C-Myc-Tag Antibody - #T0001
(4)
(3)
| Product: | C-Myc-Tag Antibody |
| Catalog: | T0001 |
| Description: | Mouse monoclonal antibody to C-Myc-Tag |
| Application: | WB IP ELISA |
| Reactivity: | All |
| Uniprot: | |
| RRID: | AB_2839411 |
Product Info
Source:
Mouse
Application:
WB 1:3000-1:10000, IF/ICC: 1:200, IP 1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
All
Clonality:
Monoclonal [T335]
Specificity:
N/A.
RRID:
AB_2839411
Cite Format: Affinity Biosciences Cat# T0001, RRID:AB_2839411.
Cite Format: Affinity Biosciences Cat# T0001, RRID:AB_2839411.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Immunogens
Immunogen:
A synthetic peptide EQKLISEEDL coupled to KLH.
Description:
c-Myc-tag antibody is part of the Tag series of antibodies, the best quality in the research. The immunogen of c-Myc tag antibody is a synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH. C-Myc tag antibody is suitable for detecting the expression level of c-Myc or its fusion proteins where the c-Myc tag is terminal or internal.
References
1). Mutation of Basic Residues R283, R286, and K288 in the Matrix Protein of Newcastle Disease Virus Attenuates Viral Replication and Pathogenicity. International Journal of Molecular Sciences, 2023
(PubMed: 36674496)
[IF=5.6]
2). Sls1 and Mtf2 mediate the assembly of the Mrh5C complex required for activation of cox1 mRNA translation. The Journal of biological chemistry, 2024
(PubMed: 38499152)
[IF=4.8]
Application: WB Species: Mouse Sample: WT cells
Figure 2. Mtf2 and Sls1 are required for the assembly of Mrh5C.A and B, loss of Mtf2 disrupts Mrh5C. Mitochondrial extracts prepared from WT cells expressing untagged Sls1 (negative control) or Sls1-FLAG (positive control), and Δmtf2 cells expressing Sls1-FLAG were subjected to anti-FLAG co-immunoprecipitation (A). Mitochondrial extracts prepared from WT cells expressing untagged Mrh5 or Mrh5-Myc, and Δmtf2 cells expressing Mrh5-Myc were subjected to anti-Myc co-immunoprecipitation (B). C, loss of Sls1 disrupts Mrh5C. WT cells expressing untagged Mrh5 or Mrh5-Myc and Δmtf2 cells expressing Mrh5-Myc were subjected to anti-Myc co-immunoprecipitation. D, loss of Mrh5 does not affect interactions among other members of Mrh5C. WT cells expressing untagged Sls1 or Sls1-FLAG, and Δmrh5 cells expressing Sls1-FLAG were subjected to anti-FLAG co-immunoprecipitation. E, loss of Ppr4 does not affect interactions among other members of Mrh5C. WT cells expressing untagged Sls1 or Sls1-FLAG, and Δppr4 cells expressing Sls1-FLAG were subjected to anti-FLAG co-immunoprecipitation. F, Mtf2 and Sls1 form a complex independently of Mrh5 and Ppr4. WT cells expressing untagged Sls1 or Sls1-FLAG, and Δmrh5Δppr4 cells expressing Sls1-FLAG were subjected to anti-FLAG co-immunoprecipitation. Mitochondrial extracts (IN) and immunoprecipitates (IP) were analyzed by immunoblotting using specific anti-tag Abs. Genes encoding the Mrh5C subunits were endogenously tagged to facilitate their detection.
3). Chicken bromodomain-containing protein 2 interacts with the Newcastle disease virus matrix protein and promotes viral replication. Veterinary Research, 2020
(PubMed: 32962745)
[IF=4.4]
Application: WB Species: Sample:
Figure 2| Characterization of the binding domains between the NDV M protein and chBRD2 protein. B Mapping the binding domain of the chBRD2 protein for the NDV M protein. Upper panel, schematic representation of the full-length and truncation mutants of the Myc-tagged chBRD2 protein. Lower panel, Myc-tagged chBRD2 or its truncation mutants (Input) interacting with HA-M was detected by western blot.
4). Schizosaccharomyces pombe MAP kinase Sty1 promotes survival of Δppr10 cells with defective mitochondrial protein synthesis. The international journal of biochemistry & cell biology, 2022
(PubMed: 36174923)
[IF=4.0]
Restrictive clause
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For Research Use Only.
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