Product: Phospho-Syndecan 4 (Ser179) Antibody
Catalog: AF8061
Description: Rabbit polyclonal antibody to Phospho-Syndecan 4 (Ser179)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 22kDa; 22kD(Calculated).
Uniprot: P31431
RRID: AB_2840124

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 100ul $350 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(89%), Dog(89%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Phospho-Syndecan 4 (Ser179) Antibody detects endogenous levels of Syndecan 4 only when phosphorylated at Ser179.
RRID:
AB_2840124
Cite Format: Affinity Biosciences Cat# AF8061, RRID:AB_2840124.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Amphiglycan; MGC22217; OTTHUMP00000031788; ryudocan amphiglycan; Ryudocan; Ryudocan core protein; SDC 4; Sdc4; SDC4_HUMAN; SYND 4; SYND4; syndecan 4; syndecan 4 (amphiglycan, ryudocan); syndecan proteoglycan 4; Syndecan-4; Syndecan4;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P31431 SDC4_HUMAN:

Expressed in epithelial and fibroblastic cells.

Sequence:
MAPARLFALLLFFVGGVAESIRETEVIDPQDLLEGRYFSGALPDDEDVVGPGQESDDFELSGSGDLDDLEDSMIGPEVVHPLVPLDNHIPERAGSGSQVPTEPKKLEENEVIPKRISPVEESEDVSNKVSMSSTVQGSNIFERTEVLAALIVGGIVGILFAVFLILLLMYRMKKKDEGSYDLGKKPIYKKAPTNEFYA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Zebrafish
100
Chicken
100
Dog
89
Rabbit
89
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31431 As Substrate

Site PTM Type Enzyme
S97 O-Glycosylation
T101 O-Glycosylation
K104 Ubiquitination
S117 O-Glycosylation
S126 O-Glycosylation
T134 Phosphorylation
K174 Ubiquitination
S179 Phosphorylation Q05655 (PRKCD)
Y180 Phosphorylation P12931 (SRC)
K190 Ubiquitination
T193 Phosphorylation
Y197 Phosphorylation

Research Backgrounds

Function:

Cell surface proteoglycan that bears heparan sulfate. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.

PTMs:

Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.

Subcellular Location:

Membrane>Single-pass type I membrane protein. Secreted.
Note: Shedding of the ectodomain produces a soluble form.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in epithelial and fibroblastic cells.

Subunit Structure:

Homodimer. Interacts (via its cytoplasmic domain) with GIPC (via its PDZ domain). Interacts (via its cytoplasmic domain) with NUDT16L1 (By similarity). Interacts with CDCP1 and SDCBP.

Family&Domains:

Belongs to the syndecan proteoglycan family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

References

1). Phosphorylation-regulated phase separation of syndecan-4 and syntenin promotes the biogenesis of exosomes. Cell proliferation, 2024 (PubMed: 38601993) [IF=8.5]

Application: WB    Species: Human    Sample: HeLa cells

FIGURE 3 Dephosphorylation of SDC4 increased SDC4 phase sepration and the recruitment of syntenin on the plasma membrane (PM). (A) Confocal laser scanning microscopy (CLSM) images of Staurosporine and PMA regulate SDC4 clusters on the PM of HeLa cells. An anti-SDC4-ecto antibody and a Cy3-labelled secondary antibody were used to detect the location of SDC4. (B) SDC4-eGFP-syntenin cells were stimulated with PKC-activating phorbol ester (PMA) and staurosporine. Dimethyl sulfoxide (DMSO) was used as the control. (C) For the quantification of the subcellular localization of syntenin stimulated with PMA or staurosporine, the number of cells that have syntenin in the cytoplasm and at the plasma membrane was analysed. The y-axis represents the ratio of the number of cells that have syntenin on the membrane to those in the cytoplasm. The results were calculated in 3 independent experiments (**p 

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