Phospho-HSL (Ser660) Antibody | Affinity Biosciences

IHC
pElisa
Cites

1/8

AF8026 at 1/200 staining Human kidney tissue sections by IHC-P.

AF8026 at 1/200 staining Mouse kidney tissue sections by IHC-P.

AF8026 at 1/200 staining Rat brain tissue sections by IHC-P.

AF8026 at 1/100 staining Rat spleen tissue by IHC-P.

peptide-ELISA analysis of AF8026.

Fig.

Product:	Phospho-HSL (Ser660) Antibody
Catalog:	AF8026
Description:	Rabbit polyclonal antibody to Phospho-HSL (Ser660)
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Dog
Mol.Wt.:	81/83kDa; 117kD(Calculated).
Uniprot:	Q05469
RRID:	AB_2840089

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$450	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(88%), Horse(89%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Phospho-HSL (Ser660) Antibody detects endogenous levels of HSL only when phosphorylated at Ser660.

RRID:

AB_2840089
Cite Format: Affinity Biosciences Cat# AF8026, RRID:AB_2840089.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Hormone sensitive lipase; Hormone sensitive lipase testicular isoform; Hormone-sensitive lipase; HSL; LHS; Lipase hormone sensitive; LIPE; LIPS_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q05469(LIPS_HUMAN) >>Visit HPA database.

Gene(ID):

LIPE(3991)

Sequence:

Q05469 LIPS_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEPGSKSVSRSDWQPEPHQRPITPLEPGPEKTPIAQPESKTLQGSNTQQKPASNQRPLTQQETPAQHDAESQKEPRAQQKSASQEEFLAPQKPAPQQSPYIQRVLLTQQEAASQQGPGLGKESITQQEPALRQRHVAQPGPGPGEPPPAQQEAESTPAAQAKPGAKREPSAPTESTSQETPEQSDKQTTPVQGAKSKQGSLTELGFLTKLQELSIQRSALEWKALSEWVTDSESESDVGSSSDTDSPATMGGMVAQGVKLGFKGKSGYKVMSGYSGTSPHEKTSARNHRHYQDTASRLIHNMDLRTMTQSLVTLAEDNIAFFSSQGPGETAQRLSGVFAGVREQALGLEPALGRLLGVAHLFDLDPETPANGYRSLVHTARCCLAHLLHKSRYVASNRRSIFFRTSHNLAELEAYLAALTQLRALVYYAQRLLVTNRPGVLFFEGDEGLTADFLREYVTLHKGCFYGRCLGFQFTPAIRPFLQTISIGLVSFGEHYKRNETGLSVAASSLFTSGRFAIDPELRGAEFERITQNLDVHFWKAFWNITEMEVLSSLANMASATVRVSRLLSLPPEAFEMPLTADPTLTVTISPPLAHTGPGPVLVRLISYDLREGQDSEELSSLIKSNGQRSLELWPRPQQAPRSRSLIVHFHGGGFVAQTSRSHEPYLKSWAQELGAPIISIDYSLAPEAPFPRALEECFFAYCWAIKHCALLGSTGERICLAGDSAGGNLCFTVALRAAAYGVRVPDGIMAAYPATMLQPAASPSRLLSLMDPLLPLSVLSKCVSAYAGAKTEDHSNSDQKALGMMGLVRRDTALLLRDFRLGASSWLNSFLELSGRKSQKMSEPIAEPMRRSVSEAALAQPQGPLGTDSLKNLTLRDLSLRGNSETSSDTPEMSLSAETLSPSTPSDVNFLLPPEDAGEEAEAKNELSPMDRGLGVRAAFPEGFHPRRSSQGATQMPLYSSPIVKNPFMSPLLAPDSMLKSLPPVHIVACALDPMLDDSVMLARRLRNLGQPVTLRVVEDLPHGFLTLAALCRETRQAAELCVERIRLVLTPPAGAGPSGETGAAGVDGGCGGRH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Dog

