DOK7 Antibody | Affinity Biosciences

WB
IHC
Cites

1/3

Western blot analysis of extracts from A2780 cells, using DOK7 Antibody.

AF0681 at 1/100 staining human Heart muscle tissue sections by IHC-P.

Figure 4:| OLA attenuates mTORC2 complex activity.

Product:	DOK7 Antibody
Catalog:	AF0681
Description:	Rabbit polyclonal antibody to DOK7
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Horse
Mol.Wt.:	60kDa; 53kD(Calculated).
Uniprot:	Q18PE1
RRID:	AB_2834274

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Horse(100%)

Clonality:

Polyclonal

Specificity:

DOK7 Antibody detects endogenous levels of total DOK7.

RRID:

AB_2834274
Cite Format: Affinity Biosciences Cat# AF0681, RRID:AB_2834274.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Docking protein 7; DOK 7; DOK7; DOK7_HUMAN; Downstream of tyrosine kinase 7; Protein Dok-7;

Immunogens

Immunogen:

Uniprot:

Q18PE1(DOK7_HUMAN) >>Visit HPA database.

Gene(ID):

DOK7(285489)

Expression:

Q18PE1 DOK7_HUMAN:

Preferentially expressed in skeletal muscle and heart. Present in thigh muscle, diaphragm and heart but not in the liver or spleen (at protein level).

Description:

Dok7 docking proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK.

Sequence:

Q18PE1 DOK7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTEAALVEGQVKLRDGKKWKSRWLVLRKPSPVADCLLMLVYKDKSERIKGLRERSSLTLEDICGLEPGLPYEGLVHTLAIVCLSQAIMLGFDSHEAMCAWDARIRYALGEVHRFHVTVAPGTKLESGPATLHLCNDVLVLARDIPPAVTGQWKLSDLRRYGAVPSGFIFEGGTRCGYWAGVFFLSSAEGEQISFLFDCIVRGISPTKGPFGLRPVLPDPSPPGPSTVEERVAQEALETLQLEKRLSLLSHAGRPGSGGDDRSLSSSSSEASHLDVSASSRLTAWPEQSSSSASTSQEGPRPAAAQAAGEAMVGASRPPPKPLRPRQLQEVGRQSSSDSGIATGSHSSYSSSLSSYAGSSLDVWRATDELGSLLSLPAAGAPEPSLCTCLPGTVEYQVPTSLRAHYDTPRSLCLAPRDHSPPSQGSPGNSAARDSGGQTSAGCPSGWLGTRRRGLVMEAPQGSEATLPGPAPGEPWEAGGPHAGPPPAFFSACPVCGGLKVNPPP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Pig

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q18PE1 As Substrate

Q18PE1

Site	PTM Type	Source
S220	Phosphorylation	Uniprot
S246	Phosphorylation	Uniprot
S256	Phosphorylation	Uniprot
Y405	Phosphorylation	Uniprot
S419	Phosphorylation	Uniprot
S425	Phosphorylation	Uniprot

Research Backgrounds

Q18PE1_DOK7_HUMAN

Function:

Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK.

Subcellular Location:

Cell membrane>Peripheral membrane protein. Cell junction>Synapse.
Note: Accumulates at neuromuscular junctions.

Tissue Specificity:

Preferentially expressed in skeletal muscle and heart. Present in thigh muscle, diaphragm and heart but not in the liver or spleen (at protein level).

Subunit Structure:

Homodimer (By similarity). Forms a heterotetramer composed of 2 DOK7 and 2 MUSK molecules which facilitates MUSK trans-autophosphorylation on tyrosine residue and activation (By similarity). Interacts (via IRS-type PTB domain) with MUSK (via cytoplasmic part); requires MUSK phosphorylation.

Family&Domains:

The PH domain mediated binding to phospholipids with phosphoinositol headgroups. Affinity is highest for phosphatidyl 3,4,5-trisphosphate, followed by phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 4,5-bisphosphate (By similarity).

References

1). Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells. Oncotarget, 2017 (PubMed: 29179463)

Application: WB Species: mouse Sample: Sertoli cells

Figure 4:| OLA attenuates mTORC2 complex activity. Sertoli cells cultured on dishes were treated on day 3 with 100-, 200-, 400- or 800 μg/ml OLA for 24 h. Cells treated with vehicle (0.2% DMSO) were used as negative control. Thereafter, cells were washed twice with PBS to remove residual OLA and terminated for immunoblot (IB). Immunoblot analysis to assess the effects of OLA on the expression of mTORC2 complex: Ritor and p-mTOR, downstream proteins: PKC, Akt, p-PKC and p-AktSer473. GAPDH served as protein loading control. Semiquantitative analysis of protein expression in following histogram (mean ± S.E.M., three independent replicates per groups). * p < 0.05; ** p < 0.01.

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DOK7 Antibody - #AF0681