Product: Phospho-CFTR (Ser737) Antibody
Catalog: AF8042
Description: Rabbit polyclonal antibody to Phospho-CFTR (Ser737)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 168kDa; 168kD(Calculated).
Uniprot: P13569
RRID: AB_2840105

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(89%), Chicken(89%)
Clonality:
Polyclonal
Specificity:
Phospho-CFTR (Ser737) Antibody detects endogenous levels of CFTR only when phosphorylated at Ser737.
RRID:
AB_2840105
Cite Format: Affinity Biosciences Cat# AF8042, RRID:AB_2840105.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ABC 35; ABC35; ABCC 7; ABCC7; ATP binding cassette sub family C member 7; ATP Binding Cassette Superfamily C Member 7; ATP binding cassette transporter sub family C member 7; ATP-binding cassette sub-family C member 7; cAMP dependent chloride channel; cAMP-dependent chloride channel; CF; CFTR; CFTR/MRP; CFTR_HUMAN; Channel conductance controlling ATPase; Channel conductance-controlling ATPase; Cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub family C, member 7); Cystic fibrosis transmembrane conductance regulator; Cystic fibrosis transmembrane conductance regulator ATP binding cassette sub family C member 7; Cystic Fibrosis Transmembrane Regulator; dJ760C5.1; MRP 7; MRP7; TNR CFTR;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P13569 CFTR_HUMAN:

Expressed in the respiratory airway, including bronchial epithelium, and in the female reproductive tract, including oviduct (at protein level) (PubMed:22207244, PubMed:15716351). Detected in pancreatic intercalated ducts in the exocrine tissue, on epithelial cells in intralobular striated ducts in sublingual salivary glands, on apical membranes of crypt cells throughout the small and large intestine, and on the reabsorptive duct in eccrine sweat glands (PubMed:1284548, PubMed:28130590). Detected on the equatorial segment of the sperm head (at protein level) (PubMed:19923167). Detected in nasal and bronchial superficial epithelium (PubMed:15716351). Expressed by the central cells on the sebaceous glands, dermal adipocytes and, at lower levels, by epithelial cells (PubMed:28130590).

Sequence:
MQRSPLEKASVVSKLFFSWTRPILRKGYRQRLELSDIYQIPSVDSADNLSEKLEREWDRELASKKNPKLINALRRCFFWRFMFYGIFLYLGEVTKAVQPLLLGRIIASYDPDNKEERSIAIYLGIGLCLLFIVRTLLLHPAIFGLHHIGMQMRIAMFSLIYKKTLKLSSRVLDKISIGQLVSLLSNNLNKFDEGLALAHFVWIAPLQVALLMGLIWELLQASAFCGLGFLIVLALFQAGLGRMMMKYRDQRAGKISERLVITSEMIENIQSVKAYCWEEAMEKMIENLRQTELKLTRKAAYVRYFNSSAFFFSGFFVVFLSVLPYALIKGIILRKIFTTISFCIVLRMAVTRQFPWAVQTWYDSLGAINKIQDFLQKQEYKTLEYNLTTTEVVMENVTAFWEEGFGELFEKAKQNNNNRKTSNGDDSLFFSNFSLLGTPVLKDINFKIERGQLLAVAGSTGAGKTSLLMVIMGELEPSEGKIKHSGRISFCSQFSWIMPGTIKENIIFGVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEIFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLQPDFSSKLMGCDSFDQFSAERRNSILTETLHRFSLEGDAPVSWTETKKQSFKQTGEFGEKRKNSILNPINSIRKFSIVQKTPLQMNGIEEDSDEPLERRLSLVPDSEQGEAILPRISVISTGPTLQARRRQSVLNLMTHSVNQGQNIHRKTTASTRKVSLAPQANLTELDIYSRRLSQETGLEISEEINEEDLKECFFDDMESIPAVTTWNTYLRYITVHKSLIFVLIWCLVIFLAEVAASLVVLWLLGNTPLQDKGNSTHSRNNSYAVIITSTSSYYVFYIYVGVADTLLAMGFFRGLPLVHTLITVSKILHHKMLHSVLQAPMSTLNTLKAGGILNRFSKDIAILDDLLPLTIFDFIQLLLIVIGAIAVVAVLQPYIFVATVPVIVAFIMLRAYFLQTSQQLKQLESEGRSPIFTHLVTSLKGLWTLRAFGRQPYFETLFHKALNLHTANWFLYLSTLRWFQMRIEMIFVIFFIAVTFISILTTGEGEGRVGIILTLAMNIMSTLQWAVNSSIDVDSLMRSVSRVFKFIDMPTEGKPTKSTKPYKNGQLSKVMIIENSHVKKDDIWPSGGQMTVKDLTAKYTEGGNAILENISFSISPGQRVGLLGRTGSGKSTLLSAFLRLLNTEGEIQIDGVSWDSITLQQWRKAFGVIPQKVFIFSGTFRKNLDPYEQWSDQEIWKVADEVGLRSVIEQFPGKLDFVLVDGGCVLSHGHKQLMCLARSVLSKAKILLLDEPSAHLDPVTYQIIRRTLKQAFADCTVILCEHRIEAMLECQQFLVIEENKVRQYDSIQKLLNERSLFRQAISPSDRVKLFPHRNSSKCKSKPQIAALKEETEEEVQDTRL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
89
Chicken
89
Zebrafish
86
Xenopus
78
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13569 As Substrate

