Phospho-Centaurin beta-1 (Ser554) Antibody - #AF8083

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Product: Phospho-Centaurin beta-1 (Ser554) Antibody
Catalog: AF8083
Description: Rabbit polyclonal antibody to Phospho-Centaurin beta-1 (Ser554)
Application: WB
Reactivity: Human, Mouse
Mol.Wt.: 81KD; 82kD(Calculated).
Uniprot: Q15027
RRID: AB_2840146

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
Phospho-Centaurin beta-1 (Ser554) Antibody detects endogenous levels of Centaurin beta-1 only when phosphorylated at Ser554.
RRID:
AB_2840146
Cite Format: Affinity Biosciences Cat# AF8083, RRID:AB_2840146.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ACAP 1; ACAP1; ACAP1_HUMAN; Arf GAP with coiled coil ANK repeat and PH domains 1; Arf GAP with coiled coil, ankyrin repeat, and pleckstrin homology domains 1; Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1; ArfGAP with coiled-coil, ankyrin repeat and PH domains 1; CENT B1; Centaurin beta 1; Centaurin-beta-1; CENTB 1; Cnt b1; Cnt-b1; Cntb1; KIAA0050;

Immunogens

Immunogen:
Uniprot:

Q15027(ACAP1_HUMAN) >>Visit HPA database.

Gene(ID):
ACAP1(9744)
Expression:
Q15027 ACAP1_HUMAN:

Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.

Sequence:
MTVKLDFEECLKDSPRFRASIELVEAEVSELETRLEKLLKLGTGLLESGRHYLAASRAFVVGICDLARLGPPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAESLEAALTHNAEVPRRRAQEAEEAGAALRTARAGYRGRALDYALQINVIEDKRKFDIMEFVLRLVEAQATHFQQGHEELSRLSQYRKELGAQLHQLVLNSAREKRDMEQRHVLLKQKELGGEEPEPSLREGPGGLVMEGHLFKRASNAFKTWSRRWFTIQSNQLVYQKKYKDPVTVVVDDLRLCTVKLCPDSERRFCFEVVSTSKSCLLQADSERLLQLWVSAVQSSIASAFSQARLDDSPRGPGQGSGHLAIGSAATLGSGGMARGREPGGVGHVVAQVQSVDGNAQCCDCREPAPEWASINLGVTLCIQCSGIHRSLGVHFSKVRSLTLDSWEPELVKLMCELGNVIINQIYEARVEAMAVKKPGPSCSRQEKEAWIHAKYVEKKFLTKLPEIRGRRGGRGRPRGQPPVPPKPSIRPRPGSLRSKPEPPSEDLGSLHPGALLFRASGHPPSLPTMADALAHGADVNWVNGGQDNATPLIQATAANSLLACEFLLQNGANVNQADSAGRGPLHHATILGHTGLACLFLKRGADLGARDSEGRDPLTIAMETANADIVTLLRLAKMREAEAAQGQAGDETYLDIFRDFSLMASDDPEKLSRRSHDLHTL

PTMs - Q15027 As Substrate

Site PTM Type Enzyme
K4 Acetylation
K4 Ubiquitination
K12 Ubiquitination
S14 Phosphorylation
K37 Ubiquitination
K40 Ubiquitination
T43 Phosphorylation
K82 Ubiquitination
K90 Ubiquitination
K114 Ubiquitination
S133 Phosphorylation
K183 Ubiquitination
K185 Ubiquitination
K218 Ubiquitination
K246 Ubiquitination
K248 Ubiquitination
K274 Acetylation
K274 Ubiquitination
S277 Phosphorylation
K281 Ubiquitination
Y297 Phosphorylation
K302 Ubiquitination
T306 Phosphorylation
K318 Ubiquitination
S333 Phosphorylation
S371 Phosphorylation
S386 Phosphorylation
T389 Phosphorylation
S392 Phosphorylation
K456 Ubiquitination
K495 Ubiquitination
K496 Ubiquitination
K506 Ubiquitination
K513 Ubiquitination
K522 Ubiquitination
K545 Ubiquitination
S547 Phosphorylation
S554 Phosphorylation P31749 (AKT1)
K558 Ubiquitination
T711 Phosphorylation
Y712 Phosphorylation
S720 Phosphorylation
K729 Ubiquitination
S731 Phosphorylation
T739 Phosphorylation

Research Backgrounds

Function:

GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.

PTMs:

Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.

Subcellular Location:

Recycling endosome membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.

Subunit Structure:

Banana-shaped homodimer laterally assembling into tetramers, the tetramers further pack helically onto the membrane. Interacts with GTP-bound ARF6. Interacts with third cytoplasmic loop of SLC2A4/GLUT4. Interacts with CLTC. Interacts with GULP1. Forms a complex with GDP-bound ARF6 and GULP1. Interacts with ITGB1; required for ITGB1 recycling.

Family&Domains:

PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes. Only one PH domain of one ACAP1 dimer inserts into the membrane, while the other PH domain acts primaryly to interact with adjacent ACAP1 dimers.

The BAR domain mediates homodimerization, it can neither bind membrane nor impart curvature, but instead requires the neighboring PH domain to achieve these functions.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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