NEFL Antibody - #BF0117

(2)  
Product: NEFL Antibody
Catalog: BF0117
Description: Mouse monoclonal antibody to NEFL
Application: WB IHC IF/ICC ELISA FACS
Reactivity: Human
Mol.Wt.: 62kDa; 62kD(Calculated).
Uniprot: P07196
RRID: AB_2833978

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000, IF/ICC 1:200-1:1000, FCM 1:200-1:400
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFB1955]
Specificity:
NEFL antibody detects endogenous levels of total NEFL.
RRID:
AB_2833978
Cite Format: Affinity Biosciences Cat# BF0117, RRID:AB_2833978.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

150kDa medium; 160 kDa neurofilament protein; 2 kDa neurofilament protein; 200 kDa neurofilament protein; 68 kDa neurofilament protein; AI847934; CMT1F; CMT2CC; CMT2E; FLJ53642; KIAA0845; light molecular weight neurofilament protein; Micro glutamic acid-rich protein; mKIAA0845; Nef3; NEFH; NEFL; NEFM; Neurofilament 3; Neurofilament heavy polypeptide 200kDa; Neurofilament heavy polypeptide; Neurofilament light polypeptide 68kDa; Neurofilament light polypeptide; Neurofilament medium polypeptide 150kDa; Neurofilament medium polypeptide; Neurofilament protein heavy polypeptide; Neurofilament protein light chain; Neurofilament protein light polypeptide; Neurofilament protein medium polypeptide; neurofilament subunit NF-L; Neurofilament triplet H protein; Neurofilament triplet L protein; Neurofilament triplet M protein; neurofilament-3 (150 kD medium); NF-H; NF-L; NF-M; NF160; NF165; NF200; NF68; NFH; NFL; NFM; NFM_HUMAN; PPP1R110; protein phosphatase 1, regulatory subunit 110;

Immunogens

Immunogen:

Purified recombinant fragment of human NEFL expressed in E. Coli.

Uniprot:

P07196(NFL_HUMAN) >>Visit HPA database.

Gene(ID):
NEFL(4747)
Description:
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.
Sequence:
MSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKDKEEAEEEEAAEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKD

PTMs - P07196 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S3 Phosphorylation
Y14 Phosphorylation
K15 Ubiquitination
T21 Phosphorylation Q00535 (CDK5) , P28482 (MAPK1)
R23 Methylation
R30 Methylation
R37 Methylation
S56 Phosphorylation
S103 Phosphorylation
T154 Phosphorylation
K157 Ubiquitination
S215 Phosphorylation
S221 Phosphorylation
K271 Ubiquitination
K362 Acetylation
K370 Ubiquitination
Y372 Phosphorylation
K379 Acetylation
Y389 Phosphorylation
S472 Phosphorylation
T520 Phosphorylation

Research Backgrounds

Function:

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

PTMs:

O-glycosylated.

Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.

Ubiquitinated in the presence of TRIM2 and UBE2D1.

Subunit Structure:

Interacts with ARHGEF28. Interacts with TRIM2.

Family&Domains:

The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.

Belongs to the intermediate filament family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

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