Site PTM Type Enzyme
Ubiquitination
S45 Phosphorylation
S50 Phosphorylation
T94 Phosphorylation
S256 Phosphorylation
T291 Phosphorylation
K420 Ubiquitination
S422 Phosphorylation P68400 (CSNK2A1)
K447 Sumoylation
S511 Phosphorylation P68400 (CSNK2A1)
Y512 Phosphorylation P43405 (SYK) , P07948 (LYN)
Y515 Phosphorylation P07948 (LYN)
Y517 Phosphorylation P07948 (LYN)
S549 Phosphorylation
T582 Phosphorylation
T604 Phosphorylation
S605 Phosphorylation
S641 Phosphorylation
S660 Phosphorylation P17612 (PRKACA) , P17252 (PRKCA) , Q13976 (PRKG1)
T665 Phosphorylation
S670 Phosphorylation
T682 Phosphorylation
S686 Phosphorylation
K688 Ubiquitination
S700 Phosphorylation P17612 (PRKACA)
S707 Phosphorylation
S712 Phosphorylation P17612 (PRKACA)
T717 Phosphorylation
S737 Phosphorylation Q13131 (PRKAA1) , P17252 (PRKCA) , Q8IWU2 (LMTK2) , Q13976 (PRKG1) , P17612 (PRKACA)
S753 Phosphorylation P17612 (PRKACA)
S768 Phosphorylation P17612 (PRKACA) , Q13131 (PRKAA1) , Q13976 (PRKG1)
T774 Phosphorylation
S776 Phosphorylation
S790 Phosphorylation
S795 Phosphorylation P17252 (PRKCA) , Q13976 (PRKG1) , P17612 (PRKACA)
S809 Phosphorylation
S813 Phosphorylation P17612 (PRKACA) , Q13131 (PRKAA1) , Q13976 (PRKG1) , P17252 (PRKCA)
N894 N-Glycosylation
N900 N-Glycosylation
Y1014 Phosphorylation
T1019 Phosphorylation
S1045 Phosphorylation
T1176 Phosphorylation
S1362 Phosphorylation
Y1424 Phosphorylation
S1444 Phosphorylation
S1455 Phosphorylation
S1456 Phosphorylation
T1471 Phosphorylation P68400 (CSNK2A1) , PR:000037188 (casein kinase II)

Research Backgrounds

Function:

