FOXD3 Antibody - #BF0061

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Product: FOXD3 Antibody
Catalog: BF0061
Description: Mouse monoclonal antibody to FOXD3
Application: WB IHC ELISA
Reactivity: Human, Mouse, Monkey
Mol.Wt.: 50kDa; 48kD(Calculated).
Uniprot: Q9UJU5
RRID: AB_2833870

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 50ul $250 In stock
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Lead Time: Same day delivery

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Local distributors
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Clonality:
Monoclonal [AFB1846]
Specificity:
FOXD3 antibody detects endogenous levels of total FOXD3.
RRID:
AB_2833870
Cite Format: Affinity Biosciences Cat# BF0061, RRID:AB_2833870.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AIS1; forkhead box D3; Forkhead box protein D3; foxd3; FOXD3_HUMAN; Genesis; HFH2; HNF3/FH transcription factor genesis; VAMAS2;

Immunogens

Immunogen:

Purified recombinant fragment of human FOXD3 expressed in E. Coli.

Uniprot:

Q9UJU5(FOXD3_HUMAN) >>Visit HPA database.

Gene(ID):
FOXD3(27022)
Expression:
Q9UJU5 FOXD3_HUMAN:

Expressed in chronic myeloid leukemia, Jurkat T-cell leukemia and teratocarcinoma cell lines, but not in any other cell lines or normal tissues examined.

Description:
FoxD3 is a member of the Forkhead Box family and is characterized by a winged-helix DNA-binding structure and the important role it plays in embryonic development . This transcriptional regulator is required for the maintenance of pluripotency in the pre-implantation and peri-implantation stages of mouse embryonic development and is also required for trophoblast formation.
Sequence:
MTLSGGGSASDMSGQTVLTAEDVDIDVVGEGDDGLEEKDSDAGCDSPAGPPELRLDEADEVPPAAPHHGQPQPPHQQPLTLPKEAAGAGAGPGGDVGAPEADGCKGGVGGEEGGASGGGPGAGSGSAGGLAPSKPKNSLVKPPYSYIALITMAILQSPQKKLTLSGICEFISNRFPYYREKFPAWQNSIRHNLSLNDCFVKIPREPGNPGKGNYWTLDPQSEDMFDNGSFLRRRKRFKRHQQEHLREQTALMMQSFGAYSLAAAAGAAGPYGRPYGLHPAAAAGAYSHPAAAAAAAAAAALQYPYALPPVAPVLPPAVPLLPSGELGRKAAAFGSQLGPGLQLQLNSLGAAAAAAGTAGAAGTTASLIKSEPSARPSFSIENIIGGGPAAPGGSAVGAGVAGGTGGSGGGSTAQSFLRPPGTVQSAALMATHQPLSLSRTTATIAPILSVPLSGQFLQPAASAAAAAAAAAQAKWPAQ

PTMs - Q9UJU5 As Substrate

Site PTM Type Enzyme
S40 Phosphorylation
S46 Phosphorylation
Y214 Phosphorylation
S229 Phosphorylation

Research Backgrounds

Function:

Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in chronic myeloid leukemia, Jurkat T-cell leukemia and teratocarcinoma cell lines, but not in any other cell lines or normal tissues examined.

Subunit Structure:

Interacts with POU5F1.

Restrictive clause

 

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