Phospho-MRLC1 (Thr19+Ser20) Antibody - #AF8010

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Product: Phospho-MRLC1 (Thr19+Ser20) Antibody
Catalog: AF8010
Description: Rabbit polyclonal antibody to Phospho-MRLC1 (Thr19+Ser20)
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 18kDa; 20kD(Calculated).
Uniprot: P24844
RRID: AB_2840073

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 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-MRLC1 (Thr19+Ser20) Antibody detects endogenous levels of MRLC1 only when phosphorylated at Thr19+Ser20.
RRID:
AB_2840073
Cite Format: Affinity Biosciences Cat# AF8010, RRID:AB_2840073.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

20 kDa myosin light chain;Human 20kDa myosin light chain (MLC2) mRNA complete cds;LC20;MGC3505;MLC 2;MLC-2C;MLC2;MLY 9;MRLC1;MYL9;MYL9_HUMAN;Myosin light chain 9 regulatory;Myosin light polypeptide 9 regulatory;myosin regulatory light chain 1;Myosin regulatory light chain 2;Myosin regulatory light chain 2 smooth muscle isoform;Myosin regulatory light chain 9;Myosin regulatory light chain MRLC1;Myosin regulatory light polypeptide 9;Myosin RLC;Myosin vascular smooth muscle light chain 2;MYRL2;OTTHUMP00000030857;smooth muscle isoform; ML12B_HUMAN;MLC 2A;MLC-2;MLC-2a;MLC-B;MLC20;MRLC2;MYL12B;MYLC2B;Myosin light chain 12B regulatory;Myosin regulatory light chain 12B;myosin regulatory light chain 2;Myosin regulatory light chain 2-B;myosin regulatory light chain 2-B, smooth muscle isoform;Myosin regulatory light chain 20 kDa;Myosin regulatory light chain MRLC2;myosin, light chain 12B, regulatory;OTTHUMP00000162244;OTTHUMP00000165806;OTTHUMP00000165807;OTTHUMP00000165808;SHUJUN-1;smooth muscle isoform;

Immunogens

Immunogen:
Uniprot:

P24844(MYL9_HUMAN) >>Visit HPA database.

Gene(ID):
MYL9(10398)
Expression:
P24844 MYL9_HUMAN:

Smooth muscle tissues and in some, but not all, nonmuscle cells.

Description:
Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3). Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity (4,5). Phosphorylation by cdc2 controls the timing of cytokinesis (5). Transgenic mice lacking phosphorylation sites on the cardiac muscle isoform show morphological and functional abnormalities (6).
Sequence:
MSSKRAKAKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKDD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P24844 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation P17252 (PRKCA)
S3 Phosphorylation P17252 (PRKCA)
T10 Phosphorylation P17252 (PRKCA)
T11 Phosphorylation
T19 Phosphorylation O43293 (DAPK3) , O14578 (CIT) , Q15746 (MYLK) , Q5VT25 (CDC42BPA) , Q13464 (ROCK1)
S20 Phosphorylation O43293 (DAPK3) , Q5VT25 (CDC42BPA) , O75116 (ROCK2) , Q14012 (CAMK1) , Q15746 (MYLK) , Q13153 (PAK1) , Q13177 (PAK2) , Q9Y5S2 (CDC42BPB) , O14578 (CIT) , Q13464 (ROCK1)
S29 Phosphorylation
K51 Acetylation
K51 Ubiquitination
S60 Phosphorylation
T97 Phosphorylation
S115 Phosphorylation
T128 Phosphorylation
T135 Phosphorylation
Y143 Phosphorylation P00533 (EGFR)
K150 Ubiquitination
K151 Sumoylation
K151 Ubiquitination
Y156 Phosphorylation P00533 (EGFR)
K164 Ubiquitination
K168 Acetylation

Research Backgrounds

Function:

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.

PTMs:

Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.

Tissue Specificity:

Smooth muscle tissues and in some, but not all, nonmuscle cells.

Subunit Structure:

Myosin is a hexamer of 2 heavy chains and 4 light chains: interacts with myosin heavy chain MYO19.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

References

1). Patchouli alcohol attenuates 5-fluorouracil-induced intestinal mucositis via TLR2/MyD88/NF-kB pathway and regulation of microbiota. BIOMEDICINE & PHARMACOTHERAPY, 2020 (PubMed: 32004938) [IF=7.5]

Application: WB    Species: rat    Sample: Intestinal

Fig. 4. |Effect of PA on intestinal mocusal barrier proteins. (a–d) Relative mRNA expression of MLCK, ZO-1, occludin and claudin-1 (n = 4); (e–i) Expressions of MLC,p-MLC, ZO-1, occludin and claudin-1proteins (n = 3). Values were represented the mean ± SEM. **P < 0.01, *P < 0.05 versus 5-FU group and ##P < 0.01 versus normal group.
2). Study on the Mechanism of Sancao Tiaowei Decoction in the Treatment of MNNG-Induced Precancerous Lesions of Gastric Carcinoma Through Hedgehog Signaling Pathway. Frontiers in Oncology, 2022 (PubMed: 35646631) [IF=4.7]
3). High Glucose Aggravates Retinal Endothelial Cell Dysfunction by Activating the RhoA/ROCK1/pMLC/Connexin43 Signaling Pathway. Investigative Ophthalmology & Visual Science, 2022 (PubMed: 35881407) [IF=4.4]

Application: WB    Species: Mice    Sample: mRMVECs

Figure 6.RhoA/ROCK1 signaling regulates Cx43 through the directly interaction between pMLC and Cx43. (A, C) Co-immunoprecipitation of pMLC and Cx43 in mRMVECs with or without high glucose. (B, D, E) The protein expression of pMLC, MLC, and Cx43 in mRMVECs treated with different conditions. ns, no significance. EV, empty vector. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared with the control group; ##P < 0.01 compared with the high glucose (30 mM) group. N = 3. The bars are the mean ± SD.

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