Phospho-MAP4 (Ser696) Antibody - #AF8075

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Product: Phospho-MAP4 (Ser696) Antibody
Catalog: AF8075
Description: Rabbit polyclonal antibody to Phospho-MAP4 (Ser696)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 122kDa; 121kD(Calculated).
Uniprot: P27816
RRID: AB_2840138

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 100ul $350 In stock
 200ul $450 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Phospho-MAP4 (Ser696) Antibody detects endogenous levels of MAP4 only when phosphorylated at Ser696.
RRID:
AB_2840138
Cite Format: Affinity Biosciences Cat# AF8075, RRID:AB_2840138.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp779A1753; MAP-4; MAP4; MAP4_HUMAN; MGC8617; Microtubule associated protein 4; Microtubule-associated protein 4; OTTHUMP00000210723; OTTHUMP00000210725; OTTHUMP00000210727; OTTHUMP00000210730;

Immunogens

Immunogen:
Uniprot:

P27816(MAP4_HUMAN) >>Visit HPA database.

Gene(ID):
MAP4(4134)
Sequence:
MADLSLADALTEPSPDIEGEIKRDFIATLEAEAFDDVVGETVGKTDYIPLLDVDEKTGNSESKKKPCSETSQIEDTPSSKPTLLANGGHGVEGSDTTGSPTEFLEEKMAYQEYPNSQNWPEDTNFCFQPEQVVDPIQTDPFKMYHDDDLADLVFPSSATADTSIFAGQNDPLKDSYGMSPCNTAVVPQGWSVEALNSPHSESFVSPEAVAEPPQPTAVPLELAKEIEMASEERPPAQALEIMMGLKTTDMAPSKETEMALAKDMALATKTEVALAKDMESPTKLDVTLAKDMQPSMESDMALVKDMELPTEKEVALVKDVRWPTETDVSSAKNVVLPTETEVAPAKDVTLLKETERASPIKMDLAPSKDMGPPKENKKETERASPIKMDLAPSKDMGPPKENKIVPAKDLVLLSEIEVAQANDIISSTEISSAEKVALSSETEVALARDMTLPPETNVILTKDKALPLEAEVAPVKDMAQLPETEIAPAKDVAPSTVKEVGLLKDMSPLSETEMALGKDVTPPPETEVVLIKNVCLPPEMEVALTEDQVPALKTEAPLAKDGVLTLANNVTPAKDVPPLSETEATPVPIKDMEIAQTQKGISEDSHLESLQDVGQSAAPTFMISPETVTGTGKKCSLPAEEDSVLEKLGERKPCNSQPSELSSETSGIARPEEGRPVVSGTGNDITTPPNKELPPSPEKKTKPLATTQPAKTSTSKAKTQPTSLPKQPAPTTIGGLNKKPMSLASGLVPAAPPKRPAVASARPSILPSKDVKPKPIADAKAPEKRASPSKPASAPASRSGSKSTQTVAKTTTAAAVASTGPSSRSPSTLLPKKPTAIKTEGKPAEVKKMTAKSVPADLSRPKSTSTSSMKKTTTLSGTAPAAGVVPSRVKATPMPSRPSTTPFIDKKPTSAKPSSTTPRLSRLATNTSAPDLKNVRSKVGSTENIKHQPGGGRAKVEKKTEAAATTRKPESNAVTKTAGPIASAQKQPAGKVQIVSKKVSYSHIQSKCGSKDNIKHVPGGGNVQIQNKKVDISKVSSKCGSKANIKHKPGGGDVKIESQKLNFKEKAQAKVGSLDNVGHLPAGGAVKTEGGGSEAPLCPGPPAGEEPAISEAAPEAGAPTSASGLNGHPTLSGGGDQREAQTLDSQIQETSI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Horse
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P27816 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S5 Phosphorylation
T11 Phosphorylation
S14 Phosphorylation
T28 Phosphorylation
T41 Phosphorylation
T45 Phosphorylation
Y47 Phosphorylation
K56 Ubiquitination
T57 Phosphorylation
S60 Phosphorylation
S68 Phosphorylation
S71 Phosphorylation
T76 Phosphorylation
S94 Phosphorylation
T96 Phosphorylation
T97 Phosphorylation
S99 Phosphorylation
T101 Phosphorylation
Y110 Phosphorylation
Y113 Phosphorylation
S116 Phosphorylation
T183 Phosphorylation
S197 Phosphorylation
S200 Phosphorylation
S202 Phosphorylation
S230 Phosphorylation
K254 Ubiquitination
T256 Phosphorylation
K262 Acetylation
K262 Ubiquitination
K269 Acetylation
K269 Sumoylation
K269 Ubiquitination
K276 Acetylation
S280 Phosphorylation
T282 Phosphorylation
T287 Phosphorylation
S295 Phosphorylation
K312 Ubiquitination
K318 Ubiquitination
T324 Phosphorylation
T326 Phosphorylation
S329 Phosphorylation
S330 Phosphorylation
K332 Ubiquitination
T340 Phosphorylation
K346 Acetylation
K346 Ubiquitination
K352 Ubiquitination
T354 Phosphorylation
S358 Phosphorylation
K361 Ubiquitination
S367 Phosphorylation
K368 Acetylation
K368 Ubiquitination
T380 Phosphorylation
S384 Phosphorylation
K387 Ubiquitination
S393 Phosphorylation
K394 Acetylation
K394 Ubiquitination
K400 Ubiquitination
S439 Phosphorylation
S440 Phosphorylation
