CD59 Antibody - #BF0017

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Product: CD59 Antibody
Catalog: BF0017
Description: Mouse monoclonal antibody to CD59
Application: WB IHC IF/ICC ELISA FACS
Reactivity: Human
Mol.Wt.: 14.2kDa; 14kD(Calculated).
Uniprot: P13987
RRID: AB_2834066

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000, IF/ICC 1:200-1:1000, FCM 1:200-1:400
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFB2044]
Specificity:
CD59 antibody detects endogenous levels of total CD59.
RRID:
AB_2834066
Cite Format: Affinity Biosciences Cat# BF0017, RRID:AB_2834066.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

16.3A5; 1F5; 1F5 antigen; 20 kDa homologous restriction factor; CD 59; CD_antigen=CD59; CD59; CD59 antigen; CD59 antigen complement regulatory protein; CD59 antigen p18 20; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344); CD59 glycoprotein; CD59 molecule; CD59 molecule complement regulatory protein; CD59_HUMAN; Cd59a; Complement regulatory protein; EJ16; EJ30; EL32; FLJ38134; FLJ92039; G344; HRF 20; HRF-20; HRF20; Human leukocyte antigen MIC11; Ly 6 like protein; Lymphocytic antigen CD59/MEM43; MAC inhibitory protein; MAC IP; MAC-inhibitory protein; MAC-IP; MACIF; MACIP; MEM43; MEM43 antigen; Membrane attack complex (MAC) inhibition factor; Membrane attack complex inhibition factor; Membrane inhibitor of reactive lysis; MGC2354; MIC11; MIN1; MIN2; MIN3; MIRL; MSK21; p18 20; Protectin; Surface antigen recognized by monoclonal antibody 16.3A5; T cell activating protein;

Immunogens

Immunogen:

Purified recombinant fragment of human CD59 expressed in E. Coli.

Uniprot:

P13987(CD59_HUMAN) >>Visit HPA database.

Gene(ID):
CD59(966)
Description:
This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation.
Sequence:
MGIQGGSVLFGLLLVLAVFCHSGHSLQCYNCPNPTADCKTAVNCSSDFDACLITKAGLQVYNKCWKFEHCNFNDVTTRLRENELTYYCCKKDLCNFNEQLENGGTSLSEKTVLLLVTPFLAAAWSLHP

PTMs - P13987 As Substrate

Site PTM Type Enzyme
Y29 Phosphorylation
N43 N-Glycosylation
K63 Acetylation
K66 Acetylation
T76 O-Glycosylation
T77 O-Glycosylation
Y86 Phosphorylation
Y87 Phosphorylation
K90 Ubiquitination
K91 Ubiquitination

Research Backgrounds

Function:

Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.

PTMs:

N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine. Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.

Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes.

Subcellular Location:

Cell membrane>Lipid-anchor. Secreted.
Note: Soluble form found in a number of tissues.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with T-cell surface antigen CD2.

Research Fields

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

References

1). The Histone Deacetylase Inhibitor I1 Induces Differentiation of Acute Leukemia Cells With MLL Gene Rearrangements via Epigenetic Modification. Frontiers in Pharmacology (PubMed: 35571127) [IF=5.6]

Application: WB    Species: Human    Sample: MOLM-13 and THP-1 cells

FIGURE 7 The RT-PCR and Western blotting analysis of cell differentiation related genes in MOLM-13 and THP-1 cell lines. (A) The effects of I1 on the mRNA expression of CD59, CD13, HLA-DRA and CIITA evaluated by RT-PCR. MOLM-13 and THP-1 cells were incubated with 1, or 0.7 μM I1 repectively, for 24, 48, or 72 h (B) H3, Ac-H3, H4, Ac-H4, CD59, CD13, HLA-DRA and CIITA protein levels measured by Western blotting analysis. (C) Graph bars show the protein expression visualized and quantified by AI600 imager. MOLM-13 were incubated with 0.25, 0.5, 1 μM I1, or 0.5 μM SAHA for 72 h. THP-1 cells were incubated with 0.175, 0.35, 0.7 μM I1, or 0.7 μM SAHA for 72 h(*p < 0.05, **p < 0.01).

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