DOK3 Antibody - #AF0600

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Product: DOK3 Antibody
Catalog: AF0600
Description: Rabbit polyclonal antibody to DOK3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Rabbit, Dog
Mol.Wt.: 58kDa; 53kD(Calculated).
Uniprot: Q7L591
RRID: AB_2834292

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
DOK3 Antibody detects endogenous levels of total DOK3.
RRID:
AB_2834292
Cite Format: Affinity Biosciences Cat# AF0600, RRID:AB_2834292.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Docking protein 3; DOK 3; DOK Like Protein; Dok3; DOK3_HUMAN; DOKL Pending; Downstream of tyrosine kinase 3; p62 DOK Like Protein;

Immunogens

Immunogen:
Uniprot:

Q7L591(DOK3_HUMAN) >>Visit HPA database.

Gene(ID):
DOK3(79930)
Expression:
Q7L591 DOK3_HUMAN:

Expressed in spleen.

Description:
Docking proteins interact with receptor tyrosine kinases and mediate particular biological responses. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP. May modulate Abl function. On tyrosine phosphorylation, interacts with CSK and INPP5D/SHIP via their SH2 domains. Both Tyr-381 and Tyr-398 are required for interaction with INPP5D. Only Tyr-381 is required for interaction with CSK. Binds ABL through the PTB domain and in a kinase-dependent manner. Does not interact with RasGAP. 4 isoforms of the human protein are produced by alternative splicing.
Sequence:
MTRGARLRSDARAQLNQLSLDGGTGSGQKGKCEEFPSSLSSVSPGLEAAALLLAVTMDPLETPIKDGILYQQHVKFGKKCWRKVWALLYAGGPSGVARLESWEVRDGGLGAAGDRSAGPGRRGERRVIRLADCVSVLPADGESCPRDTGAFLLTTTERSHLLAAQHRQAWMGPICQLAFPGTGEASSGSTDAQSPKRGLVPMEENSIYSSWQEVGEFPVVVQRTEAATRCQLKGPALLVLGPDAIQLREAKGTQALYSWPYHFLRKFGSDKGVFSFEAGRRCHSGEGLFAFSTPCAPDLCRAVAGAIARQRERLPELTRPQPCPLPRATSLPSLDTPGELREMPPGPEPPTSRKMHLAEPGPQSLPLLLGPEPNDLASGLYASVCKRASGPPGNEHLYENLCVLEASPTLHGGEPEPHEGPGSRSPTTSPIYHNGQDLSWPGPANDSTLEAQYRRLLELDQVEGTGRPDPQAGFKAKLVTLLSRERRKGPAPCDRP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Rabbit
100
Chicken
70
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7L591 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation
Y89 Phosphorylation
R122 Methylation
S194 Phosphorylation
Y208 Phosphorylation
T329 Phosphorylation
S330 Phosphorylation
S333 Phosphorylation
T336 Phosphorylation
T351 Phosphorylation
S352 Phosphorylation
S364 Phosphorylation
S378 Phosphorylation
Y381 Phosphorylation
S389 Phosphorylation
Y398 Phosphorylation
S407 Phosphorylation
Y432 Phosphorylation
Y453 Phosphorylation
K477 Acetylation
T480 Phosphorylation
S483 Phosphorylation

Research Backgrounds

Function:

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL1 function (By similarity).

PTMs:

Constitutively tyrosine-phosphorylated.

On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL1 kinase (By similarity).

Subcellular Location:

Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in spleen.

Subunit Structure:

On tyrosine phosphorylation, interacts with CSK and INPP5D/SHIP1 via their SH2 domains. Both Tyr-381 and Tyr-398 are required for interaction with INPP5D. Only Tyr-381 is required for interaction with CSK. Binds ABL1 through the PTB domain and in a kinase-dependent manner. Does not interact with RasGAP (By similarity).

Family&Domains:

PTB domain mediates receptor interaction.

Belongs to the DOK family. Type A subfamily.

References

1). Dok3 is involved in cisplatin-induced acute kidney injury via regulation of inflammation and apoptosis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2021 (PubMed: 34245977) [IF=3.1]

Application: IHC    Species: Mouse    Sample: Kidney tissues

Fig.1. Deletion of Dok3 attenuates cisplatin-induced AKI. (A) Level of BUN in the serum. (A) Level of creatinine in the serum; (C) Images of immunohistochemical staining of NGAL; 400 ? magnification. (D) Quantification of immunohistochemical staining of NGAL. (E) Analysis of survival rate of mice. All values are expressed as the mean ± standard error. n ¼ 6, **P < 0.01, and ***P < 0.001.

Application: WB    Species: Mouse    Sample: Kidney tissues

Fig. 4. Overexpression of DOK3 exacerbates apoptosis and inflammation in vitro. (A) Dok3 mRNA expression in mouse kidney tissues. (B) Dok3 protein levels in mouse kidney tissues. (C) Images of immunohistochemical (IHC) staining of DOK3 (400 ? magnification). (D) Images of immunofluorescence staining of DOK3/AQP1 (400 ? magnification). (E) Dok3 protein levels in HK2 cells. (F) Western blot analysis verifying pcDNA-Dok3 expression. (G) PI-Hochest staining of pcDNA-Dok3-transfected HK2 cells with/without cisplatin treatment. (H) LDH level in the cell culture supernatant. (I) TNF- a level in cell culture supernatant. (J) IL-6 level in cell culture supernatant. All values are expressed as the mean ± standard error, *P < 0.05, **P < 0.01, and ***P < 0.001.

Application: IF/ICC    Species: Mouse    Sample: Kidney tissues

Fig. 4. Overexpression of DOK3 exacerbates apoptosis and inflammation in vitro. (A) Dok3 mRNA expression in mouse kidney tissues. (B) Dok3 protein levels in mouse kidney tissues. (C) Images of immunohistochemical (IHC) staining of DOK3 (400 ? magnification). (D) Images of immunofluorescence staining of DOK3/AQP1 (400 ? magnification). (E) Dok3 protein levels in HK2 cells. (F) Western blot analysis verifying pcDNA-Dok3 expression. (G) PI-Hochest staining of pcDNA-Dok3-transfected HK2 cells with/without cisplatin treatment. (H) LDH level in the cell culture supernatant. (I) TNF- a level in cell culture supernatant. (J) IL-6 level in cell culture supernatant. All values are expressed as the mean ± standard error, *P < 0.05, **P < 0.01, and ***P < 0.001.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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