Product: TFAM Antibody
Catalog: DF3232
Description: Rabbit polyclonal antibody to TFAM
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 0 KD; 29kD(Calculated).
Uniprot: Q00059
RRID: AB_2835612

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
TFAM Antibody detects endogenous levels of total TFAM.
RRID:
AB_2835612
Cite Format: Affinity Biosciences Cat# DF3232, RRID:AB_2835612.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

anscription factor 6-like 1; Mitochondrial transcription factor 1; mitochondrial transcription factor A; MtTF1; mtTFA; TCF 6; TCF-6; TCF6; TCF6L1; TCF6L2; TCF6L3; TFAM; TFAM_HUMAN; Transcription factor 6; Transcription factor 6 like 2 (mitochondrial transcription factor); Transcription factor 6 like 2; Transcription factor 6-like 2; transcription factor 6-like 3; Transcription factor A, mitochondrial; Transcription factor A, mitochondrial; Transcription factor A, mitochondrial precursor;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAFLRSMWGVLSALGRSGAELCTGCGSRLRSPFSFVYLPRWFSSVLASCPKKPVSSYLRFSKEQLPIFKAQNPDAKTTELIRRIAQRWRELPDSKKKIYQDAYRAEWQVYKEEISRFKEQLTPSQIMSLEKEIMDKHLKRKAMTKKKELTLLGKPKRPRSAYNVYVAERFQEAKGDSPQEKLKTVKENWKNLSDSEKELYIQHAKEDETRYHNEMKSWEEQMIEVGRKDLLRRTIKKQRKYGAEEC

PTMs - Q00059 As Substrate

Site PTM Type Enzyme
K52 Ubiquitination
S55 Phosphorylation P17612 (PRKACA)
S56 Phosphorylation P17612 (PRKACA)
S61 Phosphorylation P17612 (PRKACA)
K62 Ubiquitination
K69 Methylation
K76 Ubiquitination
R82 Methylation
Y99 Phosphorylation
K111 Acetylation
K111 Ubiquitination
K118 Ubiquitination
T122 Phosphorylation P24941 (CDK2)
S124 Phosphorylation P24941 (CDK2)
S128 Phosphorylation
K146 Methylation
K147 Methylation
S160 Phosphorylation P17612 (PRKACA)
Y165 Phosphorylation
S177 Phosphorylation
K186 Ubiquitination
K190 Ubiquitination
S193 Phosphorylation
S195 Phosphorylation
K197 Ubiquitination
Y200 Phosphorylation
C246 S-Nitrosylation

Research Backgrounds

Function:

Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA.

PTMs:

Phosphorylation by PKA within the HMG box 1 impairs DNA binding and promotes degradation by the AAA+ Lon protease.

Subcellular Location:

Mitochondrion. Mitochondrion matrix>Mitochondrion nucleoid.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Monomer; binds DNA as a monomer. Homodimer. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT. In this complex TFAM recruits POLRMT to the promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Upon metabolic stress, forms a complex composed of FOXO3, SIRT3, TFAM and POLRMT. Interacts with TFB1M and TFB2M. Interacts with CLPX; this enhances DNA-binding.

Family&Domains:

Binds DNA via its HMG boxes. When bound to the mitochondrial light strand promoter, bends DNA into a U-turn shape, each HMG box bending the DNA by 90 degrees.

Research Fields

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

References

1). Ginsenoside Rd promotes omentin secretion in adipose through TBK1-AMPK to improve mitochondrial biogenesis via WNT5A/Ca2+ pathways in heart failure. Redox biology, 2023 (PubMed: 36652744) [IF=10.7]

Application: WB    Species: Mouse    Sample:

Fig. 5 Adipose tissue-specific omentin overexpression inhibited WNT5A/Ca2+ signaling pathway and improved mitochondrial biogenesis to ameliorate myocardial injury in HF mice. (A) Serum omentin level of HF patients and healthy subjects. A total of 58 HF patients and 38 healthy subjects were enrolled. (B) The omentin serum content of adipose tissue-specific omentin overexpression by intravenous injection of AAV-omentin and negative control by intravenous injection of AAV-NC (n = 8). (C) The expression of omentin in the inguinal fat of mice with adipose tissue-specific omentin overexpression was detected by immunohistochemistry (n = 4). Scale bar = 200 μm. (D) Representative echocardiographs of mice with AAV-omentin overexpression and the statistical results of (E) LV EF, (F) LV FS, (G) stroke volume, (H) LVPW; d, (I) IVS; d, (J) RWT and (K) LV Mass were presented (n = 8). (L) Representative TTC staining images of heart tissues of mice with AAV-omentin overexpression (n = 6). (M) Representative images of H&E and Masson staining of heart tissues of mice with AAV-omentin overexpression (n = 3), scale bar = 250 μm. (N) The serum content of BNP in mice with AAV-omentin overexpression (n = 8). (O) The serum CK activity in mice with AVV-omentin overexpression (n = 8). (P) The protein expression level of WNT5A, Frizzled2 and p-CAMKII/CAMKII were determined by Western blot. β-actin was used as a loading control (n = 5). (Q) The protein expression level of PGC-1α, NRF1, NRF2 and TFAM were determined by Western blot in mice with AAV-omentin overexpression. β-actin was used as a loading control (n = 5). Values are expressed as the means ± SD. (A) Unpaired Student's two-tailed t-test; (B–Q) One-way ANOVA followed by the Dunnett's post hoc test. #P < 0.05, ##P < 0.01, ###P < 0.001 vs. Sham; *P < 0.05, **P < 0.01, ***P < 0.001 vs. CAL.

2). Exposure to Bisphenol A Caused Hepatoxicity and Intestinal Flora Disorder in Rats. International Journal of Molecular Sciences, 2022 (PubMed: 35887390) [IF=5.6]

Application: WB    Species: Rat    Sample: liver tissues

Figure 3. Effects of BPA on the liver SIRT1/PGC-1α pathway. (A) The relative protein levels of PGC-1α, Nrf2, and SIRT1. (B–D) Values of quantitative analysis (n = 4). (E) Relative mRNA levels of SIRT1, PGC-1α, Nrf1, Nrf2, TNF-α, and IL-1β. (F) Percentage of immunostaining for Sirt1. (G) Immunohistochemistry shows the expression of hepatic SIRT1 (400× magnifications). (H) The relative protein levels of TFAM and (I) the relative protein levels of TNF-α. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.01 vs. control group. ns: no significance.

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