AFfirm™ RPS6 Mouse Monoclonal Antibody - #BF8412
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Fold/Unfold
40S ribosomal protein S6; Air8; NP33; Phosphoprotein NP33; Pp30; Ribosomal protein S6; RP S6; rps6; RS6; RS6_HUMAN; S6; S6 Ribosomal Protein;
Immunogens
A synthesized peptide derived from human RPS6.
- P62753 RS6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK
PTMs - P62753 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K2 | Methylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| C12 | S-Nitrosylation | Uniprot | |
| K14 | Acetylation | Uniprot | |
| K14 | Ubiquitination | Uniprot | |
| R22 | Methylation | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| Y28 | Phosphorylation | Uniprot | |
| K30 | Acetylation | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| T69 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| K79 | Acetylation | Uniprot | |
| K79 | Ubiquitination | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| T128 | Phosphorylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| R154 | Methylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| K203 | Ubiquitination | Uniprot | |
| Y209 | Phosphorylation | Uniprot | |
| K211 | Acetylation | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| K218 | Acetylation | Uniprot | |
| S235 | Phosphorylation | Q13177 (PAK2) , P51812 (RPS6KA3) , P53355 (DAPK1) , Q05655 (PRKCD) , P23443 (RPS6KB1) , Q96RG2 (PASK) , Q15418 (RPS6KA1) | Uniprot |
| S236 | Phosphorylation | Q05655 (PRKCD) , P23443 (RPS6KB1) , P51812 (RPS6KA3) , Q13177 (PAK2) , Q15418 (RPS6KA1) , Q96RG2 (PASK) | Uniprot |
| S240 | Phosphorylation | Q13177 (PAK2) , P23443 (RPS6KB1) , Q15418 (RPS6KA1) | Uniprot |
| T241 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Q13177 (PAK2) | Uniprot |
| S244 | Phosphorylation | Q15418 (RPS6KA1) , P23443 (RPS6KB1) | Uniprot |
| S246 | Phosphorylation | Uniprot | |
| S247 | Phosphorylation | P23443 (RPS6KB1) | Uniprot |
Research Backgrounds
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.
Specifically hydroxylated (with R stereochemistry) at C-3 of Arg-137 by KDM8.
Belongs to the eukaryotic ribosomal protein eS6 family.
Research Fields
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Genetic Information Processing > Translation > Ribosome.
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
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