MAST2 Antibody - #AF0571

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Product: MAST2 Antibody
Catalog: AF0571
Description: Rabbit polyclonal antibody to MAST2
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 196kDa; 196kD(Calculated).
Uniprot: Q6P0Q8
RRID: AB_2834288

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(91%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
MAST2 Antibody detects endogenous levels of total MAST2.
RRID:
AB_2834288
Cite Format: Affinity Biosciences Cat# AF0571, RRID:AB_2834288.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EC 2.7.11.1 3; FLJ39200; KIAA0807; MAST 2; MAST 205; Mast2; MAST2_HUMAN; Microtubule associated serine/threonine kinase 2; Microtubule associated testis specific serine/threonine protein kinase; Microtubule-associated serine/threonine-protein kinase 2; MTSSK; OTTHUMP00000009697 2; OTTHUMP00000046616; RP4 533D7.1; RP4-533D7.1 2;

Immunogens

Immunogen:
Uniprot:

Q6P0Q8(MAST2_HUMAN) >>Visit HPA database.

Gene(ID):
MAST2(23139)
Expression:
Q6P0Q8 MAST2_HUMAN:

Abundant in the testis.

Description:
MAST2 appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins. Functions in a multi-protein complex in spermatid maturation. Regulates lipopolysaccharide-induced IL-12 synthesis in macrophages by forming a complex with TRAF6, resulting in the inhibition of TRAF6 NF-kappaB activation. 2 isoforms of the human protein are produced by alternative splicing.
Sequence:
MKRSRCRDRPQPPPPDRREDGVQRAAELSQSLPPRRRAPPGRQRLEERTGPAGPEGKEQDVVTGVSPLLFRKLSNPDIFSSTGKVKLQRQLSQDDCKLWRGNLASSLSGKQLLPLSSSVHSSVGQVTWQSSGEASNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTESVPDEEGRQSPAMRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDSVLPLADGALSFIHHQVIEMARDCLDKSRSGLITSQYFYELQDNLEKLLQDAHERSESSEVAFVMQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSCDSPDTPETDDSIEGHGASLPSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFDTKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDEALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHMDSEDEEEVSEDGCLEIRQFSSCSPRFNKVYSSMERLSLLEERRTPPPTKRSLSEEKEDHSDGLAGLKGRDRSWVIGSPEILRKRLSVSESSHTESDSSPPMTVRRRCSGLLDAPRFPEGPEEASSTLRRQPQEGIWVLTPPSGEGVSGPVTEHSGEQRPKLDEEAVGRSSGSSPAMETRGRGTSQLAEGATAKAISDLAVRRARHRLLSGDSTEKRTARPVNKVIKSASATALSLLIPSEHHTCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALGSMRPPIIIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVAISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPDAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPPTASPQRSPSPLSGHVAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKHSLDLPHSELKKELPPREVSPLEVVGARSVLSGKGALPGKGVLQPAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTEEGPENSQGAQELSLAPHPEVSQSVAPKGAGESGEEDPFPSRDPRSLGPMVPSLLTGITLGPPRMESPSGPHRRLGSPQAIEEAASSSSAGPNLGQSGATDPIPPEGCWKAQHLHTQALTALSPSTSGLTPTSSCSPPSSTSGKLSMWSWKSLIEGPDRASPSRKATMAGGLANLQDLENTTPAQPKNLSPREQGKTQPPSAPRLAHPSYEDPSQGWLWESECAQAVKEDPALSITQVPDASGDRRQDVPCRGCPLTQKSEPSLRRGQEPGGHQKHRDLALVPDELLKQT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Pig
91
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6P0Q8 As Substrate

Site PTM Type Enzyme
T63 Phosphorylation
S66 Phosphorylation
S74 Phosphorylation
S81 Phosphorylation
T82 Phosphorylation
S92 Phosphorylation
S108 Phosphorylation
S144 Phosphorylation
S148 Phosphorylation
S151 Phosphorylation
S160 Phosphorylation
R163 Methylation
R169 Methylation
S183 Phosphorylation
T185 Phosphorylation
S191 Phosphorylation
S204 Phosphorylation
S209 Phosphorylation
S230 Phosphorylation
T243 Phosphorylation
T274 Phosphorylation
S278 Phosphorylation
T279 Phosphorylation
S281 Phosphorylation
S290 Phosphorylation
S297 Phosphorylation
S299 Phosphorylation
S301 Phosphorylation
S305 Phosphorylation
K455 Ubiquitination
Y461 Phosphorylation
S519 Phosphorylation
S533 Phosphorylation
T534 Phosphorylation
K545 Ubiquitination
S657 Phosphorylation
K683 Ubiquitination
K797 Ubiquitination
S806 Phosphorylation
Y812 Phosphorylation
S825 Phosphorylation
S846 Phosphorylation
Y853 Phosphorylation
S854 Phosphorylation
S855 Phosphorylation
S860 Phosphorylation
T867 Phosphorylation
T871 Phosphorylation
S874 Phosphorylation
S876 Phosphorylation
S883 Phosphorylation
K890 Ubiquitination
S895 Phosphorylation
S900 Phosphorylation
S920 Phosphorylation
S931 Phosphorylation
S993 Phosphorylation
S995 Phosphorylation
S996 Phosphorylation
R1004 Methylation
S1007 Phosphorylation
S1032 Phosphorylation
S1035 Phosphorylation
S1180 Phosphorylation
K1183 Ubiquitination
S1195 Phosphorylation
K1197 Ubiquitination
K1203 Acetylation
K1207 Acetylation
K1209 Acetylation
K1237 Ubiquitination
S1240 Phosphorylation
S1245 Phosphorylation
S1247 Phosphorylation
S1250 Phosphorylation
S1256 Phosphorylation
S1264 Phosphorylation
S1275 Phosphorylation
S1337 Phosphorylation P28482 (MAPK1)
S1340 Phosphorylation
S1344 Phosphorylation
T1349 Phosphorylation
S1351 Phosphorylation
S1364 Phosphorylation
S1381 Phosphorylation
S1393 Phosphorylation
S1399 Phosphorylation
K1403 Methylation
S1407 Phosphorylation
S1425 Phosphorylation
S1429 Phosphorylation
S1435 Phosphorylation
S1447 Phosphorylation
S1503 Phosphorylation
S1504 Phosphorylation
T1508 Phosphorylation
S1541 Phosphorylation
S1554 Phosphorylation
T1564 Phosphorylation
T1567 Phosphorylation
S1585 Phosphorylation
S1631 Phosphorylation
S1642 Phosphorylation
K1659 Methylation
S1660 Phosphorylation
S1669 Phosphorylation
S1698 Phosphorylation
S1717 Phosphorylation
S1768 Phosphorylation
S1771 Phosphorylation
T1798 Phosphorylation

Research Backgrounds

Function:

Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins. Functions in a multi-protein complex in spermatid maturation. Regulates lipopolysaccharide-induced IL-12 synthesis in macrophages by forming a complex with TRAF6, resulting in the inhibition of TRAF6 NF-kappa-B activation (By similarity).

PTMs:

Phosphorylated and ubiquitinated. N-terminal ubiquitination leads to degradation of MAST2 by proteasome-mediated proteolysis. N-terminal phosphorylation appears to be a prerequisite for ubiquitination (By similarity).

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Recruited to the sub-membranous area on interaction with CDHR2.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Abundant in the testis.

Subunit Structure:

Interacts with CDHR2.

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

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