Phospho-CAMKK1/2 (Ser458/Ser495) Antibody - #AF8136
| Product: | Phospho-CAMKK1/2 (Ser458/Ser495) Antibody |
| Catalog: | AF8136 |
| Description: | Rabbit polyclonal antibody to Phospho-CAMKK1/2 (Ser458/Ser495) |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 64KD; 56kD,65kD(Calculated). |
| Uniprot: | Q8N5S9 | Q96RR4 |
| RRID: | AB_2801648 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF8136, RRID:AB_2801648.
Fold/Unfold
Ca2+/calmodulin-dependent protein kinase kinase alpha; Calcium/calmodulin dependent protein kinase kinase; Calcium/calmodulin dependent protein kinase kinase 1 alpha; Calcium/calmodulin dependent protein kinase kinase 1; Calcium/calmodulin dependent protein kinase kinase alpha; Calcium/calmodulin-dependent protein kinase kinase 1; Calcium/calmodulin-dependent protein kinase kinase alpha; CaM kinase kinase alpha; CaM-kinase IV kinase; CaM-kinase kinase 1; CaM-kinase kinase alpha; CaM-KK 1; CaM-KK alpha; CaMKK 1; CaMKK alpha; CAMKK alpha protein; CaMKK; Camkk1; CAMKKA; DKFZp761M0423; KKCC1_HUMAN; MGC34095; OTTMUSP00000006419; Calcium/calmodulin dependent protein kinase beta; Calcium/calmodulin dependent protein kinase kinase 2; Calcium/calmodulin dependent protein kinase kinase 2 beta; Calcium/calmodulin dependent protein kinase kinase beta; Calcium/calmodulin-dependent protein kinase kinase 2; Calcium/calmodulin-dependent protein kinase kinase beta; CaM kinase kinase beta; CaM KK beta; CaM-kinase kinase 2; CaM-kinase kinase beta; CaM-KK 2; CaM-KK beta; CaMKK 2; CAMKK; CaMKK beta; CAMKK beta protein; Camkk2; CAMKKB; KKCC2_HUMAN;
Immunogens
Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.
- Q8N5S9 KKCC1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEGGPAVCCQDPRAELVERVAAIDVTHLEEADGGPEPTRNGVDPPPRARAASVIPGSTSRLLPARPSLSARKLSLQERPAGSYLEAQAGPYATGPASHISPRAWRRPTIESHHVAISDAEDCVQLNQYKLQSEIGKGAYGVVRLAYNESEDRHYAMKVLSKKKLLKQYGFPRRPPPRGSQAAQGGPAKQLLPLERVYQEIAILKKLDHVNVVKLIEVLDDPAEDNLYLVFDLLRKGPVMEVPCDKPFSEEQARLYLRDVILGLEYLHCQKIVHRDIKPSNLLLGDDGHVKIADFGVSNQFEGNDAQLSSTAGTPAFMAPEAISDSGQSFSGKALDVWATGVTLYCFVYGKCPFIDDFILALHRKIKNEPVVFPEEPEISEELKDLILKMLDKNPETRIGVPDIKLHPWVTKNGEEPLPSEEEHCSVVEVTEEEVKNSVRLIPSWTTVILVKSMLRKRSFGNPFEPQARREERSMSAPGNLLVKEGFGEGGKSPELPGVQEDEAAS
- Q96RR4 KKCC2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSCVSSQPSSNRAAPQDELGGRGSSSSESQKPCEALRGLSSLSIHLGMESFIVVTECEPGCAVDLGLARDRPLEADGQEVPLDTSGSQARPHLSGRKLSLQERSQGGLAAGGSLDMNGRCICPSLPYSPVSSPQSSPRLPRRPTVESHHVSITGMQDCVQLNQYTLKDEIGKGSYGVVKLAYNENDNTYYAMKVLSKKKLIRQAGFPRRPPPRGTRPAPGGCIQPRGPIEQVYQEIAILKKLDHPNVVKLVEVLDDPNEDHLYMVFELVNQGPVMEVPTLKPLSEDQARFYFQDLIKGIEYLHYQKIIHRDIKPSNLLVGEDGHIKIADFGVSNEFKGSDALLSNTVGTPAFMAPESLSETRKIFSGKALDVWAMGVTLYCFVFGQCPFMDERIMCLHSKIKSQALEFPDQPDIAEDLKDLITRMLDKNPESRIVVPEIKLHPWVTRHGAEPLPSEDENCTLVEVTEEEVENSVKHIPSLATVILVKTMIRKRSFGNPFEGSRREERSLSAPGNLLTKKPTRECESLSELKEARQRRQPPGHRPAPRGGGGSALVRGSPCVESCWAPAPGSPARMHPLRPEEAMEPE
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8N5S9/Q96RR4 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| T85 | Phosphorylation | Q96RR4 (CAMKK2) | Uniprot |
| S95 | Phosphorylation | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| S125 | Phosphorylation | Uniprot | |
| Y128 | Phosphorylation | Uniprot | |
| S129 | Phosphorylation | Q00535 (CDK5) , P49841 (GSK3B) , P49840 (GSK3A) | Uniprot |
| S132 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | P49840 (GSK3A) , P49841 (GSK3B) , Q00535 (CDK5) | Uniprot |
