AFfirm™ Tyrosine Hydroxylase Mouse Monoclonal Antibody - #BF8114
(2)
Product Info
Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Clonality:
Monoclonal [AFfirm8114]
Specificity:
Tyrosine Hydroxylase Antibody detects endogenous levels of total Tyrosine Hydroxylase.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Dystonia 14; DYT14; DYT5b; EC 1.14.16.2; OTTHUMP00000011225; OTTHUMP00000011226; ple; Protein Pale; TH; The; TY3H_HUMAN; TYH; Tyrosine 3 hydroxylase; Tyrosine 3 monooxygenase; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase; Tyrosine hydroxylase;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Description:
Tyrosine hydroxylase (EC 1.14.16.2) is involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.
Sequence:
- P07101 TY3H_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPTPDATTPQAKGFRRAVSELDAKQAEAIMVRGQGAPGPSLTGSPWPGTAAPAASYTPTPRSPRFIGRRQSLIEDARKEREAAVAAAAAAVPSEPGDPLEAVAFEEKEGKAVLNLLFSPRATKPSALSRAVKVFETFEAKIHHLETRPAQRPRAGGPHLEYFVRLEVRRGDLAALLSGVRQVSEDVRSPAGPKVPWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEIAFQYRHGDPIPRVEYTAEEIATWKEVYTTLKGLYATHACGEHLEAFALLERFSGYREDNIPQLEDVSRFLKERTGFQLRPVAGLLSARDFLASLAFRVFQCTQYIRHASSPMHSPEPDCCHELLGHVPMLADRTFAQFSQDIGLASLGASDEEIEKLSTLYWFTVEFGLCKQNGEVKAYGAGLLSSYGELLHCLSEEPEIRAFDPEAAAVQPYQDQTYQSVYFVSESFSDAKDKLRSYASRIQRPFSVKFDPYTLAIDVLDSPQAVRRSLEGVQDELDTLAHALSAIG
PTMs - P07101 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T3 | Phosphorylation | Uniprot | |
| S19 | Phosphorylation | Q8IW41 (MAPKAPK5) , P49137 (MAPKAPK2) , Q9UQM7 (CAMK2A) | Uniprot |
| S40 | Phosphorylation | P49137 (MAPKAPK2) , P51812 (RPS6KA3) , O75582 (RPS6KA5) | Uniprot |
| S62 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| S71 | Phosphorylation | P49137 (MAPKAPK2) , P51812 (RPS6KA3) , P17612 (PRKACA) , Q9UQM7 (CAMK2A) | Uniprot |
| S118 | Phosphorylation | Uniprot | |
| Y230 | Phosphorylation | Uniprot | |
| Y419 | Phosphorylation | Uniprot | |
| S502 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Plays an important role in the physiology of adrenergic neurons.
Tissue Specificity:
Mainly expressed in the brain and adrenal glands.
Family&Domains:
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Restrictive clause
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For Research Use Only.
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