Phospho-PAK4/5/6 (Ser474) Antibody - #AF8027
| Product: | Phospho-PAK4/5/6 (Ser474) Antibody |
| Catalog: | AF8027 |
| Description: | Rabbit polyclonal antibody to Phospho-PAK4/5/6 (Ser474) |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat, Monkey |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 64kd,72kDa; 64kD,81kD,75kD(Calculated). |
| Uniprot: | O96013 | Q9P286 | Q9NQU5 |
| RRID: | AB_2840090 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF8027, RRID:AB_2840090.
Fold/Unfold
KIAA1142; p21 activated kinase 4; p21 Cdc42/Rac1-actiated kinase 4; P21 protein (Cdc42/Rac) activated kinase 4; p21(CDKN1A) activated kinase 4; p21-activated kinase 4; PAK 4; PAK-4; Pak4; PAK4_HUMAN; Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs; Serine threonine kinase PAK 4; Serine/threonine protein kinase PAK 4; Serine/threonine protein kinase PAK4; Serine/threonine-protein kinase PAK 4; EC 2.7.11.1; KIAA1264; MGC26232; p21 activated kinase 7; p21 protein (Cdc42/Rac)-activated kinase 7; p21(CDKN1A) activated kinase 7; p21-activated kinase 5; p21-activated kinase 7; PAK 5; PAK 7; PAK-5; PAK-7; PAK5; PAK7; PAK7_HUMAN; Protein kinase PAK5; Serine/threonine protein kinase PAK 7; Serine/threonine-protein kinase PAK 7; CDKN1A activated kinase 6; EC 2.7.1.37; p21 activated protein kinase 6; p21 protein (Cdc42/Rac)-activated kinase 6; p21(CDKN1A) activated kinase 6; p21-activated kinase 6; p21activated kinase 6; PAK 5; PAK 6; PAK-5; PAK-6; PAK5; Pak6; PAK6_HUMAN; Serine threonine protein kinase PAK 6; Serine/threonine protein kinase PAK 6; Serine/threonine protein kinase PAK6; Serine/threonine-protein kinase PAK 6;
Immunogens
Highest expression in prostate, testis and colon.
Q9P286 PAK5_HUMAN:Predominantly expressed in brain.
Q9NQU5 PAK6_HUMAN:Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast.
- O96013 PAK4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFGKRKKRVEISAPSNFEHRVHTGFDQHEQKFTGLPRQWQSLIEESARRPKPLVDPACITSIQPGAPKTIVRGSKGAKDGALTLLLDEFENMSVTRSNSLRRDSPPPPARARQENGMPEEPATTARGGPGKAGSRGRFAGHSEAGGGSGDRRRAGPEKRPKSSREGSGGPQESSRDKRPLSGPDVGTPQPAGLASGAKLAAGRPFNTYPRADTDHPSRGAQGEPHDVAPNGPSAGGLAIPQSSSSSSRPPTRARGAPSPGVLGPHASEPQLAPPACTPAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLDNFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPAQRATAAELLKHPFLAKAGPPASIVPLMRQNRTR
- Q9P286 PAK5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFGKKKKKIEISGPSNFEHRVHTGFDPQEQKFTGLPQQWHSLLADTANRPKPMVDPSCITPIQLAPMKTIVRGNKPCKETSINGLLEDFDNISVTRSNSLRKESPPTPDQGASSHGPGHAEENGFITFSQYSSESDTTADYTTEKYREKSLYGDDLDPYYRGSHAAKQNGHVMKMKHGEAYYSEVKPLKSDFARFSADYHSHLDSLSKPSEYSDLKWEYQRASSSSPLDYSFQFTPSRTAGTSGCSKESLAYSESEWGPSLDDYDRRPKSSYLNQTSPQPTMRQRSRSGSGLQEPMMPFGASAFKTHPQGHSYNSYTYPRLSEPTMCIPKVDYDRAQMVLSPPLSGSDTYPRGPAKLPQSQSKSGYSSSSHQYPSGYHKATLYHHPSLQSSSQYISTASYLSSLSLSSSTYPPPSWGSSSDQQPSRVSHEQFRAALQLVVSPGDPREYLANFIKIGEGSTGIVCIATEKHTGKQVAVKKMDLRKQQRRELLFNEVVIMRDYHHDNVVDMYSSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIATVCLSVLRALSYLHNQGVIHRDIKSDSILLTSDGRIKLSDFGFCAQVSKEVPKRKSLVGTPYWMAPEVISRLPYGTEVDIWSLGIMVIEMIDGEPPYFNEPPLQAMRRIRDSLPPRVKDLHKVSSVLRGFLDLMLVREPSQRATAQELLGHPFLKLAGPPSCIVPLMRQYRHH
