Phospho-POU2F1/OCT1 (Ser385) Antibody

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Product:	Phospho-POU2F1/OCT1 (Ser385) Antibody
Catalog:	AF8088
Description:	Rabbit polyclonal antibody to Phospho-POU2F1/OCT1 (Ser385)
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	76KD; 76kD,51kD(Calculated).
Uniprot:	P14859 \| P09086
RRID:	AB_2840151

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

Phospho-POU2F1/OCT1 (Ser385) Antibody detects endogenous levels of POU2F1/OCT1 only when phosphorylated at Ser385.

RRID:

AB_2840151
Cite Format: Affinity Biosciences Cat# AF8088, RRID:AB_2840151.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ42836; NF A1; NF-A1; Oct 1; Oct 1B; Oct-1; OCT1; Octamer binding protein 1; Octamer binding transcription factor 1; Octamer-binding protein 1; Octamer-binding transcription factor 1; OTF 1; OTF-1; OTF1; OTTHUMP00000032348; OTTHUMP00000032350; OTTHUMP00000032351; PO21; PO2F1; PO2F1_HUMAN; POU class 2 homeobox 1; POU domain class 2 transcription factor 1; POU domain, class 2, transcription factor 1; POU2F1; class 2; Lymphoid restricted immunoglobulin octamer binding protein; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; NF A2; Oct 2; Oct-2; Octamer binding transcription factor 2; Octamer-binding protein 2; Octamer-binding transcription factor 2; OTF-2; OTF2; PO2F2_HUMAN; POU domain; POU domain class 2 transcription factor 2; POU2F2; transcription factor 2;

Immunogens

Immunogen:

Uniprot:

P14859(PO2F1_HUMAN) >>Visit HPA database.

P09086(PO2F2_HUMAN) >>Visit HPA database.

Gene(ID):

POU2F1(5451) | POU2F2(5452)

Expression:

P14859 PO2F1_HUMAN:

Ubiquitous. Isoform 2 is lymphocyte-specific.

P09086 PO2F2_HUMAN:

Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.

Sequence:

P14859 PO2F1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNNPSETSKPSMESGDGNTGTQTNGLDFQKQPVPVGGAISTAQAQAFLGHLHQVQLAGTSLQAAAQSLNVQSKSNEESGDSQQPSQPSQQPSVQAAIPQTQLMLAGGQITGLTLTPAQQQLLLQQAQAQAQLLAAAVQQHSASQQHSAAGATISASAATPMTQIPLSQPIQIAQDLQQLQQLQQQNLNLQQFVLVHPTTNLQPAQFIISQTPQGQQGLLQAQNLLTQLPQQSQANLLQSQPSITLTSQPATPTRTIAATPIQTLPQSQSTPKRIDTPSLEEPSDLEELEQFAKTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWLNDAENLSSDSSLSSPSALNSPGIEGLSRRRKKRTSIETNIRVALEKSFLENQKPTSEEITMIADQLNMEKEVIRVWFCNRRQKEKRINPPSSGGTSSSPIKAIFPSPTSLVATTPSLVTSSAATTLTVSPVLPLTSAAVTNLSVTGTSDTTSNNTATVISTAPPASSAVTSPSLSPSPSASASTSEASSASETSTTQTTSTPLSSPLGTSQVMVTASGLQTAAAAALQGAAQLPANASLAAMAAAAGLNPSLMAPSQFAAGGALLSLNPGTLSGALSPALMSNSTLATIQALASGGSLPITSLDATGNLVFANAGGAPNIVTAPLFLNPQNLSLLTSNPVSLVSAAAASAGNSAPVASLHATSTSAESIQNSLFTVASASGAASTTTTASKAQ

P09086 PO2F2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVHSSMGAPEIRMSKPLEAEKQGLDSPSEHTDTERNGPDTNHQNPQNKTSPFSVSPTGPSTKIKAEDPSGDSAPAAPLPPQPAQPHLPQAQLMLTGSQLAGDIQQLLQLQQLVLVPGHHLQPPAQFLLPQAQQSQPGLLPTPNLFQLPQQTQGALLTSQPRAGLPTQAVTRPTLPDPHLSHPQPPKCLEPPSHPEEPSDLEELEQFARTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWLNDAETMSVDSSLPSPNQLSSPSLGFDGLPGRRRKKRTSIETNVRFALEKSFLANQKPTSEEILLIAEQLHMEKEVIRVWFCNRRQKEKRINPCSAAPMLPSPGKPASYSPHMVTPQGGAGTLPLSQASSSLSTTVTTLSSAVGTLHPSRTAGGGGGGGGAAPPLNSIPSVTPPPPATTNSTNPSPQGSHSAIGLSGLNPSTGPGLWWNPAPYQP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P14859/P09086 As Substrate

