Flag-Tag Antibody - #T0003
(22)
(10)
Product Info
Source:
Mouse
Application:
WB 1:3000-1:10000, IF/ICC: 1:200, IP 1:200, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
All
Clonality:
Monoclonal [T177]
RRID:
AB_2839412
Cite Format: Affinity Biosciences Cat# T0003, RRID:AB_2839412.
Cite Format: Affinity Biosciences Cat# T0003, RRID:AB_2839412.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
DDDDK epitope tag; DDDK; ddk; DYKDDDDK; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG tag antibody
Immunogens
Immunogen:
A synthetic peptide DYKDDDDK coupled to KLH.
Description:
FLAG tag Mouse mAb is part of the series of Tag antibodies, the excellent quality in the research. FLAG tag antibody is a highly sensitive and affinity PAB applicable to FLAG-tagged fusion protein detection. FLAG tag antibody can detect FLAG tags in internal, C-terminal, or N-terminal recombinant proteins.
References
1). Young LINE-1 transposon 5′ UTRs marked by elongation factor ELL3 function as enhancers to regulate naïve pluripotency in embryonic stem cells. Nature Cell Biology, 2023
(PubMed: 37591949)
[IF=21.3]
2). Psychologic Stress Drives Progression of Malignant Tumors via DRD2/HIF1α Signaling. CANCER RESEARCH, 2021
(PubMed: 34321238)
[IF=11.2]
Application: WB Species: mouse Sample:
Fig. 4 |DRD2 overexpression inhibited the ubiquitin-dependent degradation of HIF1α. N, Effect of DRD2 on the content of ubiquitin binding to HIF1α. DRD2-overexpressing cells treated with MG132, and cellular extracts were prepared for co-IP assay with anti–HIF1α antibody, followed by Western blotting detection of ubiquitin. siRNA, small interfering RNA. ns, nonsignificant; *, P < 0.05; **, P < 0.01
3). Micheliolide Ameliorates Diabetic Kidney Disease by Inhibiting Mtdh-mediated Renal Inflammation in Type 2 Diabetic db/db Mice. PHARMACOLOGICAL RESEARCH, 2019
(PubMed: 31669149)
[IF=9.3]
4). MCPIP1-mediated NFIC alternative splicing inhibits proliferation of triple-negative breast cancer via cyclin D1-Rb-E2F1 axis. Cell Death & Disease, 2021
(PubMed: 33824311)
[IF=9.0]
5). circFBXW7 Inhibits Malignant Progression by Sponging miR-197-3p and Encoding a 185-aa Protein in Triple-Negative Breast Cancer. Molecular Therapy-Nucleic Acids, 2019
(PubMed: 31536884)
[IF=8.8]
Application: WB Species: human Sample: BT549 cells
Figure 6.| FBXW7-185aa, Encoded by circFBXW7, Inhibited the Proliferation and Metastasis of TNBC Cells through Downregulating c-Myc Expression.s. (H)FBXW7-185aa-FLAG-overexpressing BT549 cells were cotransfected with sh-FBXW7 or USP28 vectors. The FBXW7-185aaFLAG, FBXW7, USP28, and c-Myc expression levels were quantified by western blot analysis.
6). WWP2 drives the progression of gastric cancer by facilitating the ubiquitination and degradation of LATS1 protein. Cell Communication and Signaling, 2023
(PubMed: 36803368)
[IF=8.4]
7). Mafa Enables Pdx1 to Effectively Convert Pancreatic Islet Progenitors and Committed Islet α-Cells Into β-Cells In Vivo. DIABETES, 2017
(PubMed: 28223284)
[IF=7.7]
Application: IF/ICC Species: mouse Sample: islet cells
Supplemental Figure 1| Pdx1 flag is expressed in most P0.5 and 6W Ngn3-Cre;CAT-Pdx1 islet cells. Flag staining (red) was observed in most of Ngn3-Cre;CAT-Pdx1 insulin + (blue) and glucagon + (green) cells at P0.5 and 6W; the Crepenetrance rate was a maintained at approximately 95%. Scale bars: 50 mm.
8). SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis. FREE RADICAL BIOLOGY AND MEDICINE, 2019
(PubMed: 30703481)
[IF=7.4]
Application: WB Species: human Sample: HEK293A cells
Fig. 6.| SIRT5 promotes ccRCC cell proliferation by interacting with and desuccinylating SDHA. (A-B) ACHN cells were transfected with scramble shRNA or SIRT5 shRNA plasmid, SDHA was precipitated and its succinylation level was probed by anti-pan-succinylation antibody. SIRT5 silencing led to hypersuccinylation of SDHA in ccRCC cells and increased activity of SDHA. (C-E) SIRT5 silencing decreased cellular NADPH/NADP+and GSH/GSSG ratio and increased cellular ROS level. (F) Co-IP of SIRT5 and FLAG-tagged WT SDHA in HEK293A cells revealed SIRT5 coimmunoprecipitated with SDHA. (G-J) SIRT5 silencing repressed ACHN and 769-P cell proliferation and colony formation. Methods followed Fig. 5G-J.
9). Ablation of Cbl-b and c-Cbl in dendritic cells causes spontaneous liver cirrhosis via altering multiple properties of CD103+ cDC1s. Cell Death Discovery, 2022
(PubMed: 35354799)
[IF=7.0]
Application: WB Species: human Sample: HEK293T cells
Fig. 6 |Cbl-b and c-Cbl ubiquitinate different lysine sites of STAT5. The ubiquitination of STAT5a (A) and STAT5b (B) by Cbl-b and c-Cbl in HEK293T cells.
10). CHCHD2 Thr61Ile mutation impairs F1F0-ATPase assembly in in vitro and in vivo models of Parkinson’s disease. Neural Regeneration Research, 2024
(PubMed: 37488867)
[IF=6.1]
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For Research Use Only.
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