| S136 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | P49841 (GSK3B) , P49840 (GSK3A) , Q00535 (CDK5) | Uniprot |
| T145 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| Y183 | Phosphorylation | Uniprot | |
| Y190 | Phosphorylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| Y234 | Phosphorylation | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| T350 | Phosphorylation | Uniprot | |
| K403 | Ubiquitination | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| K429 | Ubiquitination | Uniprot | |
| K441 | Ubiquitination | Uniprot | |
| T483 | Phosphorylation | Q96RR4 (CAMKK2) | Uniprot |
| K488 | Ubiquitination | Uniprot | |
| S495 | Phosphorylation | Uniprot | |
| S509 | Phosphorylation | Uniprot | |
| S511 | Phosphorylation | P53355 (DAPK1) | Uniprot |
| S572 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| S52 | Phosphorylation | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| S69 | Phosphorylation | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| S74 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S82 | Phosphorylation | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| T93 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| R102 | Methylation | Uniprot | |
| T108 | Phosphorylation | Uniprot | |
| K277 | Ubiquitination | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| T446 | Phosphorylation | Uniprot | |
| S452 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| R469 | Methylation | Uniprot | |
| R472 | Methylation | Uniprot | |
| S473 | Phosphorylation | Uniprot | |
| S475 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S492 | Phosphorylation | Uniprot |
PTMs - Q8N5S9/Q96RR4 As Enzyme
Research Backgrounds
Calcium/calmodulin-dependent protein kinase that belongs to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Phosphorylates CAMK1, CAMK1D, CAMK1G and CAMK4. Involved in regulating cell apoptosis. Promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death.
Appears to be autophosphorylated in a Ca(2+)/calmodulin-dependent manner. Phosphorylated at multiple sites by PRCAKA/PKA. Phosphorylation of Ser-458 is blocked upon binding to Ca(2+)/calmodulin. In vitro, phosphorylated by CAMK1 and CAMK4 (By similarity).
Cytoplasm. Nucleus.
Interacts with CAMK4 and calmodulin.
The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.
The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Efficiently phosphorylates 5'-AMP-activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca(2+) signals (By similarity). Seems to be involved in hippocampal activation of CREB1 (By similarity). May play a role in neurite growth. Isoform 3 may promote neurite elongation, while isoform 1 may promoter neurite branching.
Autophosphorylated and phosphorylated by PKA. Each isoform may show a different pattern of phosphorylation.
Nucleus. Cytoplasm. Cell projection>Neuron projection.
Note: Predominantly nuclear in unstimulated cells, relocalizes into cytoplasm and neurites after forskolin induction.
Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.
Interacts with calmodulin.
The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.
The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Research Fields
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Human Diseases > Substance dependence > Alcoholism.
· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)
· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
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