STEA3 Antibody | Affinity Biosciences

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Western blot analysis on COLO205 cell lysates using STEA3 Antibody,The lane on the left was treated with the antigen-specific peptide.

AF0652 at 1/100 staining mouse liver tissue by IHC-P.

Product:	STEA3 Antibody
Catalog:	AF0652
Description:	Rabbit polyclonal antibody to STEA3
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	54kDa; 55kD(Calculated).
Uniprot:	Q658P3
RRID:	AB_2834284

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(90%), Chicken(90%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

STEA3 Antibody detects endogenous levels of total STEA3.

RRID:

AB_2834284
Cite Format: Affinity Biosciences Cat# AF0652, RRID:AB_2834284.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

1010001D01Rik; Dudlin 2; Dudlin2; Dudulin 2; Dudulin-2; Dudulin2; hpHyde; hTSAP6; Metalloreductase STEAP3; MGC93147; pHyde; Six transmembrane epithelial antigen of prostate 3; Six transmembrane prostate protein 3; Six-transmembrane epithelial antigen of prostate 3; STEA 3; STEA3_HUMAN; STEAP 3; STEAP family member 3; STEAP family member 3 metalloreductase; STEAP3; STMP 3; STMP3; TSAP 6; TSAP6; Tumor suppressor activated pathway protein 6; Tumor suppressor pHyde; Tumor suppressor-activated pathway protein 6;

Immunogens

Immunogen:

Uniprot:

Q658P3(STEA3_HUMAN) >>Visit HPA database.

Gene(ID):

STEAP3(55240)

Expression:

Q658P3 STEA3_HUMAN:

Expressed in adult bone marrow, placenta, liver, skeletal muscle and pancreas. Down-regulated in hepatocellular carcinoma.

Description:

STEAP3 a multi-pass membrane protein induced directly by p53. Plays an important role in facilitating exosome secretion. Enhances secretion in the DNA damage-induced p53-dependent exosomal secretory pathway. Localizes to vesicular-like structures at the plasma membrane and around the nucleus. Highly expressed in hematopoietic tissues where it localizes to the specialized endosome. An endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NAD(+) as acceptor.

Sequence:

Q658P3 STEA3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPEEMDKPLISLHLVDSDSSLAKVPDEAPKVGILGSGDFARSLATRLVGSGFKVVVGSRNPKRTARLFPSAAQVTFQEEAVSSPEVIFVAVFREHYSSLCSLSDQLAGKILVDVSNPTEQEHLQHRESNAEYLASLFPTCTVVKAFNVISAWTLQAGPRDGNRQVPICGDQPEAKRAVSEMALAMGFMPVDMGSLASAWEVEAMPLRLLPAWKVPTLLALGLFVCFYAYNFVRDVLQPYVQESQNKFFKLPVSVVNTTLPCVAYVLLSLVYLPGVLAAALQLRRGTKYQRFPDWLDHWLQHRKQIGLLSFFCAALHALYSFCLPLRRAHRYDLVNLAVKQVLANKSHLWVEEEVWRMEIYLSLGVLALGTLSLLAVTSLPSIANSLNWREFSFVQSSLGFVALVLSTLHTLTYGWTRAFEESRYKFYLPPTFTLTLLVPCVVILAKALFLLPCISRRLARIRRGWERESTIKFTLPTDHALAEKTSHV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Xenopus

100

Rabbit

100

Dog

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q658P3 As Substrate

Q658P3

Site	PTM Type	Source
K7	Ubiquitination	Uniprot
S11	Phosphorylation	Uniprot
S17	Phosphorylation	Uniprot
S19	Phosphorylation	Uniprot
S20	Phosphorylation	Uniprot
K23	Ubiquitination	Uniprot
K30	Ubiquitination	Uniprot
S42	Phosphorylation	Uniprot
T141	Phosphorylation	Uniprot
Y239	Phosphorylation	Uniprot
S243	Phosphorylation	Uniprot
N256	N-Glycosylation	Uniprot
Y288	Phosphorylation	Uniprot
Y331	Phosphorylation	Uniprot
K339	Ubiquitination	Uniprot
N344	N-Glycosylation	Uniprot
S469	Phosphorylation	Uniprot
T470	Phosphorylation	Uniprot
K472	Ubiquitination	Uniprot
T474	Phosphorylation	Uniprot
T477	Phosphorylation	Uniprot
K484	Ubiquitination	Uniprot
S486	Phosphorylation	Uniprot

Research Backgrounds

Q658P3_STEA3_HUMAN

Function:

Endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NADP(+) as acceptor. May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP.

PTMs:

Proteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage occurs at multiple sites in a glycosylation-independent manner.

Glycosylated.

Subcellular Location:

Endosome membrane>Multi-pass membrane protein.
Note: Localizes to vesicular-like structures at the plasma membrane and around the nucleus.

Tissue Specificity:

Expressed in adult bone marrow, placenta, liver, skeletal muscle and pancreas. Down-regulated in hepatocellular carcinoma.

Subunit Structure:

Homodimer. Interacts with BNIP3L, MYT1, RHBDL4/RHBDD1 and TCTP.

Family&Domains:

Belongs to the STEAP family.

Research Fields

· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)

· Cellular Processes > Cell growth and death > Ferroptosis. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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STEA3 Antibody - #AF0652