PDHA1 Mouse Monoclonal Antibody | Affinity Biosciences

Product:	PDHA1 Mouse Monoclonal Antibody
Catalog:	BF8861
Description:	Mouse monoclonal antibody to PDHA1
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Rat, Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.:	43kDa; 43kD(Calculated).
Uniprot:	P08559

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8861]

Specificity:

PDHA1 Mouse Monoclonal Antibody detects endogenous levels of total PDHA1.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ODPA_HUMAN; PDH; PDHA; PDHA1; PDHCE1A; PDHE1 A type I; PDHE1-A type I; PHE1A; Pyruvate Dehydrogenase (lipoamide) alpha 1; Pyruvate dehydrogenase complex, E1 alpha polypeptide 1; Pyruvate Dehydrogenase E1 alpha; Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial;

Immunogens

Immunogen:

Uniprot:

P08559(ODPA_HUMAN) >>Visit HPA database.

Gene(ID):

PDHA1(5160)

Expression:

P08559 ODPA_HUMAN:

Ubiquitous.

Description:

The pyruvate dehydrogenase complex catalyzes the conversion of pyruvate and CoA into acetyl-CoA and CO2 in the presence of NAD+. Acetyl-CoA then goes into the citric acid cycle where it reacts with oxaloacetate to form citrate. Acetyl-CoA is also used for fatty acid and cholesterol biosynthesis. The reaction of oxidative decarboxylation of pyruvate therefore serves as a critical link between glycolysis and the citric acid cycle and lipid metabolism. In mammalian cells, the pyruvate dehydrogenase complex is located in the mitochondrial matrix (1). This complex is comprised of three enzymes: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3). Pyruvate dehydrogenase (E1) consists of two subunits: α and β. This enzyme catalyzes the removal of CO2 from pyruvate. Mutations in the α subunits of pyruvate dehydrogenase (E1) lead to congenital defects that are usually associated with lactic acidosis, neurodegeneration and early death (2).

Sequence:

P08559 ODPA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRKMLAAVSRVLSGASQKPASRVLVASRNFANDATFEIKKCDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS

PTMs - P08559 As Substrate

P08559

Site	PTM Type	Enzyme	Source
K18	Acetylation		Uniprot
K18	Ubiquitination		Uniprot
K39	Acetylation		Uniprot
K39	Ubiquitination		Uniprot
K63	Acetylation		Uniprot
K63	Ubiquitination		Uniprot
K77	Acetylation		Uniprot
K83	Acetylation		Uniprot
K83	Ubiquitination		Uniprot
T116	Phosphorylation		Uniprot
Y118	Phosphorylation		Uniprot
T139	Phosphorylation		Uniprot
S152	Phosphorylation		Uniprot
Y227	Phosphorylation		Uniprot
T231	Phosphorylation		Uniprot
S232	Phosphorylation	Q15118 (PDK1) , O15530 (PDPK1) , Q15119 (PDK2)	Uniprot
T240	Phosphorylation		Uniprot
Y242	Phosphorylation		Uniprot
Y243	Phosphorylation		Uniprot
K244	Acetylation		Uniprot
K244	Ubiquitination		Uniprot
R245	Methylation		Uniprot
Y272	Phosphorylation		Uniprot
K277	Acetylation		Uniprot
Y289	Phosphorylation		Uniprot
S293	Phosphorylation	Q16654 (PDK4) , Q15119 (PDK2) , O15530 (PDPK1) , Q15120 (PDK3) , Q15118 (PDK1)	Uniprot
S295	Phosphorylation		Uniprot
S300	Phosphorylation	Q15119 (PDK2) , Q15118 (PDK1) , Q16654 (PDK4) , Q15120 (PDK3) , O15530 (PDPK1)	Uniprot
Y301	Phosphorylation		Uniprot
T303	Phosphorylation		Uniprot
K313	Acetylation		Uniprot
K321	Acetylation		Uniprot
K321	Ubiquitination		Uniprot
K336	Acetylation		Uniprot
K344	Ubiquitination		Uniprot
Y366	Phosphorylation		Uniprot
Y369	Phosphorylation		Uniprot
K385	Methylation		Uniprot

Research Backgrounds

P08559_ODPA_HUMAN

Function:

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

PTMs:

Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme; for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation.

Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3 (By similarity).

Subcellular Location:

Mitochondrion matrix.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Heterotetramer of two PDHA1 and two PDHB subunits. The heterotetramer interacts with DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules.

Research Fields

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).

· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

AFfirm™ PDHA1 Mouse Monoclonal Antibody - #BF8861