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Product Info

WB 1:3000-1:10000, IF/ICC: 1:200, IP 1:100-1:500
*The optimal dilutions should be determined by the end user.
Monoclonal [T113]
His-Tag Mouse Monoclonal antibody detects endogenous levels of internal, C-terminal, or N-terminal 6His -tagged proteins.
Cite Format: Affinity Biosciences Cat# T0009, RRID:AB_2839416.
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


his tag



A synthetic peptide HHHHHH coupled to KLH.



1). Tan H et al. Identification of human LDHC4 as a potential target for anticancer drug discovery. Acta Pharm Sin B 2022 May;12(5):2348-2357. (PubMed: 35646544) [IF=14.903]

2). Liu X et al. The 1316T> C missenses mutation in MTHFR contributes to MTHFR deficiency by targeting MTHFR to proteasome degradation. Aging (Albany NY) 2020 Dec 3;12. (PubMed: 33290257) [IF=5.955]

3). Xu CJ et al. Short-term high-fat diet favors the appearances of apoptosis and gliosis by activation of ERK1/2/p38MAPK pathways in brain. Aging (Albany NY) 2021 Oct 7;13(19):23133-23148. (PubMed: 34620734) [IF=5.955]

4). Luo X et al. Recombinant expression a novel fibronectin—collage fusion peptide modulating stem cell stemness via integrin β3. Appl Microbiol Biotechnol 2022 May;106(9-10):3765-3776. (PubMed: 35590080) [IF=5.560]

5). Ge S et al. Canine Parvovirus is diagnosed and neutralized by chicken IgY-scFv generated against the virus capsid protein. Vet Res 2020 Sep 3;51(1):110. (PubMed: 32883344) [IF=3.829]

Application: WB    Species:    Sample: CRFK cells

Figure 7 |Neutralization efect of obtained scFv to CPV. A scFv gene was ligated pcMV-3 vector. B Immunofuorescence analysis of scFv transfected CRFK cells. control: CRFK cells of pcMV-3 without scFv, scFv: CRFK cells of pcMV-3 with scFv, DAPI: staining nuclei. C western blot detection of scFv transfected CRFK cells, control represent empty pcMV-3 transfected CRFK cells.

6). Duan Z et al. Chicken bromodomain-containing protein 2 interacts with the Newcastle disease virus matrix protein and promotes viral replication. Vet Res 2020 Sep 22;51(1):120. (PubMed: 32962745) [IF=3.829]

Application: WB    Species:    Sample:

Figure 1| Identifcation of the interaction between the NDV M protein and chBRD2 protein.C A Coomassie stained gel showing the bacterial expression purifed proteins. The His-tagged chBRD2 (His-chBRD2), His tag,GST-tagged M (GST-M), and GST tag were expressed in E. coli BL21 (DE3), and the soluble His-chBRD2 and His tag or GST-M and GST tag were purifed on Ni–NTA His*Bind Resin or Glutathione-Sepharose 4B beads, respectively. The bacterial expression purifed proteins were detected by SDS-PAGE along with Coomassie blue staining. Identifcation of the interaction between the M protein and chBRD2 protein by His pull-down assay (D) and GST pull-down assay (E).

7). Wang JL et al. Nogo-A-Δ20/EphA4 interaction antagonizes apoptosis of neural stem cells by integrating p38 and JNK MAPK signaling. J Mol Histol 2021 Jun;52(3):521-537. (PubMed: 33555537) [IF=3.156]

8). A sensitive and reversible staining of proteins on blot membranes.

9). Generation and Evaluation of Chicken IgY-scFv for the Purposes of CPV Diagnosis and Therapy.

10). Study on β-Chitosan against the binding of SARS-CoV-2S-RBD/ACE2.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.