Phospho-Syndecan 4 (Ser179) Antibody

IHC
pElisa
Cites

1/4

AF8061 at 1/200 staining Human heart tissue sections by IHC-P.

AF8061 at 1/200 staining Rat ganstric tissue sections by IHC-P.

peptide-ELISA analysis of AF8061.

FIGURE 3 Dephosphorylation of SDC4 increased SDC4 phase sepration and the recruitment of syntenin on the plasma membrane (PM).

Product:	Phospho-Syndecan 4 (Ser179) Antibody
Catalog:	AF8061
Description:	Rabbit polyclonal antibody to Phospho-Syndecan 4 (Ser179)
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	22kDa; 22kD(Calculated).
Uniprot:	P31431
RRID:	AB_2840124

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(89%), Dog(89%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

Phospho-Syndecan 4 (Ser179) Antibody detects endogenous levels of Syndecan 4 only when phosphorylated at Ser179.

RRID:

AB_2840124
Cite Format: Affinity Biosciences Cat# AF8061, RRID:AB_2840124.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Amphiglycan; MGC22217; OTTHUMP00000031788; ryudocan amphiglycan; Ryudocan; Ryudocan core protein; SDC 4; Sdc4; SDC4_HUMAN; SYND 4; SYND4; syndecan 4; syndecan 4 (amphiglycan, ryudocan); syndecan proteoglycan 4; Syndecan-4; Syndecan4;

Immunogens

Immunogen:

Uniprot:

P31431(SDC4_HUMAN) >>Visit HPA database.

Gene(ID):

SDC4(6385)

Expression:

P31431 SDC4_HUMAN:

Expressed in epithelial and fibroblastic cells.

Sequence:

P31431 SDC4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAPARLFALLLFFVGGVAESIRETEVIDPQDLLEGRYFSGALPDDEDVVGPGQESDDFELSGSGDLDDLEDSMIGPEVVHPLVPLDNHIPERAGSGSQVPTEPKKLEENEVIPKRISPVEESEDVSNKVSMSSTVQGSNIFERTEVLAALIVGGIVGILFAVFLILLLMYRMKKKDEGSYDLGKKPIYKKAPTNEFYA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Zebrafish

100

Chicken

100

Dog

Rabbit

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31431 As Substrate

P31431

Site	PTM Type	Enzyme	Source
S97	O-Glycosylation		Uniprot
T101	O-Glycosylation		Uniprot
K104	Ubiquitination		Uniprot
S117	O-Glycosylation		Uniprot
S126	O-Glycosylation		Uniprot
T134	Phosphorylation		Uniprot
K174	Ubiquitination		Uniprot
S179	Phosphorylation	Q05655 (PRKCD)	Uniprot
Y180	Phosphorylation	P12931 (SRC)	Uniprot
K190	Ubiquitination		Uniprot
T193	Phosphorylation		Uniprot
Y197	Phosphorylation		Uniprot

Research Backgrounds

P31431_SDC4_HUMAN

Function:

Cell surface proteoglycan that bears heparan sulfate. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.

PTMs:

Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.

Subcellular Location:

Membrane>Single-pass type I membrane protein. Secreted.
Note: Shedding of the ectodomain produces a soluble form.

Secreted.

Tissue Specificity:

Expressed in epithelial and fibroblastic cells.

Subunit Structure:

Homodimer. Interacts (via its cytoplasmic domain) with GIPC (via its PDZ domain). Interacts (via its cytoplasmic domain) with NUDT16L1 (By similarity). Interacts with CDCP1 and SDCBP.

Family&Domains:

Belongs to the syndecan proteoglycan family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

References

1). Phosphorylation-regulated phase separation of syndecan-4 and syntenin promotes the biogenesis of exosomes. Cell proliferation, 2024 (PubMed: 38601993) [IF=8.5]

Application: WB Species: Human Sample: HeLa cells

FIGURE 3 Dephosphorylation of SDC4 increased SDC4 phase sepration and the recruitment of syntenin on the plasma membrane (PM). (A) Confocal laser scanning microscopy (CLSM) images of Staurosporine and PMA regulate SDC4 clusters on the PM of HeLa cells. An anti-SDC4-ecto antibody and a Cy3-labelled secondary antibody were used to detect the location of SDC4. (B) SDC4-eGFP-syntenin cells were stimulated with PKC-activating phorbol ester (PMA) and staurosporine. Dimethyl sulfoxide (DMSO) was used as the control. (C) For the quantification of the subcellular localization of syntenin stimulated with PMA or staurosporine, the number of cells that have syntenin in the cytoplasm and at the plasma membrane was analysed. The y-axis represents the ratio of the number of cells that have syntenin on the membrane to those in the cytoplasm. The results were calculated in 3 independent experiments (**p

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Phospho-Syndecan 4 (Ser179) Antibody - #AF8061