Phospho-MAX (Ser2) Antibody - #AF8078

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Product: Phospho-MAX (Ser2) Antibody
Catalog: AF8078
Description: Rabbit polyclonal antibody to Phospho-MAX (Ser2)
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 34 kDa; 18kD(Calculated).
Uniprot: P61244
RRID: AB_2840141

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 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-MAX (Ser2) Antibody detects endogenous levels of MAX only when phosphorylated at Ser2.
RRID:
AB_2840141
Cite Format: Affinity Biosciences Cat# AF8078, RRID:AB_2840141.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bHLHd4; bHLHd5; bHLHd6; bHLHd7; bHLHd8; Helix loop helix zipper protein; MAX protein; MGC10775; MGC11225; MGC18164; MGC34679; MGC36767; MYC associated factor X; Myc binding novel HLH/LZ protein; Orf 1; Orf1; Protein max;

Immunogens

Immunogen:
Uniprot:

P61244(MAX_HUMAN) >>Visit HPA database.

Gene(ID):
MAX(4149)
Expression:
P61244 MAX_HUMAN:

High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle.

Sequence:
MSDNDDIEVESDEEQPRFQSAADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P61244 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation P68400 (CSNK2A1)
S11 Phosphorylation P68400 (CSNK2A1)
K24 Ubiquitination
K40 Acetylation
K40 Ubiquitination
S42 Phosphorylation
S45 Phosphorylation
K57 Ubiquitination
K66 Acetylation
T68 Phosphorylation
Y70 Phosphorylation
Y73 Phosphorylation
R75 Methylation
K89 Methylation
K89 Ubiquitination
S107 Phosphorylation
Y115 Phosphorylation
S118 Phosphorylation
S121 Phosphorylation
Y123 Phosphorylation
T124 Phosphorylation
S132 Phosphorylation
S138 Phosphorylation
S140 Phosphorylation
S141 Phosphorylation
S142 Phosphorylation
S144 Phosphorylation
K153 Acetylation
K154 Acetylation

Research Backgrounds

Function:

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Represses MYC transcriptional activity from E-box elements.

PTMs:

Reversible lysine acetylation might regulate the nuclear-cytoplasmic shuttling of specific Max complexes.

Subcellular Location:

Nucleus. Cell projection>Dendrite.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle.

Subunit Structure:

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MYC or MAD. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with SPAG9. The heterodimer MYC:MAX interacts with ABI1; the interaction may enhance MYC:MAX transcriptional activity.

Family&Domains:

Belongs to the MAX family.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

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