KLF4 Antibody - #BF0440
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# BF0440, RRID:AB_2833844.
Fold/Unfold
Endothelial Kruppel like zinc finger protein; Epithelial zinc finger protein EZF; EZF; GKLF; gut Kruppel-like factor; Gut-enriched krueppel-like factor; KLF; KLF4; KLF4_HUMAN; Krueppel-like factor 4; Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor); Kruppel like factor 4 (gut);
Immunogens
Purified recombinant fragment of human KLF4 expressed in E. Coli.
- O43474 KLF4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRQPPGESDMAVSDALLPSFSTFASGPAGREKTLRQAGAPNNRWREELSHMKRLPPVLPGRPYDLAAATVATDLESGGAGAACGGSNLAPLPRRETEEFNDLLDLDFILSNSLTHPPESVAATVSSSASASSSSSPSSSGPASAPSTCSFTYPIRAGNDPGVAPGGTGGGLLYGRESAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQPQPPGGGLMGKFVLKASLSAPGSEYGSPSVISVSKGSPDGSHPVVVAPYNGGPPRTCPKIKQEAVSSCTHLGAGPPLSNGHRPAAHDFPLGRQLPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYPSFLPDQMQPQVPPLHYQGQSRGFVARAGEPCVCWPHFGTHGMMLTPPSSPLELMPPGSCMPEEPKPKRGRRSWPRKRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDRAFSRSDHLALHMKRHF
PTMs - O43474 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K32 | Ubiquitination | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| K278 | Sumoylation | Uniprot | |
| T316 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| T429 | Phosphorylation | Uniprot | |
| S444 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| K448 | Sumoylation | Uniprot | |
| K448 | Ubiquitination | Uniprot | |
| T453 | Phosphorylation | Uniprot | |
| T455 | Phosphorylation | Uniprot |
Research Backgrounds
Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Nucleus.
Interacts with POU5F1/OCT4 and SOX2 (By similarity). Interacts with MUC1 (via the C-terminal domain). Interacts with MEIS2 isoform 4 and PBX1 isoform PBX1a. Interacts with ZNF296 (By similarity). Interacts with GLIS1.
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Belongs to the krueppel C2H2-type zinc-finger protein family.
Research Fields
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
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