ANGPT4 Mouse Monoclonal Antibody | Affinity Biosciences

Product:	ANGPT4 Mouse Monoclonal Antibody
Catalog:	BF8642
Description:	Mouse monoclonal antibody to ANGPT4
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	57 kDa; 57kD(Calculated).
Uniprot:	Q9Y264

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$140 ~~280~~	In stock

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8642]

Specificity:

ANGPT4 Mouse Monoclonal Antibody detects endogenous levels of total ANGPT4.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AGP4; ANG-3; ANG-4; ANG3; ANG4; Angiopoietin 3; Angiopoietin 4; Angiopoietin-3; Angiopoietin-4; ANGP4_HUMAN; ANGPT4; dJ824F16.2 (angiopoietin 4); MGC138181; MGC138183; OTTHUMP00000029947;

Immunogens

Immunogen:

Uniprot:

Q9Y264(ANGP4_HUMAN) >>Visit HPA database.

Gene(ID):

ANGPT4(51378)

Expression:

Q9Y264 ANGP4_HUMAN:

Highly expressed in the lung with much lower levels found in other tissues.

Sequence:

Q9Y264 ANGP4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLSQLAMLQGSLLLVVATMSVAQQTRQEADRGCETLVVQHGHCSYTFLLPKSEPCPPGPEVSRDSNTLQRESLANPLHLGKLPTQQVKQLEQALQNNTQWLKKLERAIKTILRSKLEQVQQQMAQNQTAPMLELGTSLLNQTTAQIRKLTDMEAQLLNQTSRMDAQMPETFLSTNKLENQLLLQRQKLQQLQGQNSALEKRLQALETKQQEELASILSKKAKLLNTLSRQSAALTNIERGLRGVRHNSSLLQDQQHSLRQLLVLLRHLVQERANASAPAFIMAGEQVFQDCAEIQRSGASASGVYTIQVSNATKPRKVFCDLQSSGGRWTLIQRRENGTVNFQRNWKDYKQGFGDPAGEHWLGNEVVHQLTRRAAYSLRVELQDWEGHEAYAQYEHFHLGSENQLYRLSVVGYSGSAGRQSSLVLQNTSFSTLDSDNDHCLCKCAQVMSGGWWFDACGLSNLNGVYYHAPDNKYKMDGIRWHYFKGPSYSLRASRMMIRPLDI

PTMs - Q9Y264 As Substrate

Q9Y264

Site	PTM Type	Source
S72	Phosphorylation	Uniprot
T150	Phosphorylation	Uniprot
T226	Phosphorylation	Uniprot
S228	Phosphorylation	Uniprot

Research Backgrounds

Q9Y264_ANGP4_HUMAN

Function:

Binds to TEK/TIE2, modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2. Promotes endothelial cell survival, migration and angiogenesis.

Subcellular Location:

Secreted.

Tissue Specificity:

Highly expressed in the lung with much lower levels found in other tissues.

Subunit Structure:

Homodimer; disulfide-linked. Interacts with TEK/TIE2.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

AFfirm™ ANGPT4 Mouse Monoclonal Antibody - #BF8642