T442 Phosphorylation
T451 Phosphorylation
K464 Ubiquitination
K476 Acetylation
K476 Ubiquitination
T484 Phosphorylation
K490 Ubiquitination
S495 Phosphorylation
T496 Phosphorylation
K498 Ubiquitination
S507 Phosphorylation
S510 Phosphorylation
T512 Phosphorylation
T521 Phosphorylation
T526 Phosphorylation
K560 Ubiquitination
T565 Phosphorylation
T571 Phosphorylation
K574 Ubiquitination
S580 Phosphorylation
T582 Phosphorylation
T585 Phosphorylation
S602 Phosphorylation
S605 Phosphorylation
S609 Phosphorylation
T620 Phosphorylation
S624 Phosphorylation
T627 Phosphorylation
K633 Acetylation
K634 Ubiquitination
C635 S-Nitrosylation
S636 Phosphorylation
S643 Phosphorylation
K647 Ubiquitination
T686 Phosphorylation
T687 Phosphorylation
S696 Phosphorylation P06493 (CDK1)
K702 Acetylation
K702 Ubiquitination
T706 Phosphorylation
T712 Phosphorylation
S713 Phosphorylation
K718 Acetylation
T719 Phosphorylation
T722 Phosphorylation
S723 Phosphorylation
K726 Ubiquitination
T731 Phosphorylation
S742 Phosphorylation
S745 Phosphorylation
S760 Phosphorylation
S764 Phosphorylation
S768 Phosphorylation
K784 Acetylation
S787 Phosphorylation P06493 (CDK1)
S789 Phosphorylation
S793 Phosphorylation
S797 O-Glycosylation
S797 Phosphorylation
S803 Phosphorylation
T804 Phosphorylation
T806 Phosphorylation
S818 Phosphorylation
T819 Phosphorylation
S822 Phosphorylation
S823 Phosphorylation
S825 Phosphorylation
S827 Phosphorylation
T828 Phosphorylation
K838 Acetylation
K838 Sumoylation
K847 Acetylation
K852 Acetylation
S853 Phosphorylation
S859 Phosphorylation
S863 Phosphorylation
S865 Phosphorylation
S868 Phosphorylation
K871 Acetylation
T872 Phosphorylation
T874 Phosphorylation
S876 Phosphorylation
T878 Phosphorylation
T892 Phosphorylation P06493 (CDK1)
S896 Phosphorylation
S899 Phosphorylation
T900 Phosphorylation
T901 Phosphorylation P06493 (CDK1)
T909 Phosphorylation
K912 Acetylation
S914 Phosphorylation
S915 Phosphorylation
T917 Phosphorylation P06493 (CDK1)
T925 Phosphorylation O14757 (CHEK1)
T927 Phosphorylation
S928 Phosphorylation
K933 Acetylation
K933 Ubiquitination
S937 Phosphorylation
K938 Acetylation
S941 Phosphorylation Q9P0L2 (MARK1)
T942 Phosphorylation
K946 Ubiquitination
K955 Methylation
T960 Phosphorylation
T965 Phosphorylation
T977 Phosphorylation
S983 Phosphorylation
K986 Ubiquitination
K991 Acetylation
K991 Ubiquitination
S996 Phosphorylation
K997 Acetylation
K998 Acetylation
K998 Ubiquitination
S1000 Phosphorylation
Y1001 Phosphorylation
S1002 Phosphorylation
S1006 Phosphorylation
K1007 Ubiquitination
S1010 Phosphorylation
K1011 Methylation
K1015 Ubiquitination
K1028 Acetylation
K1028 Ubiquitination
K1034 Acetylation
S1036 Phosphorylation
S1037 Phosphorylation
K1038 Acetylation
S1041 Phosphorylation
K1042 Acetylation
S1058 Phosphorylation
K1060 Acetylation
K1060 Ubiquitination
K1064 Methylation
K1070 Acetylation
K1070 Ubiquitination
S1073 Phosphorylation Q9P0L2 (MARK1)
T1142 Phosphorylation
S1145 Phosphorylation
T1150 Phosphorylation
S1151 Phosphorylation

Research Backgrounds

Function:

Non-neuronal microtubule-associated protein. Promotes microtubule assembly.

PTMs:

Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Phosphorylation on Ser-787 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 3 is phosphorylated on Ser-337 and Ser-338.

Subcellular Location:

Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with SEPTIN2; this interaction impedes tubulin-binding. Interacts with TRAF3IP1 (By similarity).

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For Research Use Only.
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