GCKR Antibody - #AF0569

(3)  
Product: GCKR Antibody
Catalog: AF0569
Description: Rabbit polyclonal antibody to GCKR
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Rabbit, Dog
Mol.Wt.: 68kDa; 69kD(Calculated).
Uniprot: Q14397
RRID: AB_2834254

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Rabbit(91%), Dog(82%)
Clonality:
Polyclonal
Specificity:
GCKR Antibody detects endogenous levels of total GCKR.
RRID:
AB_2834254
Cite Format: Affinity Biosciences Cat# AF0569, RRID:AB_2834254.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FGQTL5; GCK; GCKR; GCKR_HUMAN; GKRP; GLRE; glucokinase (hexokinase 4) regulator; Glucokinase; Glucokinase regulator; Glucokinase regulatory protein; Hexokinase 4 regulator; Hexokinase type IV; Hexokinase-4; Hexokinase-D; HK IV; HK4; OTTHUMP00000158533;

Immunogens

Immunogen:
Uniprot:

Q14397(GCKR_HUMAN) >>Visit HPA database.

Gene(ID):
GCKR(2646)
Expression:
Q14397 GCKR_HUMAN:

Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen.

Description:
This gene encodes a protein belonging to the GCKR subfamily of the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. This gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY).
Sequence:
MPGTKRFQHVIETPEPGKWELSGYEAAVPITEKSNPLTQDLDKADAENIVRLLGQCDAEIFQEEGQALSTYQRLYSESILTTMVQVAGKVQEVLKEPDGGLVVLSGGGTSGRMAFLMSVSFNQLMKGLGQKPLYTYLIAGGDRSVVASREGTEDSALHGIEELKKVAAGKKRVIVIGISVGLSAPFVAGQMDCCMNNTAVFLPVLVGFNPVSMARNDPIEDWSSTFRQVAERMQKMQEKQKAFVLNPAIGPEGLSGSSRMKGGSATKILLETLLLAAHKTVDQGIAASQRCLLEILRTFERAHQVTYSQSPKIATLMKSVSTSLEKKGHVYLVGWQTLGIIAIMDGVECIHTFGADFRDVRGFLIGDHSDMFNQKAELTNQGPQFTFSQEDFLTSILPSLTEIDTVVFIFTLDDNLTEVQTIVEQVKEKTNHIQALAHSTVGQTLPIPLKKLFPSIISITWPLLFFEYEGNFIQKFQRELSTKWVLNTVSTGAHVLLGKILQNHMLDLRISNSKLFWRALAMLQRFSGQSKARCIESLLRAIHFPQPLSDDIRAAPISCHVQVAHEKEQVIPIALLSLLFRCSITEAQAHLAAAPSVCEAVRSALAGPGQKRTADPLEILEPDVQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
91
Pig
82
Dog
82
Horse
73
Bovine
70
Sheep
67
Xenopus
55
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14397 As Substrate

Site PTM Type Enzyme
K164 Acetylation
T280 Phosphorylation
T306 Phosphorylation
T315 Phosphorylation
S321 Phosphorylation

Research Backgrounds

Function:

Regulates glucokinase (GCK) by forming an inactive complex with this enzyme. Acts by promoting GCK recruitment to the nucleus, possibly to provide a reserve of GCK that can be quickly released in the cytoplasm after a meal. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity.

Subcellular Location:

Cytoplasm. Nucleus. Mitochondrion.
Note: Under low glucose concentrations, GCKR associates with GCK and the inactive complex is recruited to the hepatocyte nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen.

Subunit Structure:

Interacts (fructose 6-phosphate bound form) with GCK.

Family&Domains:

Fructose 1-phosphate and fructose 6-phosphate compete for the same binding site.

Belongs to the GCKR family.

Restrictive clause

 

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