Phospho-Doublecortin (Ser297) Antibody

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Product:	Phospho-Doublecortin (Ser297) Antibody
Catalog:	AF8025
Description:	Rabbit polyclonal antibody to Phospho-Doublecortin (Ser297)
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	49.3kDa; 41kD(Calculated).
Uniprot:	O43602
RRID:	AB_2840088

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(89%), Bovine(89%), Horse(89%), Sheep(89%), Rabbit(89%), Dog(89%)

Clonality:

Polyclonal

Specificity:

Phospho-Doublecortin (Ser297) Antibody detects endogenous levels of Doublecortin only when phosphorylated at Ser297.

RRID:

AB_2840088
Cite Format: Affinity Biosciences Cat# AF8025, RRID:AB_2840088.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DBCN; Dbct; DC; DCX; DCX_HUMAN; Doublecortex; Doublin; FLJ51296; Lis X; Lis-X; Lissencephalin X; Lissencephalin-X; Lissencephaly X linked; Lissencephaly X linked doublecortin; LISX; Neuronal migration protein doublecortin; OTTHUMP00000023859; OTTHUMP00000023860; OTTHUMP00000216315; OTTHUMP00000216316; SCLH; XLIS;

Immunogens

Immunogen:

Uniprot:

O43602(DCX_HUMAN) >>Visit HPA database.

Gene(ID):

DCX(1641)

Expression:

O43602 DCX_HUMAN:

Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.

Sequence:

O43602 DCX_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MELDFGHFDERDKTSRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQVLTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSGNDQDANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKDLYLPLSLDDSDSLGDSM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Rabbit

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43602 As Substrate

O43602

Site	PTM Type	Enzyme	Source
S28	Phosphorylation		Uniprot
T30	Phosphorylation		Uniprot
S47	Phosphorylation		Uniprot
K50	Acetylation		Uniprot
K51	Acetylation		Uniprot
Y64	Phosphorylation		Uniprot
S74	Phosphorylation		Uniprot
Y103	Phosphorylation		Uniprot
Y105	Phosphorylation		Uniprot
S128	Phosphorylation	P17612 (PRKACA)	Uniprot
T203	Phosphorylation		Uniprot
S206	Phosphorylation		Uniprot
T222	Phosphorylation		Uniprot
S287	Phosphorylation		Uniprot
S297	Phosphorylation		Uniprot
S306	Phosphorylation		Uniprot
S320	Phosphorylation		Uniprot
S325	Phosphorylation		Uniprot
S329	Phosphorylation		Uniprot
S332	Phosphorylation	P49841 (GSK3B)	Uniprot
S335	Phosphorylation		Uniprot
S339	Phosphorylation		Uniprot
K345	Acetylation		Uniprot
K347	Acetylation		Uniprot

Research Backgrounds

O43602_DCX_HUMAN

Function:

Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.

PTMs:

Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules (By similarity). Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21 (By similarity).

Ubiquitinated by MDM2, leading to its degradation by the proteasome. Ubiquitinated by MDM2 and subsequent degradation leads to reduce the dendritic spine density of olfactory bulb granule cells.

Subcellular Location:

Cytoplasm. Cell projection>Neuron projection.
Note: Localizes at neurite tips.

Tissue Specificity:

Subunit Structure:

Interacts with tubulin. Interacts with USP9X.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-Doublecortin (Ser297) Antibody - #AF8025