100

Horse

Bovine

Rabbit

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q05469 As Substrate

Q05469

Site	PTM Type	Enzyme	Source
T188	Phosphorylation		Uniprot
S196	Phosphorylation		Uniprot
S552	Phosphorylation		Uniprot
S565	Phosphorylation		Uniprot
K624	Ubiquitination		Uniprot
S630	Phosphorylation		Uniprot
S645	Phosphorylation		Uniprot
S649	Phosphorylation		Uniprot
S650	Phosphorylation		Uniprot
S660	Phosphorylation		Uniprot
Y741	Phosphorylation		Uniprot
T813	Phosphorylation		Uniprot
S825	Phosphorylation		Uniprot
S835	Phosphorylation		Uniprot
S853	Phosphorylation	P17612 (PRKACA) , Q13976 (PRKG1)	Uniprot
S855	Phosphorylation	Q14012 (CAMK1) , P17612 (PRKACA) , Q13131 (PRKAA1) , Q9UQM7 (CAMK2A)	Uniprot
K872	Ubiquitination		Uniprot
S950	Phosphorylation	P17612 (PRKACA)	Uniprot
S951	Phosphorylation	P17612 (PRKACA)	Uniprot
T955	Phosphorylation		Uniprot
T1015	Phosphorylation		Uniprot

Research Backgrounds

Q05469_LIPS_HUMAN

Function:

In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.

PTMs:

Phosphorylation by AMPK may block translocation to lipid droplets.

Subcellular Location:

Cell membrane. Membrane>Caveola. Cytoplasm>Cytosol.
Note: Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.

Subunit Structure:

Interacts with CAVIN1 in the adipocyte cytoplasm. Interacts with PLIN5 (By similarity).

Family&Domains:

Belongs to the 'GDXG' lipolytic enzyme family.

Research Fields

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

References

1). Lipopolysaccharide binding protein resists hepatic oxidative stress by regulating lipid droplet homeostasis. Nature communications, 2024 (PubMed: 38615060) [IF=16.6]

2). Effect of salt promote the muscle triglyceride hydrolysis during dry-salting by inducing the phosphorylation of adipose tissue triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) and lipid droplets splitting. Food Chemistry, 2020 (PubMed: 32454271) [IF=8.8]

Application: WB Species: Pig Sample: adipose

Fig. 3. Western blotting analysis of ATGL, HSL and their phosphorylated protein contents in adipose tissue during processing of dry-cured meat. (A) WB image of each dry-cured group. (B) ATGL relative protein level in adipose tissue. (C) p-ATGL(S406) relative protein level in adipose tissue. (D) HSL relative protein level in adipose tissue. (E) p-HSL(Ser563) relative protein level in adipose tissue. (F) p-HSL(Ser660) relative protein level in adipose tissue.

3). MIIP downregulation drives colorectal cancer progression through inducing peri-cancerous adipose tissue browning. Cell & bioscience, 2024 (PubMed: 38245780) [IF=7.5]

4). Melatonin reduces intramuscular fat deposition by promoting lipolysis and increasing mitochondrial function. JOURNAL OF LIPID RESEARCH, 2019 (PubMed: 30552289) [IF=6.5]

Application: WB Species: pig Sample: porcine intramuscular preadipocytes

Fig.?5.|Melatonin-activated PKA and ERK1/2 mediate lipolysis in porcine intramuscular preadipocytes. A–J: Fully differentiated adipocytes were treated with control, 1 mM of melatonin, and 1 mM of melatonin plus 10 M of 4-P-PDOT for 24 h. The expression levels of PKA (A, B), ERK1/2 (C, D), HSL (E, F), PLIN1 (G, H), and ATGL (I, J) and the phosphorylation levels of PKA (p-PKA Thr197) (A, B), ERK1/2 (p-ERK1/2 Thr202/Tyr204) (C, D), HSL (pHSL Ser660) (E, F), PLIN1 (p-PLIN1 Ser522) (G, H), and -tubulin (I, J) were evaluated by Western blotting. The results are represented as the mean ± SEM (*P < 0.05; **P < 0.01; ***P < 0.001; n = 3).

5). Sesamol Alleviates Obesity-Related Hepatic Steatosis via Activating Hepatic PKA Pathway. Nutrients, 2020 (PubMed: 31991934) [IF=5.9]

6). Effective fraction from Simiao Wan prevents hepatic insulin resistant by inhibition of lipolysis via AMPK activation. Chinese Journal of Natural Medicines, 2022 (PubMed: 35369960) [IF=4.6]

7). Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism. Food & Nutrition Research, 2022 (PubMed: 35382382) [IF=3.3]

Application: WB Species: mouse Sample: skeletal muscle

Fig. 6. | Effects of sesamol intervention on the expressions of glucose and lipid metabolism-related proteins in skeletal muscle of the middle-aged mice.(e) Representative western blot images and quantification of (f) SREBP1-c, (g) p-HSL and HSL and (h) CPT1α. *P < 0.05,**P < 0.01.

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Phospho-HSL (Ser660) Antibody - #AF8026