STRAD Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of extracts from MDA-MB-231 cells, using STRAD Antibody.

AF0572 at 1/100 staining rat testis tissue by IHC-P.

Product:	STRAD Antibody
Catalog:	AF0572
Description:	Rabbit polyclonal antibody to STRAD
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	46kDa; 48kD(Calculated).
Uniprot:	Q7RTN6
RRID:	AB_2834294

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(100%), Dog(92%)

Clonality:

Polyclonal

Specificity:

STRAD Antibody detects endogenous levels of total STRAD.

RRID:

AB_2834294
Cite Format: Affinity Biosciences Cat# AF0572, RRID:AB_2834294.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Protein kinase LYK5; Serologically defined breast cancer antigen NY BR 96; STE20 related adapter protein; STRAD alpha; STRAD;

Immunogens

Immunogen:

Uniprot:

Q7RTN6(STRAA_HUMAN) >>Visit HPA database.

Gene(ID):

STRADA(92335)

Description:

STRAD a pseudokinase which, in complex with CAB39, binds to and activates LKB1. Relocates LKB1 from the nucleus to the cytoplasm. Plays an essential role in LKB1-mediated G1 cell cycle arrest. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.

Sequence:

Q7RTN6 STRAA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSFLVSKPERIRRWVSEKFIVEGLRDLELFGEQPPGDTRRKTNDASSESIASFSKQEVMSSFLPEGGCYELLTVIGKGFEDLMTVNLARYKPTGEYVTVRRINLEACSNEMVTFLQGELHVSKLFNHPNIVPYRATFIADNELWVVTSFMAYGSAKDLICTHFMDGMNELAIAYILQGVLKALDYIHHMGYVHRSVKASHILISVDGKVYLSGLRSNLSMISHGQRQRVVHDFPKYSVKVLPWLSPEVLQQNLQGYDAKSDIYSVGITACELANGHVPFKDMPATQMLLEKLNGTVPCLLDTSTIPAEELTMSPSRSVANSGLSDSLTTSTPRPSNGDSPSHPYHRTFSPHFHHFVEQCLQRNPDARPSASTLLNHSFFKQIKRRASEALPELLRPVTPITNFEGSQSQDHSGIFGLVTNLEELEVDDWEF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Horse

Bovine

Sheep

Dog

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7RTN6 As Substrate

Q7RTN6

Site	PTM Type	Enzyme	Source
S2	Phosphorylation		Uniprot
T42	Phosphorylation		Uniprot
S46	Phosphorylation		Uniprot
S47	Phosphorylation		Uniprot
S49	Phosphorylation		Uniprot
S52	Phosphorylation		Uniprot
K91	Ubiquitination		Uniprot
K239	Ubiquitination		Uniprot
S313	Phosphorylation		Uniprot
T329	Phosphorylation	Q15831 (STK11)	Uniprot
S387	Phosphorylation		Uniprot
T398	Phosphorylation		Uniprot
T419	Phosphorylation	Q15831 (STK11)	Uniprot

Research Backgrounds

Q7RTN6_STRAA_HUMAN

Function:

Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.

Subcellular Location:

Nucleus. Cytoplasm.

Subunit Structure:

Component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity.

Family&Domains:

The protein kinase domain is predicted to be catalytically inactive.

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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STRAD Antibody - #AF0572