P14859
P09086

Site	PTM Type	Enzyme	Source
S78	Phosphorylation	P78527 (PRKDC)	Uniprot
S81	Phosphorylation	P78527 (PRKDC)	Uniprot
S85	Phosphorylation	P78527 (PRKDC)	Uniprot
S88	Phosphorylation	P78527 (PRKDC)	Uniprot
S92	Phosphorylation	P78527 (PRKDC)	Uniprot
T100	Phosphorylation	P78527 (PRKDC)	Uniprot
S141	Phosphorylation	P78527 (PRKDC)	Uniprot
S143	Phosphorylation	P78527 (PRKDC)	Uniprot
S147	Phosphorylation	P78527 (PRKDC)	Uniprot
T162	Phosphorylation	P78527 (PRKDC)	Uniprot
S167	Phosphorylation	P78527 (PRKDC)	Uniprot
T226	Phosphorylation	P78527 (PRKDC)	Uniprot
S232	Phosphorylation	P78527 (PRKDC)	Uniprot
T251	Phosphorylation		Uniprot
T255	Phosphorylation		Uniprot
T259	Phosphorylation		Uniprot
S267	Phosphorylation		Uniprot
S269	Phosphorylation	P24941 (CDK2)	Uniprot
T270	Phosphorylation	P24941 (CDK2)	Uniprot
K272	Acetylation		Uniprot
K272	Ubiquitination		Uniprot
T276	Phosphorylation		Uniprot
S278	Phosphorylation		Uniprot
S283	Phosphorylation		Uniprot
K293	Ubiquitination		Uniprot
K301	Ubiquitination		Uniprot
K315	Ubiquitination		Uniprot
S335	Phosphorylation	Q9HC98 (NEK6) , Q9Y478 (PRKAB1) , Q13131 (PRKAA1)	Uniprot
S366	Phosphorylation		Uniprot
S370	Phosphorylation		Uniprot
S385	Phosphorylation	Q13131 (PRKAA1) , P17612 (PRKACA) , P50613 (CDK7)	Uniprot
T445	Phosphorylation		Uniprot
S446	Phosphorylation		Uniprot
S447	Phosphorylation		Uniprot
S448	Phosphorylation		Uniprot

Site	PTM Type	Source
S4	Phosphorylation	Uniprot
S5	Phosphorylation	Uniprot
S14	Phosphorylation	Uniprot
S26	Phosphorylation	Uniprot
S53	Phosphorylation	Uniprot
S55	Phosphorylation	Uniprot
S192	O-Glycosylation	Uniprot
K216	Ubiquitination	Uniprot
K230	Ubiquitination	Uniprot
S250	Phosphorylation	Uniprot
S272	O-Glycosylation	Uniprot
S275	O-Glycosylation	Uniprot
T302	O-Glycosylation	Uniprot
T306	Phosphorylation	Uniprot
R309	Methylation	Uniprot
S324	O-Glycosylation	Uniprot
S372	O-Glycosylation	Uniprot
S390	O-Glycosylation	Uniprot
S395	O-Glycosylation	Uniprot

Research Backgrounds

P14859_PO2F1_HUMAN
P09086_PO2F2_HUMAN

Function:

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.

PTMs:

Phosphorylated by PRKDC.

Subcellular Location:

Nucleus.

Tissue Specificity:

Ubiquitous. Isoform 2 is lymphocyte-specific.

Subunit Structure:

Interacts with NR3C1, AR, PGR and HCFC1. Associates with the herpes simplex virus VP16-induced complex; binding to HCFC1 activates the viral transcriptional activator VP16 for association with POU2F1, to form a multiprotein-DNA complex responsible for activating transcription of the viral immediate early genes.

Family&Domains:

Belongs to the POU transcription factor family. Class-2 subfamily.

Function:

Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.

Subcellular Location:

Cytoplasm. Nucleus.

Tissue Specificity:

Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.

Subunit Structure:

Interacts with NR3C1, AR and PGR.

Family&Domains:

Belongs to the POU transcription factor family. Class-2 subfamily.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-POU2F1/OCT1 (Ser385) Antibody - #AF8088

Related products

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Immunogens

Predictions

PTMs - P14859/P09086 As Substrate

Research Backgrounds

Restrictive clause