Flag-Tag Antibody - #T0003
(24)
(9)
Product Info
Source:
Mouse
Application:
WB 1:3000-1:10000, IF/ICC: 1:200, IP 1:200, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
All
Clonality:
Monoclonal [T177]
RRID:
AB_2839412
Cite Format: Affinity Biosciences Cat# T0003, RRID:AB_2839412.
Cite Format: Affinity Biosciences Cat# T0003, RRID:AB_2839412.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
DDDDK epitope tag; DDDK; ddk; DYKDDDDK; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG tag antibody
Immunogens
Immunogen:
A synthetic peptide DYKDDDDK coupled to KLH.
Description:
FLAG tag Mouse mAb is part of the series of Tag antibodies, the excellent quality in the research. FLAG tag antibody is a highly sensitive and affinity PAB applicable to FLAG-tagged fusion protein detection. FLAG tag antibody can detect FLAG tags in internal, C-terminal, or N-terminal recombinant proteins.
References
1). Young LINE-1 transposon 5′ UTRs marked by elongation factor ELL3 function as enhancers to regulate naïve pluripotency in embryonic stem cells. Nature Cell Biology, 2023
(PubMed: 37591949)
[IF=17.3]
2). Psychologic Stress Drives Progression of Malignant Tumors via DRD2/HIF1α Signaling. CANCER RESEARCH, 2021
(PubMed: 34321238)
[IF=12.5]
Application: WB Species: mouse Sample:
Fig. 4 |DRD2 overexpression inhibited the ubiquitin-dependent degradation of HIF1α. N, Effect of DRD2 on the content of ubiquitin binding to HIF1α. DRD2-overexpressing cells treated with MG132, and cellular extracts were prepared for co-IP assay with anti–HIF1α antibody, followed by Western blotting detection of ubiquitin. siRNA, small interfering RNA. ns, nonsignificant; *, P < 0.05; **, P < 0.01
3). Micheliolide Ameliorates Diabetic Kidney Disease by Inhibiting Mtdh-mediated Renal Inflammation in Type 2 Diabetic db/db Mice. PHARMACOLOGICAL RESEARCH, 2019
(PubMed: 31669149)
[IF=9.1]
4). WWP2 drives the progression of gastric cancer by facilitating the ubiquitination and degradation of LATS1 protein. Cell Communication and Signaling, 2023
(PubMed: 36803368)
[IF=8.4]
5). MCPIP1-mediated NFIC alternative splicing inhibits proliferation of triple-negative breast cancer via cyclin D1-Rb-E2F1 axis. Cell Death & Disease, 2021
(PubMed: 33824311)
[IF=8.1]
6). SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis. FREE RADICAL BIOLOGY AND MEDICINE, 2019
(PubMed: 30703481)
[IF=7.1]
Application: WB Species: human Sample: HEK293A cells
Fig. 6.| SIRT5 promotes ccRCC cell proliferation by interacting with and desuccinylating SDHA. (A-B) ACHN cells were transfected with scramble shRNA or SIRT5 shRNA plasmid, SDHA was precipitated and its succinylation level was probed by anti-pan-succinylation antibody. SIRT5 silencing led to hypersuccinylation of SDHA in ccRCC cells and increased activity of SDHA. (C-E) SIRT5 silencing decreased cellular NADPH/NADP+and GSH/GSSG ratio and increased cellular ROS level. (F) Co-IP of SIRT5 and FLAG-tagged WT SDHA in HEK293A cells revealed SIRT5 coimmunoprecipitated with SDHA. (G-J) SIRT5 silencing repressed ACHN and 769-P cell proliferation and colony formation. Methods followed Fig. 5G-J.
7). Ablation of Cbl-b and c-Cbl in dendritic cells causes spontaneous liver cirrhosis via altering multiple properties of CD103+ cDC1s. Cell Death Discovery, 2022
(PubMed: 35354799)
[IF=7.0]
Application: WB Species: human Sample: HEK293T cells
Fig. 6 |Cbl-b and c-Cbl ubiquitinate different lysine sites of STAT5. The ubiquitination of STAT5a (A) and STAT5b (B) by Cbl-b and c-Cbl in HEK293T cells.
8). circFBXW7 Inhibits Malignant Progression by Sponging miR-197-3p and Encoding a 185-aa Protein in Triple-Negative Breast Cancer. Molecular Therapy-Nucleic Acids, 2019
(PubMed: 31536884)
[IF=6.5]
Application: WB Species: human Sample: BT549 cells
Figure 6.| FBXW7-185aa, Encoded by circFBXW7, Inhibited the Proliferation and Metastasis of TNBC Cells through Downregulating c-Myc Expression.s. (H)FBXW7-185aa-FLAG-overexpressing BT549 cells were cotransfected with sh-FBXW7 or USP28 vectors. The FBXW7-185aaFLAG, FBXW7, USP28, and c-Myc expression levels were quantified by western blot analysis.
9). Lysosomal K+ channel TMEM175 promotes apoptosis and aggravates symptoms of Parkinson's disease. EMBO reports, 2022
(PubMed: 35913019)
[IF=6.5]
10). Mafa Enables Pdx1 to Effectively Convert Pancreatic Islet Progenitors and Committed Islet α-Cells Into β-Cells In Vivo. DIABETES, 2017
(PubMed: 28223284)
[IF=6.2]
Application: IF/ICC Species: mouse Sample: islet cells
Supplemental Figure 1| Pdx1 flag is expressed in most P0.5 and 6W Ngn3-Cre;CAT-Pdx1 islet cells. Flag staining (red) was observed in most of Ngn3-Cre;CAT-Pdx1 insulin + (blue) and glucagon + (green) cells at P0.5 and 6W; the Crepenetrance rate was a maintained at approximately 95%. Scale bars: 50 mm.
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