Epithelial ion channel that plays an important role in the regulation of epithelial ion and water transport and fluid homeostasis. Mediates the transport of chloride ions across the cell membrane. Channel activity is coupled to ATP hydrolysis. The ion channel is also permeable to HCO(3-); selectivity depends on the extracellular chloride concentration. Exerts its function also by modulating the activity of other ion channels and transporters. Plays an important role in airway fluid homeostasis. Contributes to the regulation of the pH and the ion content of the airway surface fluid layer and thereby plays an important role in defense against pathogens. Modulates the activity of the epithelial sodium channel (ENaC) complex, in part by regulating the cell surface expression of the ENaC complex. Inhibits the activity of the ENaC channel containing subunits SCNN1A, SCNN1B and SCNN1G. Inhibits the activity of the ENaC channel containing subunits SCNN1D, SCNN1B and SCNN1G, but not of the ENaC channel containing subunits SCNN1A, SCNN1B and SCNN1G. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the transporter SLC4A7. Can inhibit the chloride channel activity of ANO1. Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation.

PTMs:

N-glycosylated.

Phosphorylated; cAMP treatment promotes phosphorylation and activates the channel. Dephosphorylation decreases the ATPase activity (in vitro). Phosphorylation at PKA sites activates the channel. Phosphorylation at PKC sites enhances the response to phosphorylation by PKA. Phosphorylated by AMPK; this inhibits channel activity.

Ubiquitinated, leading to its degradation in the lysosome. Deubiquitination by USP10 in early endosomes enhances its endocytic recycling to the cell membrane. Ubiquitinated by RNF185 during ER stress.

Subcellular Location:

Apical cell membrane>Multi-pass membrane protein. Early endosome membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein. Recycling endosome membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein. Nucleus.
Note: The channel is internalized from the cell surface into an endosomal recycling compartment, from where it is recycled to the cell membrane (PubMed:17462998, PubMed:19398555, PubMed:20008117). In the oviduct and bronchus, detected on the apical side of epithelial cells, but not associated with cilia (PubMed:22207244). In Sertoli cells, a processed product is detected in the nucleus (By similarity). ER stress induces GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane (PubMed:21884936).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the respiratory airway, including bronchial epithelium, and in the female reproductive tract, including oviduct (at protein level). Detected in pancreatic intercalated ducts in the exocrine tissue, on epithelial cells in intralobular striated ducts in sublingual salivary glands, on apical membranes of crypt cells throughout the small and large intestine, and on the reabsorptive duct in eccrine sweat glands. Detected on the equatorial segment of the sperm head (at protein level). Detected in nasal and bronchial superficial epithelium. Expressed by the central cells on the sebaceous glands, dermal adipocytes and, at lower levels, by epithelial cells.

Subunit Structure:

Monomer; does not require oligomerization for channel activity. May form oligomers in the membrane. Interacts with SLC26A3, SLC26A6 and SHANK2 (By similarity). Interacts with SLC9A3R1 and MYO6. Interacts (via C-terminus) with GOPC (via PDZ domain); this promotes CFTR internalization and thereby decreases channel activity. Interacts with SLC4A7 through SLC9A3R1. Found in a complex with MYO5B and RAB11A. Interacts with ANO1. Interacts with SLC26A8. Interacts with AHCYL1; the interaction increases CFTR activity (By similarity). Interacts with CSE1L. The core-glycosylated form interacts with GORASP2 (via PDZ GRASP-type 1 domain) in respone to ER stress.

Family&Domains:

Binds and hydrolyzes ATP via the two cytoplasmic ABC transporter nucleotide-binding domains (PubMed:15284228). The two ATP-binding domains interact with each other, forming a head-to-tail dimer (PubMed:17036051). Normal ATPase activity requires interaction between the two domains (PubMed:15284228). The first ABC transporter nucleotide-binding domain has no ATPase activity by itself (By similarity).

The PDZ-binding motif mediates interactions with GOPC and with the SLC4A7, SLC9A3R1/EBP50 complex.

