GST-Tag Antibody - #T0007
(7)
(3)
| Product: | GST-Tag Antibody |
| Catalog: | T0007 |
| Description: | Mouse monoclonal antibody to GST-Tag |
| Application: | WB ELISA |
| Reactivity: | All |
| Uniprot: | |
| RRID: | AB_2839414 |
Product Info
Source:
Mouse
Application:
WB 1:3000-1:10000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
All
Clonality:
Monoclonal [T571]
Specificity:
GST-Tag Mouse Monoclonal antibody detects endogenous levels of C-terminal, internal, and N-terminal GST-tagged proteins.
RRID:
AB_2839414
Cite Format: Affinity Biosciences Cat# T0007, RRID:AB_2839414.
Cite Format: Affinity Biosciences Cat# T0007, RRID:AB_2839414.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Immunogens
Immunogen:
Full-length GST protein.
References
1). miR-506 enhances the sensitivity of human colorectal cancer cells to oxaliplatin by suppressing MDR1/P-gp expression. CELL PROLIFERATION, 2017
(PubMed: 28217977)
[IF=8.5]
Application: WB Species: human Sample:
FIGURE 3 miR-506 down-regulated MDR1/P-gp expression in HCT116-OxR. A, The mRNA level of MDR1 was decreased after transfection with the miR-506 mimic of the relative chemoresistant genes as demonstrated by qRT-PCR. B, The protein level of MDR1 was decreased after transfection with the miR-506 mimic of the relative chemoresistant proteins as demonstrated by Western blot. C, Expression of P-gp detected by immunofluorescence staining.HCT116-OxR-miR-506 cells showed low levels of fluorescent staining of P-gp, whereas maximal staining of P-gp was observed in HCT116-OxR cells, readily distinguished from background. Zoom: 200×. *P<.05)
2). Plant AFC2 kinase desensitizes thermomorphogenesis through modulation of alternative splicing. iScience, 2022
(PubMed: 35345463)
[IF=5.8]
3). Hepatoprotective and Anti-Oxidative Effects of Total Flavonoids From Qu Zhi Qiao (Fruit of Citrus Paradisi cv.Changshanhuyou) on Nonalcoholic Steatohepatitis In Vivo and In Vitro Through Nrf2-ARE Signaling Pathway. Frontiers in Pharmacology, 2020
(PubMed: 32390839)
[IF=5.6]
Application: WB Species: mouse Sample: liver
FIGURE 5 | Expression of the targeted proteins in mice liver. Values are shown as mean ± SD (n = 8). Data (mean ± SD) with different case letters are statistically different with each other at LSD multiple comparisons, which with red letters differed significantly as compared to model group level. uppercase letter p < 0.01 vs.another group.
4). Chicken bromodomain-containing protein 2 interacts with the Newcastle disease virus matrix protein and promotes viral replication. Veterinary Research, 2020
(PubMed: 32962745)
[IF=4.4]
Application: WB Species: Sample:
Figure 1| Identifcation of the interaction between the NDV M protein and chBRD2 protein.C A Coomassie stained gel showing the bacterial expression purifed proteins. The His-tagged chBRD2 (His-chBRD2), His tag,GST-tagged M (GST-M), and GST tag were expressed in E. coli BL21 (DE3), and the soluble His-chBRD2 and His tag or GST-M and GST tag were purifed on Ni–NTA His*Bind Resin or Glutathione-Sepharose 4B beads, respectively. The bacterial expression purifed proteins were detected by SDS-PAGE along with Coomassie blue staining. Identifcation of the interaction between the M protein and chBRD2 protein by His pull-down assay (D) and GST pull-down assay (E).
5). Molecular and Structural Basis of Receptor Binding and Signaling of a Fish Type I IFN with Three Disulfide Bonds. The Journal of Immunology, 2022
(PubMed: 35906001)
[IF=4.4]
6). Structural and Functional Characterization of a Fish Type I Subgroup d IFN Reveals Its Binding to Receptors. Journal of immunology (Baltimore, Md. : 1950), 2024
(PubMed: 38345351)
[IF=4.4]
7). Phos-tag-based non-radioactive protocols for monitoring Arabidopsis kinase activities in vitro. STAR Protocols, 2022
(PubMed: 36149791)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
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