DGKH Antibody - #AF0611
| Product: | DGKH Antibody |
| Catalog: | AF0611 |
| Description: | Rabbit polyclonal antibody to DGKH |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 134kDa; 135kD(Calculated). |
| Uniprot: | Q86XP1 |
| RRID: | AB_2834300 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF0611, RRID:AB_2834300.
Fold/Unfold
DAG kinase eta; DGK eta; Diacylglycerol kinase eta; Diglyceride kinase eta;
Immunogens
Isoform 2 is ubiquitously expressed. Isoform 1 is expressed only in testis, kidney and colon.
- Q86XP1 DGKH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGAGGQHHPPGAAGGAAAGAGAAVTSAAASAGPGEDSSDSEAEQEGPQKLIRKVSTSGQIRTKTSIKEGQLLKQTSSFQRWKKRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAEASTKNANNSFTIITPFRRLMLCAENRKEMEDWISSLKSVQTREPYEVAQFNVEHFSGMHNWYACSHARPTFCNVCRESLSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGVAMPHQWLEGNLPVSAKCAVCDKTCGSVLRLQDWKCLWCKTMVHTACKDLYHPICPLGQCKVSIIPPIALNSTDSDGFCRATFSFCVSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDNFRILVCGGDGSVGWVLSEIDKLNLNKQCQLGVLPLGTGNDLARVLGWGGSYDDDTQLPQILEKLERASTKMLDRWSIMTYELKLPPKASLLPGPPEASEEFYMTIYEDSVATHLTKILNSDEHAVVISSAKTLCETVKDFVAKVEKTYDKTLENAVVADAVASKCSVLNEKLEQLLQALHTDSQAAPVLPGLSPLIVEEDAVESSSEESLGESKEQLGDDVTKPSSQKAVKPREIMLRANSLKKAVRQVIEEAGKVMDDPTVHPCEPANQSSDYDSTETDESKEEAKDDGAKESITVKTAPRSPDARASYGHSQTDSVPGPAVAASKENLPVLNTRIICPGLRAGLAASIAGSSIINKMLLANIDPFGATPFIDPDLDSVDGYSEKCVMNNYFGIGLDAKISLEFNNKREEHPEKCRSRTKNLMWYGVLGTRELLQRSYKNLEQRVQLECDGQYIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDIFAAPSFDDKILEVVAIFDSMQMAVSRVIKLQHHRIAQCRTVKITIFGDEGVPVQVDGEAWVQPPGIIKIVHKNRAQMLTRDRAFESTLKSWEDKQKCDSGKPVLRTHLYIHHAIDLATEEVSQMQLCSQAAEELITRICDAATIHCLLEQELAHAVNACSHALNKANPRCPESLTRDTATEIAINVKALYNETESLLVGRVPLQLESPHEERVSNALHSVEVELQKLTEIPWLYYILHPNEDEEPPMDCTKRNNRSTVFRIVPKFKKEKVQKQKTSSQPVQKWGTEEVAAWLDLLNLGEYKDIFIRHDIRGAELLHLERRDLKDLGIPKVGHVKRILQGIKELGRSTPQSEV
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q86XP1 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S56 | Phosphorylation | Uniprot | |
| T57 | Phosphorylation | Uniprot | |
| T65 | Phosphorylation | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| K266 | Methylation | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| K408 | Ubiquitination | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| T451 | Phosphorylation | Uniprot | |
| K513 | Ubiquitination | Uniprot | |
| K532 | Ubiquitination | Uniprot | |
| T533 | Phosphorylation | Uniprot | |
| S545 | Phosphorylation | Uniprot | |
| K546 | Ubiquitination | Uniprot | |
| S608 | Phosphorylation | Uniprot | |
| S623 | Phosphorylation | Uniprot | |
| Y656 | Phosphorylation | Uniprot | |
| T661 | Phosphorylation | Uniprot | |
| S664 | Phosphorylation | Uniprot | |
| T681 | Phosphorylation | Uniprot | |
| S685 | Phosphorylation | Uniprot | |
| S691 | Phosphorylation | Uniprot | |
| T717 | Phosphorylation | Uniprot | |
| K822 | Ubiquitination | Uniprot | |
| K957 | Ubiquitination | Uniprot | |
| K1033 | Ubiquitination | Uniprot | |
| S1075 | Phosphorylation | Uniprot | |
| K1191 | Methylation | Uniprot |
Research Backgrounds
Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization.
Phosphorylated; does not inhibit catalytic activity.
Cytoplasm. Cell membrane.
Note: Translocated from the cytoplasm to endosomes in response to stress stimuli (PubMed:12810723). Isoform 2 is rapidly relocated back to the cytoplasm upon removal of stress stimuli, whereas isoform 1 exhibits sustained endosomal association (PubMed:12810723). Translocates from the cytoplasm to the cell membrane in the presence of active GTP-bound form of HRAS (PubMed:19710016).
Isoform 2 is ubiquitously expressed. Isoform 1 is expressed only in testis, kidney and colon.
Isoform 1 forms homooligomers through the SAM domain. Isoform 1 is also able to form heterooligomers with SAM domain-containing isoforms of DGKD. Oligomerization of isoform 1 inhibits its catalytic activity. Interacts with RAF1 and BRAF.
Belongs to the eukaryotic diacylglycerol kinase family.
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