The R region is intrinsically disordered (PubMed:10792060, PubMed:17660831). It mediates channel activation when it is phosphorylated, but not in the absence of phosphorylation (PubMed:10792060).

Belongs to the ABC transporter superfamily. ABCC family. CFTR transporter (TC 3.A.1.202) subfamily.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Membrane transport > ABC transporters.

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

· Organismal Systems > Digestive system > Gastric acid secretion.

· Organismal Systems > Digestive system > Pancreatic secretion.

References

1). CFTR potentiator ivacaftor protects against noise-induced hair cell loss by increasing Nrf2 and reducing oxidative stress. Biomedicine & Pharmacotherapy, 2023 (PubMed: 37657258) [IF=7.5]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 2. Treatment with ivacaftor prevents the decrease of CFTR and the increase of p-CFTR (S737) after noise exposure in OHCs. (A) Representative images show immunolabeling for CFTR (red) and p-CFTR (S737, green) in OHC cytosol and nuclei in the upper basal turn measured 3 h after noise exposure. The enlarged OHCs allow for better visualization of the punctate labeling for CFTR and p-CFTR (S373). These images are representative of 3 mice in each group. Phalloidin (white) and DAPI (blue) were used as counterstaining for visualization of OHC cytoskeletal structure and nuclei, respectively. DMSO (control, solvent of ivacaftor); IVA (ivacaftor). Scale bar = 10 μm. (B–E) Semi-quantification of immunolabeling in grayscale of CFTR and p-CFTR (S737) in the OHC cytosol and nuclei shows that treatment with ivacaftor significantly prevents noise-decreased CFTR and increased p-CFTR (S737). Data are presented as means + SD, n = 3, *p < 0.05, **p < 0.01. (F) Representative immunoblots reveal a decrease in total CFTR expression and increase in p-CFTR (S737) in whole cochlear homogenates 3 h after noise exposure. These blots are representative of 3 repetitions with two cochleae per sample. GADPH serves as loading control. (G–I) Quantification of CFTR and p-CFTR (S737) band densities shows a significant decrease in CFTR levels (G), an increase in p-CFTR (S737) levels (H), and an increase in the ratio of p-CFTR (S737) to CFTR (I) in the PTS noise group compared to controls. Data are presented as means + SD, n = 3, **p < 0.01.

Application: WB    Species: Mouse    Sample:

Fig. 2. Treatment with ivacaftor prevents the decrease of CFTR and the increase of p-CFTR (S737) after noise exposure in OHCs. (A) Representative images show immunolabeling for CFTR (red) and p-CFTR (S737, green) in OHC cytosol and nuclei in the upper basal turn measured 3 h after noise exposure. The enlarged OHCs allow for better visualization of the punctate labeling for CFTR and p-CFTR (S373). These images are representative of 3 mice in each group. Phalloidin (white) and DAPI (blue) were used as counterstaining for visualization of OHC cytoskeletal structure and nuclei, respectively. DMSO (control, solvent of ivacaftor); IVA (ivacaftor). Scale bar = 10 μm. (B–E) Semi-quantification of immunolabeling in grayscale of CFTR and p-CFTR (S737) in the OHC cytosol and nuclei shows that treatment with ivacaftor significantly prevents noise-decreased CFTR and increased p-CFTR (S737). Data are presented as means + SD, n = 3, *p < 0.05, **p < 0.01. (F) Representative immunoblots reveal a decrease in total CFTR expression and increase in p-CFTR (S737) in whole cochlear homogenates 3 h after noise exposure. These blots are representative of 3 repetitions with two cochleae per sample. GADPH serves as loading control. (G–I) Quantification of CFTR and p-CFTR (S737) band densities shows a significant decrease in CFTR levels (G), an increase in p-CFTR (S737) levels (H), and an increase in the ratio of p-CFTR (S737) to CFTR (I) in the PTS noise group compared to controls. Data are presented as means + SD, n = 3

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