Product: SHD Antibody
Catalog: DF4496
Description: Rabbit polyclonal antibody to SHD
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Sheep, Dog
Mol.Wt.: 130 KD; 38kD(Calculated).
Uniprot: Q96IW2
RRID: AB_2836847

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(88%), Sheep(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
SHD Antibody detects endogenous levels of total SHD.
RRID:
AB_2836847
Cite Format: Affinity Biosciences Cat# DF4496, RRID:AB_2836847.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

SH2 domain-containing adapter protein D; SHD; SHD_HUMAN; Src homology 2 domain containing transforming protein D;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAKWLRDYLSFGGRRPPPQPPTPDYTESDILRAYRAQKNLDFEDPYEDAESRLEPDPAGPGDSKNPGDAKYGSPKHRLIKVEAADMARAKALLGGPGEELEADTEYLDPFDAQPHPAPPDDGYMEPYDAQWVMSELPGRGVQLYDTPYEEQDPETADGPPSGQKPRQSRMPQEDERPADEYDQPWEWKKDHISRAFAVQFDSPEWERTPGSAKELRRPPPRSPQPAERVDPALPLEKQPWFHGPLNRADAESLLSLCKEGSYLVRLSETNPQDCSLSLRSSQGFLHLKFARTRENQVVLGQHSGPFPSVPELVLHYSSRPLPVQGAEHLALLYPVVTQTP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Sheep
88
Dog
88
Horse
75
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96IW2 As Substrate

Site PTM Type Enzyme
Y46 Phosphorylation
Y144 Phosphorylation
S222 Phosphorylation

Research Backgrounds

Function:

May function as an adapter protein.

PTMs:

Tyrosine phosphorylated by ABL.

References

1). Interleukin 17A deficiency alleviates fluoride-induced testicular injury by inhibiting the immune response and apoptosis. Chemosphere, 2021 (PubMed: 33297146) [IF=8.1]

Application: WB    Species: Mice    Sample: testis

Fig. 5. IL-17A mediates the effect of excessive F on the testosterone level in serum and the expression of testosterone secretion-related proteins in testis (A) The content of testosterone in serum of mice (n ¼ 13. Three times repetition). (B) The expression of testosterone secretion-related proteins in testis (n ¼ 13. Three times repetition). Statistical analysis was carried out using one-way ANOVA with Dunnett’s test. Error bars denote the mean ± s.e.m. *P < 0.05, **P < 0.01.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.