 Gy) radiation. (D,E) Expression and the quantitative analysis results of Flt-3 and P-Flt-3 in ESCC cells administrated with 200 ng/ml FL for different time after 24 h of 8 Gy radiation. (F,G) The levels and the quantitative analysis results of P-Flt-3/P-PI3K/P-AKT signal proteins and apoptosis-related proteins Bad and P-Bad in PBS groups or 200 ng/ml FL administration for 30 min groups after 24 h of 8 Gy radiation. GAPDH is an internal reference. (H) IHC images of nude mice and IHC analysis of P-Flt-3 and Ki 67 in tumor sections of KYSE150 bearing nude mice. Data show mean ± SEM *p < 0.05.

Application: IHC    Species: mouse    Sample: tumor

FIGURE 5 | FL induces radioresistance in ESCC cells through Flt-3/AKT/Bad signal pathway.(H) IHC images of nude mice and IHC analysis of P-Flt-3 and Ki 67 in tumor sections of KYSE150 bearing nude mice. Data show mean ± SEM *p < 0.05.

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