IRF4 Antibody | Affinity Biosciences

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Western blot analysis on HeLa cell lysates using IRF4 Antibody,The lane on the left was treated with the antigen-specific peptide.

Product:	IRF4 Antibody
Catalog:	AF0692
Description:	Rabbit polyclonal antibody to IRF4
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Horse, Dog
Mol.Wt.:	45kDa; 52kD(Calculated).
Uniprot:	Q15306
RRID:	AB_2834257

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Horse(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

IRF4 Antibody detects endogenous levels of total IRF4.

RRID:

AB_2834257
Cite Format: Affinity Biosciences Cat# AF0692, RRID:AB_2834257.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Interferon regulatory factor 4; IRF 4; IRF-4; Irf4; IRF4_HUMAN; LSIRF; Lymphocyte specific interferon regulatory factor; Lymphocyte specific IRF; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; MUM 1; MUM1; NF EM5; NF-EM5; NFEM5; PU.1 interaction partner; Sfpi1/PU.1 interaction partner; Transcriptional activator PIP;

Immunogens

Immunogen:

Uniprot:

Q15306(IRF4_HUMAN) >>Visit HPA database.

Gene(ID):

IRF4(3662)

Expression:

Q15306 IRF4_HUMAN:

Lymphoid cells.

Description:

IRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.

Sequence:

Q15306 IRF4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNLEGGGRGGEFGMSAVSCGNGKLRQWLIDQIDSGKYPGLVWENEEKSIFRIPWKHAGKQDYNREEDAALFKAWALFKGKFREGIDKPDPPTWKTRLRCALNKSNDFEELVERSQLDISDPYKVYRIVPEGAKKGAKQLTLEDPQMSMSHPYTMTTPYPSLPAQQVHNYMMPPLDRSWRDYVPDQPHPEIPYQCPMTFGPRGHHWQGPACENGCQVTGTFYACAPPESQAPGVPTEPSIRSAEALAFSDCRLHICLYYREILVKELTTSSPEGCRISHGHTYDASNLDQVLFPYPEDNGQRKNIEKLLSHLERGVVLWMAPDGLYAKRLCQSRIYWDGPLALCNDRPNKLERDQTCKLFDTQQFLSELQAFAHHGRSLPRFQVTLCFGEEFPDPQRQRKLITAHVEPLLARQLYYFAQQNSGHFLRGYDLPEHISNPEDYHRSIRHSSIQE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

100

Pig

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15306 As Substrate

Q15306

Site	PTM Type	Enzyme	Source
K36	Ubiquitination		Uniprot
Y37	Phosphorylation		Uniprot
S48	Phosphorylation		Uniprot
K59	Ubiquitination		Uniprot
Y62	Phosphorylation		Uniprot
K103	Acetylation		Uniprot
K103	Ubiquitination		Uniprot
Y122	Phosphorylation		Uniprot
K123	Ubiquitination		Uniprot
Y125	Phosphorylation		Uniprot
S177	Phosphorylation		Uniprot
Y181	Phosphorylation		Uniprot
Y192	Phosphorylation		Uniprot
R201	Methylation		Uniprot
K264	Ubiquitination		Uniprot
K306	Ubiquitination		Uniprot
Y325	Phosphorylation		Uniprot
K327	Ubiquitination		Uniprot
K349	Ubiquitination		Uniprot
K357	Ubiquitination		Uniprot
K399	Ubiquitination		Uniprot
Y428	Phosphorylation		Uniprot
Y440	Phosphorylation		Uniprot
S447	Phosphorylation	O75116 (ROCK2)	Uniprot
S448	Phosphorylation	O75116 (ROCK2)	Uniprot

Research Backgrounds

Q15306_IRF4_HUMAN

Function:

Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity).

PTMs:

Phosphorylation by ROCK2 regulates IL-17 and IL-21 production.

Subcellular Location:

Nucleus.

Tissue Specificity:

Lymphoid cells.

Subunit Structure:

Interacts with the BATF-JUNB heterodimer. Interacts with BATF (via bZIP domain); the interaction is direct (By similarity). Interacts with SPIB and DEF6. Directly interacts with NLRP3 in the nucleus of Th2 cells; this interaction enhances IRF4 ability to bind to the IL4 promoter and is required for optimal IRF4-dependent IL4 transcription (By similarity).

Family&Domains:

Belongs to the IRF family.

Research Fields

· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)

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IRF4 Antibody - #AF0692