- Q9NQU5 PAK6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFRKKKKKRPEISAPQNFQHRVHTSFDPKEGKFVGLPPQWQNILDTLRRPKPVVDPSRITRVQLQPMKTVVRGSAMPVDGYISGLLNDIQKLSVISSNTLRGRSPTSRRRAQSLGLLGDEHWATDPDMYLQSPQSERTDPHGLYLSCNGGTPAGHKQMPWPEPQSPRVLPNGLAAKAQSLGPAEFQGASQRCLQLGACLQSSPPGASPPTGTNRHGMKAAKHGSEEARPQSCLVGSATGRPGGEGSPSPKTRESSLKRRLFRSMFLSTAATAPPSSSKPGPPPQSKPNSSFRPPQKDNPPSLVAKAQSLPSDQPVGTFSPLTTSDTSSPQKSLRTAPATGQLPGRSSPAGSPRTWHAQISTSNLYLPQDPTVAKGALAGEDTGVVTHEQFKAALRMVVDQGDPRLLLDSYVKIGEGSTGIVCLAREKHSGRQVAVKMMDLRKQQRRELLFNEVVIMRDYQHFNVVEMYKSYLVGEELWVLMEFLQGGALTDIVSQVRLNEEQIATVCEAVLQALAYLHAQGVIHRDIKSDSILLTLDGRVKLSDFGFCAQISKDVPKRKSLVGTPYWMAPEVISRSLYATEVDIWSLGIMVIEMVDGEPPYFSDSPVQAMKRLRDSPPPKLKNSHKVSPVLRDFLERMLVRDPQERATAQELLDHPFLLQTGLPECLVPLIQLYRKQTSTC
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O96013/Q9P286/Q9NQU5 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y159 | Phosphorylation | Uniprot | |
| K167 | Acetylation | Uniprot | |
| Y182 | Phosphorylation | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| S226 | Phosphorylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| S271 | Phosphorylation | Uniprot | |
| Y272 | Phosphorylation | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| T281 | Phosphorylation | Uniprot | |
| R285 | Methylation | Uniprot | |
| S286 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| T349 | Phosphorylation | Uniprot | |
| Y350 | Phosphorylation | Uniprot | |
| S573 | Phosphorylation | Q9P286 (PAK5) | Uniprot |
| S602 | Phosphorylation | Uniprot | |
| T606 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K31 | Ubiquitination | Uniprot | |
| T33 | Phosphorylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| K78 | Methylation | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Q15139 (PRKD1) | Uniprot |
| S104 | Phosphorylation | Uniprot | |
| R126 | Methylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S167 | Phosphorylation | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| T187 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| T207 | Phosphorylation | Uniprot | |
| Y208 | Phosphorylation | Uniprot | |
| R254 | Methylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| T277 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| K455 | Acetylation | Uniprot | |
| K467 | Acetylation | Uniprot | |
| S474 | Phosphorylation | O96013 (PAK4) | Uniprot |
| T478 | Phosphorylation | Uniprot | |
| Y480 | Phosphorylation | Uniprot | |
| S488 | Phosphorylation | Uniprot | |
| K540 | Ubiquitination | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T69 | Phosphorylation | Uniprot | |
| Y81 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | Uniprot | |
| T99 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| Y129 | Phosphorylation | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| Y144 | Phosphorylation | Uniprot | |
| S165 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| S179 | Phosphorylation | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| S308 | Phosphorylation | Uniprot | |
| T326 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| S328 | Phosphorylation | Uniprot | |
| T339 | Phosphorylation | Uniprot | |
| S346 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| S351 | Phosphorylation | Uniprot | |
| T354 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| Y365 | Phosphorylation | Uniprot | |
| T371 | Phosphorylation | Uniprot | |
| K528 | Ubiquitination | Uniprot | |
| S560 | Phosphorylation | Q9NQU5 (PAK6) | Uniprot |
