Product: G3BP2 Antibody
Catalog: DF4387
Description: Rabbit polyclonal antibody to G3BP2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 55 KD; 54kD(Calculated).
Uniprot: Q9UN86
RRID: AB_2836742

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
G3BP2 Antibody detects endogenous levels of total G3BP2.
RRID:
AB_2836742
Cite Format: Affinity Biosciences Cat# DF4387, RRID:AB_2836742.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

G3BP 2; G3BP-2; G3BP2; G3BP2_HUMAN; GAP SH3 domain binding protein 2; GAP SH3 domain-binding protein 2; GTPase activating protein (SH3 domain) binding protein 2; Ras GTPase activating protein SH3 domain binding protein 2; Ras GTPase-activating protein-binding protein 2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MVMEKPSPLLVGREFVRQYYTLLNKAPEYLHRFYGRNSSYVHGGVDASGKPQEAVYGQNDIHHKVLSLNFSECHTKIRHVDAHATLSDGVVVQVMGLLSNSGQPERKFMQTFVLAPEGSVPNKFYVHNDMFRYEDEVFGDSEPELDEESEDEVEEEQEERQPSPEPVQENANSGYYEAHPVTNGIEEPLEESSHEPEPEPESETKTEELKPQVEEKNLEELEEKSTTPPPAEPVSLPQEPPKAFSWASVTSKNLPPSGTVSSSGIPPHVKAPVSQPRVEAKPEVQSQPPRVREQRPRERPGFPPRGPRPGRGDMEQNDSDNRRIIRYPDSHQLFVGNLPHDIDENELKEFFMSFGNVVELRINTKGVGGKLPNFGFVVFDDSEPVQRILIAKPIMFRGEVRLNVEEKKTRAARERETRGGGDDRRDIRRNDRGPGGPRGIVGGGMMRDRDGRGPPPRGGMAQKLGSGRGTGQMEGRFTGQRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
71
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UN86 As Substrate

Site PTM Type Enzyme
K5 Ubiquitination
S7 Phosphorylation
K25 Ubiquitination
R32 Methylation
S48 Phosphorylation
K50 Ubiquitination
Y56 Phosphorylation
K64 Ubiquitination
S67 Phosphorylation
K76 Acetylation
K76 Ubiquitination
K107 Ubiquitination
T111 Phosphorylation
K123 Ubiquitination
S141 Phosphorylation
S149 Phosphorylation
S163 Phosphorylation
Y175 Phosphorylation
Y176 Phosphorylation
T206 Phosphorylation
S225 Phosphorylation
T226 Phosphorylation
T227 Phosphorylation
S235 Phosphorylation
K281 Sumoylation
S353 Phosphorylation
T364 Phosphorylation
K370 Acetylation
K370 Ubiquitination
K392 Ubiquitination
R432 Methylation
R438 Methylation
R447 Methylation
R452 Methylation
R457 Methylation
S466 Phosphorylation
R468 Methylation
R476 Methylation

Research Backgrounds

Function:

Scaffold protein that plays an essential role in cytoplasmic stress granule formation which acts as a platform for antiviral signaling.

PTMs:

Arg-457 and Arg-468 are dimethylated, probably to asymmetric dimethylarginine.

(Microbial infection) Cleaved by foot-and-mouth disease virus leader protease; this cleavage suppresses the formation of cytoplasmic stress granules.

Subcellular Location:

Cytoplasm. Cytoplasm>Stress granule.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Forms homooligomers. Forms heterodimers with G3BP1. Interacts with NFKBIA (via N-terminus). Interacts with USP10. Interacts with PABPC1.

(Microbial infection) Interacts with Sindbis virus non-structural protein 3 (via C-terminus); this interaction inhibits the formation of host stress granules on viral mRNAs and the nsp3-G3BP2 complexes bind viral RNAs and probably orchestrate the assembly of viral replication complexes.

References

1). Astragaloside IV derivative HHQ16 ameliorates infarction-induced hypertrophy and heart failure through degradation of lncRNA4012/9456. Signal transduction and targeted therapy, 2023 (PubMed: 37857609) [IF=40.8]

Application: WB    Species: Mouse    Sample:

Fig. 5 Lnc9456 interacts with G3BP2 and promotes the nuclear translocation of p65 subunit of NF-κB. a The top 7 proteins that may bind to lnc9456 were predicted by catRAPID omics module. b The top 20 of Gene Ontology (GO) analysis of interacting proteins pulled down by biotinylated lnc9456 probe and identified by mass spectrometry. c Representative western blotting (upper) and its quantification (lower) of G3BP2 in myocardial tissues derived from mice at the indicated time post-LADL (n = 4). d Representative western blotting (upper) and its quantification (lower) of G3BP2 in HL-1 mouse cardiomyocytes transfected with smart silencer RNA of lnc9456 (ssRNA-lnc9456) or its negative control (ssRNA-NC) for 72 h (n = 4). e Representative western blotting (left) and its quantification (right) of G3BP2 in HL-1 mouse cardiomyocytes transfected with control plasmid (OE-NC) or lnc9456 overexpression plasmid (OE-lnc9456) for 48 h (n = 4). f RNA pull-down and western blotting detection for the binding of G3BP2 to lnc9456 in HL-1 mouse cardiomyocytes treated as mentioned in e. g Co-IP detection for the binding of G3BP2 to IκBα in HL-1 mouse cardiomyocytes treated as mentioned in e. h Immunofluorescence staining for cellular localization of NF-κB p65 (green) in HL-1 mouse cardiomyocytes treated as mentioned in e (n = 3). Data are presented as the means ± SEM. *P 

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