Phospho-DNA Ligase IV (Thr650) Antibody - #AF8089

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Product: Phospho-DNA Ligase IV (Thr650) Antibody
Catalog: AF8089
Description: Rabbit polyclonal antibody to Phospho-DNA Ligase IV (Thr650)
Application: WB IHC
Reactivity: Human, Mouse
Mol.Wt.: 103 KD; 104kD(Calculated).
Uniprot: P49917
RRID: AB_2840152

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
Phospho-DNA Ligase IV (Thr650) Antibody detects endogenous levels of DNA Ligase IV only when phosphorylated at Thr650.
RRID:
AB_2840152
Cite Format: Affinity Biosciences Cat# AF8089, RRID:AB_2840152.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DNA ligase IV ATP dependent; DNA joinase; DNA ligase 4; DNA ligase IV; DNA repair enzyme; DNLI4_HUMAN; LIG 4; LIG4; LIG4S; Ligase IV; Ligase IV DNA ATP dependent; Polydeoxyribonucleotide synthase [ATP] 4; Polydeoxyribonucleotide synthase 4; Polydeoxyribonucleotide synthase; Polynucleotide ligase; Sealase;

Immunogens

Immunogen:
Uniprot:

P49917(DNLI4_HUMAN) >>Visit HPA database.

Gene(ID):
LIG4(3981)
Expression:
P49917 DNLI4_HUMAN:

Testis, thymus, prostate and heart.

Sequence:
MAASQTSQTVASHVPFADLCSTLERIQKSKGRAEKIRHFREFLDSWRKFHDALHKNHKDVTDSFYPAMRLILPQLERERMAYGIKETMLAKLYIELLNLPRDGKDALKLLNYRTPTGTHGDAGDFAMIAYFVLKPRCLQKGSLTIQQVNDLLDSIASNNSAKRKDLIKKSLLQLITQSSALEQKWLIRMIIKDLKLGVSQQTIFSVFHNDAAELHNVTTDLEKVCRQLHDPSVGLSDISITLFSAFKPMLAAIADIEHIEKDMKHQSFYIETKLDGERMQMHKDGDVYKYFSRNGYNYTDQFGASPTEGSLTPFIHNAFKADIQICILDGEMMAYNPNTQTFMQKGTKFDIKRMVEDSDLQTCYCVFDVLMVNNKKLGHETLRKRYEILSSIFTPIPGRIEIVQKTQAHTKNEVIDALNEAIDKREEGIMVKQPLSIYKPDKRGEGWLKIKPEYVSGLMDELDILIVGGYWGKGSRGGMMSHFLCAVAEKPPPGEKPSVFHTLSRVGSGCTMKELYDLGLKLAKYWKPFHRKAPPSSILCGTEKPEVYIEPCNSVIVQIKAAEIVPSDMYKTGCTLRFPRIEKIRDDKEWHECMTLDDLEQLRGKASGKLASKHLYIGGDDEPQEKKRKAAPKMKKVIGIIEHLKAPNLTNVNKISNIFEDVEFCVMSGTDSQPKPDLENRIAEFGGYIVQNPGPDTYCVIAGSENIRVKNIILSNKHDVVKPAWLLECFKTKSFVPWQPRFMIHMCPSTKEHFAREYDCYGDSYFIDTDLNQLKEVFSGIKNSNEQTPEEMASLIADLEYRYSWDCSPLSMFRRHTVYLDSYAVINDLSTKNEGTRLAIKALELRFHGAKVVSCLAEGVSHVIIGEDHSRVADFKAFRRTFKRKFKILKESWVTDSIDKCELQEENQYLI

PTMs - P49917 As Substrate

Site PTM Type Enzyme
R40 Methylation
R47 Methylation
K55 Ubiquitination
K85 Ubiquitination
K169 Ubiquitination
S199 Phosphorylation Q13535 (ATR) , Q13315 (ATM)
S267 Phosphorylation
K273 Ubiquitination
K283 Ubiquitination
Y288 Phosphorylation
K289 Ubiquitination
T347 Phosphorylation
S358 Phosphorylation
T362 Phosphorylation
K376 Ubiquitination
K411 Ubiquitination
K424 Ubiquitination
K432 Methylation
K432 Ubiquitination
K439 Ubiquitination
K442 Methylation
Y454 Phosphorylation
K513 Ubiquitination
K521 Ubiquitination
K571 Ubiquitination
K588 Ubiquitination
K613 Ubiquitination
K645 Ubiquitination
T650 Phosphorylation P78527 (PRKDC)
S668 Phosphorylation P78527 (PRKDC)
S672 Phosphorylation P78527 (PRKDC)
K710 Ubiquitination
K722 Ubiquitination
K733 Ubiquitination
K775 Ubiquitination
K841 Ubiquitination
K876 Ubiquitination
K890 Ubiquitination

Research Backgrounds

Function:

Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Testis, thymus, prostate and heart.

Subunit Structure:

Interacts with XRCC4. The LIG4-XRCC4 complex has probably a 1:2 stoichiometry. The LIG4-XRCC4 complex associates in a DNA-dependent manner with the DNA-PK complex composed of PRKDC, XRCC6/Ku70 and XRCC5/Ku86 to form the core non-homologous end joining (NHEJ) complex. Additional components of the NHEJ complex include NHEJ1/XLF and PAXX. Interacts with APLF.

Family&Domains:

Belongs to the ATP-dependent DNA ligase family.

Research Fields

· Genetic Information Processing > Replication and repair > Non-homologous end-joining.

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