Phospho-Gz alpha (Ser16) Antibody | Affinity Biosciences

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Product:	Phospho-Gz alpha (Ser16) Antibody
Catalog:	AF8062
Description:	Rabbit polyclonal antibody to Phospho-Gz alpha (Ser16)
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	41kDa; 41kD(Calculated).
Uniprot:	P19086
RRID:	AB_2840125

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

Phospho-Gz alpha (Ser16) Antibody detects endogenous levels of Gz alpha only when phosphorylated at Ser16.

RRID:

AB_2840125
Cite Format: Affinity Biosciences Cat# AF8062, RRID:AB_2840125.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

G(x) alpha chain; GNAZ; GNAZ_HUMAN; Guanine nucleotide binding protein (G protein) alpha z polypeptide; Guanine nucleotide binding protein G(z) alpha subunit G(x) alpha chain; Guanine nucleotide binding protein G(z) subunit alpha; Guanine nucleotide-binding protein G(z) subunit alpha; Gz alpha; Gz-alpha; Human Gx alpha gene; Transducin alpha;

Immunogens

Immunogen:

Uniprot:

P19086(GNAZ_HUMAN) >>Visit HPA database.

Gene(ID):

GNAZ(2781)

Sequence:

P19086 GNAZ_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGCRQSSEEKEAARRSRRIDRHLRSESQRQRREIKLLLLGTSNSGKSTIVKQMKIIHSGGFNLEACKEYKPLIIYNAIDSLTRIIRALAALRIDFHNPDRAYDAVQLFALTGPAESKGEITPELLGVMRRLWADPGAQACFSRSSEYHLEDNAAYYLNDLERIAAADYIPTVEDILRSRDMTTGIVENKFTFKELTFKMVDVGGQRSERKKWIHCFEGVTAIIFCVELSGYDLKLYEDNQTSRMAESLRLFDSICNNNWFINTSLILFLNKKDLLAEKIRRIPLTICFPEYKGQNTYEEAAVYIQRQFEDLNRNKETKEIYSHFTCATDTSNIQFVFDAVTDVIIQNNLKYIGLC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Sheep

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P19086 As Substrate

P19086

Site	PTM Type	Enzyme	Source
S16	Phosphorylation	Q05655 (PRKCD) , Q13153 (PAK1) , P17252 (PRKCA)	Uniprot
S27	Phosphorylation	P17252 (PRKCA) , Q05655 (PRKCD)	Uniprot
T41	Phosphorylation		Uniprot
K51	Ubiquitination		Uniprot
S142	Phosphorylation		Uniprot
K189	Ubiquitination		Uniprot
S229	Phosphorylation		Uniprot
Y231	Phosphorylation		Uniprot
T263	Phosphorylation		Uniprot
S264	Phosphorylation		Uniprot
T341	Phosphorylation		Uniprot

Research Backgrounds

P19086_GNAZ_HUMAN

Function:

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.

Subcellular Location:

Membrane>Lipid-anchor.

Subunit Structure:

G-proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with ADGRB2.

Family&Domains:

Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Research Fields

· Organismal Systems > Nervous system > Long-term depression.

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For Research Use Only.
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Phospho-Gz alpha (Ser16) Antibody - #AF8062