Phospho-Hic-5 (Tyr60) Antibody - #AF8064

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Product: Phospho-Hic-5 (Tyr60) Antibody
Catalog: AF8064
Description: Rabbit polyclonal antibody to Phospho-Hic-5 (Tyr60)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 50kDa; 50kD(Calculated).
Uniprot: O43294
RRID: AB_2840127

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 100ul $350 In stock
 200ul $450 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-Hic-5 (Tyr60) Antibody detects endogenous levels of Hic-5 only when phosphorylated at Tyr60.
RRID:
AB_2840127
Cite Format: Affinity Biosciences Cat# AF8064, RRID:AB_2840127.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Androgen receptor coactivator 55 kDa protein; Androgen receptor coactivator ARA55; Androgen receptor-associated protein of 55 kDa; ARA55; Hic-5; Hydrogen peroxide-inducible clone 5 protein; Tgfb1i1; TGFI1_HUMAN; Transforming growth factor beta 1 induced transcript 1; Transforming growth factor beta-1-induced transcript 1 protein; TSC 5;

Immunogens

Immunogen:
Uniprot:

O43294(TGFI1_HUMAN) >>Visit HPA database.

Gene(ID):
TGFB1I1(7041)
Expression:
O43294 TGFI1_HUMAN:

Expressed in platelets, smooth muscle and prostate stromal cells (at protein level).

Sequence:
MEDLDALLSDLETTTSHMPRSGAPKERPAEPLTPPPSYGHQPQTGSGESSGASGDKDHLYSTVCKPRSPKPAAPAAPPFSSSSGVLGTGLCELDRLLQELNATQFNITDEIMSQFPSSKVASGEQKEDQSEDKKRPSLPSSPSPGLPKASATSATLELDRLMASLSDFRVQNHLPASGPTQPPVVSSTNEGSPSPPEPTGKGSLDTMLGLLQSDLSRRGVPTQAKGLCGSCNKPIAGQVVTALGRAWHPEHFVCGGCSTALGGSSFFEKDGAPFCPECYFERFSPRCGFCNQPIRHKMVTALGTHWHPEHFCCVSCGEPFGDEGFHEREGRPYCRRDFLQLFAPRCQGCQGPILDNYISALSALWHPDCFVCRECFAPFSGGSFFEHEGRPLCENHFHARRGSLCATCGLPVTGRCVSALGRRFHPDHFTCTFCLRPLTKGSFQERAGKPYCQPCFLKLFG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43294 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S9 Phosphorylation
T33 Phosphorylation
S37 Phosphorylation
Y38 Phosphorylation
Y60 Phosphorylation Q14289 (PTK2B) , P06241 (FYN)
S61 Phosphorylation
T62 Phosphorylation
S68 Phosphorylation
S122 Phosphorylation
S130 Phosphorylation
S137 Phosphorylation
S140 Phosphorylation
S141 Phosphorylation P28482 (MAPK1)
S143 Phosphorylation
S150 Phosphorylation
S153 Phosphorylation
S164 Phosphorylation
S166 Phosphorylation
S187 Phosphorylation
T188 Phosphorylation
S192 Phosphorylation
S194 Phosphorylation
T199 Phosphorylation
S203 Phosphorylation
S213 Phosphorylation
S216 Phosphorylation
S403 Phosphorylation
T407 Phosphorylation
S418 Phosphorylation
K449 Ubiquitination
K458 Ubiquitination

Research Backgrounds

Function:

Functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. Links various intracellular signaling modules to plasma membrane receptors and regulates the Wnt and TGFB signaling pathways. May also regulate SLC6A3 and SLC6A4 targeting to the plasma membrane hence regulating their activity. In the nucleus, functions as a nuclear receptor coactivator regulating glucocorticoid, androgen, mineralocorticoid and progesterone receptor transcriptional activity. May play a role in the processes of cell growth, proliferation, migration, differentiation and senescence. May have a zinc-dependent DNA-binding activity.

PTMs:

Phosphorylated by gonadotropin-releasing hormone-activated SRC.

Subcellular Location:

Cell junction>Focal adhesion. Nucleus matrix. Cytoplasm>Cytoskeleton.
Note: Associated with the actin cytoskeleton; colocalizes with stress fibers.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in platelets, smooth muscle and prostate stromal cells (at protein level).

Subunit Structure:

Homooligomer. Interacts with CRIP2, HSPB1, ILK, LIMS1, LIMS2, NCK2, NUDT16L1, PAK, PPARG, PTPN12, TCF3, TCF7L2 and VCL. Forms a complex with GIT1 and ARHGEF7. Interacts with AR/androgen receptor in a ligand-dependent manner. Interacts with CSK, LYN, MAPK15, NR3C1, PPARG, PTK2/FAK1, PTK2B/PYK2, SLC6A3, SLC6A4, SMAD3, SRC and talin. Interacts (via LIM zinc-binding domain 2) with CBLC (via RING-type zinc finger); the interaction is direct and enhances CBLC E3 ubiquitin-protein ligase activity.

Family&Domains:

The LIM zinc-binding domains mediate glucocorticoid receptor coactivation and interaction with AR, CRIP2, ILK, LIMS1, NR3C1, PPARG, TCF3, TCF7L2, SLC6A3 and SMAD3. The LIM zinc-binding 2 and LIM zinc-binding 3 domains mediate targeting to focal adhesions and actin stress fibers. The LIM zinc-binding 3 and LIM zinc-binding 4 domains mediate interaction with TRAF4 and MAPK15. The LIM zinc-binding 4 domain mediates interaction with HSPB1, homooligomerization and targeting to the nuclear matrix. The LIM zinc-binding 3 domain mediates interaction with PTPN12.

The LD (leucine and aspartate-rich) motif 3 mediates interaction with GIT1 and functions as a nuclear export signal.

Belongs to the paxillin family.

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