GK2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Colo205, using GK2 Antibody.

Product:	GK2 Antibody
Catalog:	AF0725
Description:	Rabbit polyclonal antibody to GK2
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Rabbit
Mol.Wt.:	61kDa; 61kD(Calculated).
Uniprot:	Q14410
RRID:	AB_2834255

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Rabbit(82%)

Clonality:

Polyclonal

Specificity:

GK2 Antibody detects endogenous levels of total GK2.

RRID:

AB_2834255
Cite Format: Affinity Biosciences Cat# AF0725, RRID:AB_2834255.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ATP:glycerol 3 phosphotransferase; EC 2.7.1.30; GK 2; GK; GKP2; GKTA; Glycerokinase; Glycerol kinase 2; Glycerol kinase testis specific 2;

Immunogens

Immunogen:

Uniprot:

Q14410(GLPK2_HUMAN) >>Visit HPA database.

Gene(ID):

GK2(2712)

Description:

GK2 Key enzyme in the regulation of glycerol uptake and metabolism. Belongs to the FGGY kinase family.

Sequence:

Q14410 GLPK2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAPKTAAVGPLVGAVVQGTNSTRFLVFNSKTAELLSHHKVELTQEFPKEGWVEQDPKEILQSVYECIARTCEKLDELNIDISNIKAVGVSNQRETTVIWDKLTGEPLYNAVVWLDLRTQTTVEDLSKKIPGNSNFVKSKTGLPLSTYFSAVKLRWMLDNVRNVQKAVEEGRALFGTIDSWLIWSLTGGVNGGVHCTDVTNASRTMLFNIHSLEWDKELCDFFEIPMDLLPNVFSSSEIYGLIKTGALEGVPISGCLGDQCAALVGQMCFQEGQAKNTYGTGCFLLCNTGRKCVFSEHGLLTTVAYKLGREKPAYYALEGSVAIAGAVIRWLRDNLGIIETSGDIERLAKEVGTSYGCYFVPAFSGLYAPYWEPSARGILCGLTQFTNKCHIAFAALEAVCFQTREILEAMNRDCGIPLRHLQVDGGMTNNKVLMQLQADILHIPVIKPFMPETTALGAAMAAGAAEGVSVWSLEPQALSVLRMERFEPQIQATESEIRYATWKKAVMKSMGWVTSQSPEGGDPSIFSSLPLGFFIVSSMVMLIGARYISGVP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Horse

Bovine

Sheep

Dog

Pig

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14410 As Substrate

Q14410

Site	PTM Type	Source
Y109	Phosphorylation	Uniprot
K140	Ubiquitination	Uniprot
Y315	Phosphorylation	Uniprot
Y316	Phosphorylation	Uniprot
Y500	Phosphorylation	Uniprot

Research Backgrounds

Q14410_GLPK2_HUMAN

Function:

Key enzyme in the regulation of glycerol uptake and metabolism.

Subcellular Location:

Mitochondrion outer membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm.
Note: In sperm the majority of the enzyme is bound to mitochondria.

Family&Domains:

Belongs to the FGGY kinase family.

Research Fields

· Metabolism > Lipid metabolism > Glycerolipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

GK2 Antibody - #AF0725