| T564 | Phosphorylation | P52564 (MAP2K6) | Uniprot |
| Y566 | Phosphorylation | P52564 (MAP2K6) | Uniprot |
| S616 | Phosphorylation | Uniprot |
PTMs - O96013/Q9P286/Q9NQU5 As Enzyme
| Substrate | Site | Source |
|---|---|---|
| P15923 (TCF3) | S39 | Uniprot |
| P15976 (GATA1) | S161 | Uniprot |
| P15976 (GATA1) | S187 | Uniprot |
| Q9P286 (PAK5) | S573 | Uniprot |
| Substrate | Site | Source |
|---|---|---|
| O96013-1 (PAK4) | S99 | Uniprot |
| O96013-1 (PAK4) | S104 | Uniprot |
| O96013-3 (PAK4) | S105 | Uniprot |
| O96013-3 (PAK4) | S138 | Uniprot |
| O96013-1 (PAK4) | S148 | Uniprot |
| O96013-1 (PAK4) | S167 | Uniprot |
| O96013-1 (PAK4) | S181 | Uniprot |
| O96013-1 (PAK4) | T207 | Uniprot |
| O96013-1 (PAK4) | S258 | Uniprot |
| O96013-1 (PAK4) | S291 | Uniprot |
| O96013-3 (PAK4) | T325 | Uniprot |
| O96013 (PAK4) | S474 | Uniprot |
| O96013-1 (PAK4) | T478 | Uniprot |
| P03372 (ESR1) | S305 | Uniprot |
| P04637 (TP53) | S215 | Uniprot |
| P18084 (ITGB5) | S759 | Uniprot |
| P18084 (ITGB5) | S762 | Uniprot |
| P35222 (CTNNB1) | S675 | Uniprot |
| P49023 (PXN) | S272 | Uniprot |
| P62826 (RAN) | S135 | Uniprot |
| Q15796 (SMAD2) | S465 | Uniprot |
| Q92934 (BAD) | S75 | Uniprot |
| Q92974-2 (ARHGEF2) | S885 | Uniprot |
| Q92974 (ARHGEF2) | S886 | Uniprot |
| Q93045 (STMN2) | S50 | Uniprot |
Research Backgrounds
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.
Autophosphorylated on serine residues when activated by CDC42/p21 (Ref.31). Phosphorylated on tyrosine residues upon stimulation of FGFR2 (By similarity).
Polyubiquitinated, leading to its proteasomal degradation.
Cytoplasm.
Note: Seems to shuttle between cytoplasmic compartments depending on the activating effector. For example, can be found on the cell periphery after activation of growth-factor or integrin-mediated signaling pathways.
Highest expression in prostate, testis and colon.
Interacts with FGFR2 and GRB2 (By similarity). Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and weakly with RAC1. Interacts with INKA1. Interacts with SH3RF2.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions.
Autophosphorylated when activated by CDC42/p21.
Mitochondrion. Cytoplasm. Nucleus.
Note: Shuttles between the nucleus and the mitochondria, and mitochondrial localization is essential for the role in cell survival.
Predominantly expressed in brain.
Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Interacts with MARK2, leading to inhibit MARK2 independently of kinase activity. Interacts with RHOD and RHOH.
An autoinhibitory domain is present in the N-terminal region of the protein.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Inhibits also ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD.
Autophosphorylated. Phosphorylated by MAP2K6//MAPKK6, leading to PAK6 activation.
Cytoplasm. Nucleus.
Note: Cotranslocates into nucleus with AR in response to androgen induction.
Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast.
Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1 (By similarity). Interacts with the androgen receptor AR and the estrogen receptor ESR1. Interacts with IQGAP1 and PPM1B.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Research Fields
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
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