Product Info

Source:
Mouse
Application:
WB 1:500-1:2000, IHC 1:100-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Monoclonal [AFfirm08(AFB17785)]
Specificity:
Histone H2B mouse monoclonal antibody detects endogenous levels of total Histone H2B.
RRID:
AB_2846198
Cite Format: Affinity Biosciences Cat# BF0722, RRID:AB_2846198.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

dJ221C16.8; H2B histone family, member A; H2B/a; H2B/g; H2B/h; H2B/k; H2B/l; H2B1C_HUMAN; H2BFA; HIST1H2BI; Histone 1, H2bg; Histone cluster 1 H2B family member g; Histone cluster 1, H2bg; Histone H2B type 1-C/E/F/G/I; Histone H2B.1 A; Histone H2B.a; Histone H2B.g; Histone H2B.h; Histone H2B.k; Histone H2B.l; H2B histone family member R; H2B/r; H2B1J_HUMAN; H2BFR; HIST1H2BJ; Histone 1 H2bj; Histone cluster 1 H2bj; Histone H2B type 1-J; Histone H2B.1; Histone H2B.r; GL105; H2B; H2B histone family member Q; H2B-GL105; H2B.1; H2B/q; H2B2E_HUMAN; H2BFQ; H2BGL105; H2BQ; HIST2H2BE; Histone 2 H2be; histone cluster 2, H2be; histone H2B GL105; histone H2B type 2 E; Histone H2B type 2-E; histone H2B type 2E; Histone H2B-GL105; Histone H2B.q; histone H2BGL105; H2B K; H2B type 12; H2B/b; H2B/c; H2B/d; H2B/e; H2B/f; H2B/j; H2B/n; H2B/q; H2B/r; H2B/s; HIRA-interacting protein 1; HIRA-interacting protein 2; Histone H2B type 1-B; Histone H2B type 1-C/E/F/G/I; Histone H2B type 1-D; Histone H2B type 1-H; Histone H2B type 1-J; Histone H2B type 1-K; Histone H2B type 1-L; Histone H2B type 1-M; Histone H2B type 1-N; Histone H2B type 1-O; Histone H2B type 2-E; Histone H2B type 2-F; Histone H2B type 3-B; Histone H2B type F-S; Histone H2B-GL105; Histone H2B.1 A; Histone H2B.1; Histone H2B.1 B; Histone H2B.2; Histone H2B.a; Histone H2B.b; Histone H2B.c; Histone H2B.d; Histone H2B.e; Histone H2B.f; Histone H2B.g; Histone H2B.h; Histone H2B.j; Histone H2B.k; Histone H2B.l; Histone H2B.n; Histone H2B.r; Histone H2B.s;

Immunogens

Immunogen:

Purified recombinant fragment of human Histone H2B expressed in E. Coli.

Uniprot:
Gene(ID):
Sequence:
MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK

PTMs - P06899 As Substrate

Site PTM Type Enzyme
Acetylation
K6 Acetylation
K6 Methylation
K6 Sumoylation
K6 Ubiquitination
S7 Phosphorylation
K12 Acetylation
K13 Acetylation
S15 Phosphorylation
K16 Acetylation
K17 Acetylation
K21 Acetylation
K21 Sumoylation
K21 Ubiquitination
K24 Acetylation
K35 Ubiquitination
S37 Phosphorylation
Y38 Phosphorylation
S39 Phosphorylation
Y41 Phosphorylation
Y43 Phosphorylation
K44 Acetylation
K44 Ubiquitination
K47 Acetylation
K47 Methylation
K47 Sumoylation
K47 Ubiquitination
T53 Phosphorylation
S56 Phosphorylation
S57 Phosphorylation
K58 Methylation
K58 Ubiquitination
S65 Phosphorylation
R73 Methylation
S79 Phosphorylation
R80 Methylation
Y84 Phosphorylation
K86 Ubiquitination
R87 Methylation
S88 Phosphorylation
T89 Phosphorylation
S92 Phosphorylation
R93 Methylation
T97 Phosphorylation
R100 Methylation
K109 Acetylation
K109 Methylation
K109 Sumoylation
K109 Ubiquitination
S113 Phosphorylation
T116 Phosphorylation
K117 Acetylation
K117 Sumoylation
K117 Ubiquitination
T120 Phosphorylation
K121 Acetylation
K121 Sumoylation
K121 Ubiquitination
T123 Phosphorylation
S124 Phosphorylation
K126 Ubiquitination

Research Backgrounds

Function:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.

PTMs:

Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons.

Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription (By similarity). Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.

GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes (By similarity).

Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.

Subcellular Location:

Nucleus. Chromosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Heterodimer H2BC11 and H2AZ1 interacts with VPS72 (via N-terminal domain).

Family&Domains:

Belongs to the histone H2B family.

Research Fields

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

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