Cytochrome C antibody - #BF0714

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Product: Cytochrome C antibody
Catalog: BF0714
Description: Mouse monoclonal antibody to Cytochrome C
Application: WB IHC
Reactivity: Human, Mouse, Rat, Rabbit
Mol.Wt.: 11kDa; 12kD(Calculated).
Uniprot: P99999
RRID: AB_2846192

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB(1:500-1:2000), IHC(1:100-1:1000)
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Rabbit
Clonality:
Monoclonal [AFB17778]
Specificity:
Cytochrome C antibody detects endogenous levels of total Cytochrome C.
RRID:
AB_2846192
Cite Format: Affinity Biosciences Cat# BF0714, RRID:AB_2846192.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CYC; CYC_HUMAN; CYCS; Cytochrome c; Cytochrome c somatic; HCS; THC4;

Immunogens

Immunogen:

Purified recombinant fragment of human Cytochrome C expressed in E. Coli.

Uniprot:

P99999(CYC_HUMAN) >>Visit HPA database.

Gene(ID):
CYCS(54205)
Sequence:
MGDVEKGKKIFIMKCSQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGYSYTAANKNKGIIWGEDTLMEYLENPKKYIPGTKMIFVGIKKKEERADLIAYLKKATNE

PTMs - P99999 As Substrate

Site PTM Type Enzyme
G2 Acetylation
K9 Acetylation
K28 Sumoylation
K28 Ubiquitination
T29 Phosphorylation
K40 Ubiquitination
T41 Phosphorylation
Y47 Phosphorylation
S48 Phosphorylation
Y49 Phosphorylation
T50 Phosphorylation
K54 Ubiquitination
Y68 Phosphorylation
K73 Acetylation
K73 Ubiquitination
K74 Acetylation
K74 Ubiquitination
Y75 Phosphorylation
T79 Phosphorylation
K80 Ubiquitination
K87 Acetylation
K87 Ubiquitination
K88 Acetylation
K89 Acetylation
Y98 Phosphorylation
K100 Acetylation
K100 Methylation
K100 Ubiquitination
K101 Methylation

Research Backgrounds

Function:

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.

Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

PTMs:

Binds 1 heme group per subunit.

Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.

Subcellular Location:

Mitochondrion intermembrane space.
Note: Loosely associated with the inner membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the cytochrome c family.

Research Fields

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

· Metabolism > Energy metabolism > Sulfur metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Melatonin derivatives combat with inflammation-related cancer by targeting the Main Culprit STAT3. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (PubMed: 33248852) [IF=6.7]

Application: WB    Species: human    Sample: HCT-116 cells

Figure 10. (A) Compound P-3 triggered the explosion of ROS in HCT-116 cells. (magnification 20 ×, scale bar = 100 μm) (B) P-3 regulated the expression of proteins involved in Cyto-c/Caspase3 pathway. Data are presented as mean ± SD from three independent experiments. * P < 0.05, ** P<0.01, *** P<0.001 versus the control group.
2). Design, synthesis and biological evaluation of N-anthraniloyl tryptamine derivatives as pleiotropic molecules for the therapy of malignant glioma. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (PubMed: 34091208) [IF=6.7]

Application: WB    Species: Human    Sample: U251 and U87MG cells.

Fig. 7. NP16 induces U87MG and U251 apoptosis and ROS generation at various concentrations (1, 2 and 4 mM). (A, B): images of apoptosis-related key protein (Bax, Bcl-2 and cytochrome c) expression levels of detected by western blotting method in U251 and U87MG cells. b-actin was used as an internal loading control. (CeF) Quantification of Bcl-2/Bax ratio and cytochrome c using ImageJ software. (G) Representative DCFH-DA fluorescence images of U251 and U87MG cells treated by NP16 at 1, 2 and 4 mM (magnification,  10, scale bar ¼ 500 mm). (HeI) Reactive ROS generation ratio qualified by DCFH-DA fluorescence density in U251 and U87MG using image J. All data are expressed as the mean ± SD pooled from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 when compared with the DMSO groups treated by equivalent amounts of DMSO.

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