TCF3 Antibody - #BF0632

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Product: TCF3 Antibody
Catalog: BF0632
Description: Mouse monoclonal antibody to TCF3
Application: WB ELISA FACS
Reactivity: Human
Mol.Wt.: 67.6kDa; 68kD(Calculated).
Uniprot: P15923
RRID: AB_2833768

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
ELISA 1:10000, WB 1:500-1:2000, FCM 1:200-1:400
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFB1744]
Specificity:
TCF3 antibody detects endogenous levels of total TCF3.
RRID:
AB_2833768
Cite Format: Affinity Biosciences Cat# BF0632, RRID:AB_2833768.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AGM8; bHLHb21; Class B basic helix-loop-helix protein 21; E12; E2A; E2A immunoglobulin enhancer binding factors E12/E47; E47; Helix loop helix protein HE47; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; ITF1; Kappa-E2-binding factor; MGC129647; MGC129648; Negative vitamin D response element binding protein; NOL1-TCF3 fusion; TCF-3; Tcf3; TFE2_HUMAN; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); Transcription factor 3; transcription factor 3 variant 3; Transcription factor E2-alpha; Transcription factor ITF-1; VDIR; VDR interacting repressor; vitamin D receptor-interacting repressor;

Immunogens

Immunogen:

Purified recombinant fragment of human TCF3 expressed in E. Coli.

Uniprot:

P15923(TFE2_HUMAN) >>Visit HPA database.

Gene(ID):
TCF3(6929)
Description:
E2A is a transcription factor that plays major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Heterodimers between E2A and tissue-specific basic helix-loop-helix (bHLH) Dimers bind DNA on E-box motifs: 5'- CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Deletions in E2A have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Two alternatively spliced human isoforms have been described.
Sequence:
MNQPQRMAPVGTDKELSDLLDFSMMFPLPVTNGKGRPASLAGAQFGGSGLEDRPSSGSWGSGDQSSSSFDPSRTFSEGTHFTESHSSLSSSTFLGPGLGGKSGERGAYASFGRDAGVGGLTQAGFLSGELALNSPGPLSPSGMKGTSQYYPSYSGSSRRRAADGSLDTQPKKVRKVPPGLPSSVYPPSSGEDYGRDATAYPSAKTPSSTYPAPFYVADGSLHPSAELWSPPGQAGFGPMLGGGSSPLPLPPGSGPVGSSGSSSTFGGLHQHERMGYQLHGAEVNGGLPSASSFSSAPGATYGGVSSHTPPVSGADSLLGSRGTTAGSSGDALGKALASIYSPDHSSNNFSSSPSTPVGSPQGLAGTSQWPRAGAPGALSPSYDGGLHGLQSKIEDHLDEAIHVLRSHAVGTAGDMHTLLPGHGALASGFTGPMSLGGRHAGLVGGSHPEDGLAGSTSLMHNHAALPSQPGTLPDLSRPPDSYSGLGRAGATAAASEIKREEKEDEENTSAADHSEEEKKELKAPRARTSPDEDEDDLLPPEQKAEREKERRVANNARERLRVRDINEAFKELGRMCQLHLNSEKPQTKLLILHQAVSVILNLEQQVRERNLNPKAACLKRREEEKVSGVVGDPQMVLSAPHPGLSEAHNPAGHM

PTMs - P15923 As Substrate

Site PTM Type Enzyme
T12 Phosphorylation
K34 Acetylation
S39 Phosphorylation Q9P286 (PAK5)
S48 Phosphorylation
R105 Methylation
Y108 Phosphorylation
S110 Phosphorylation
S134 Phosphorylation
S139 Phosphorylation Q16539 (MAPK14) , P11802 (CDK4) , Q15759 (MAPK11) , P24941 (CDK2)
S141 Phosphorylation
Y149 Phosphorylation
Y150 Phosphorylation
Y153 Phosphorylation
S156 Phosphorylation
K171 Acetylation
S245 Phosphorylation P11802 (CDK4) , P24941 (CDK2)
S341 Phosphorylation Q02779 (MAP3K10)
S345 Phosphorylation
S346 Phosphorylation
S350 Phosphorylation
S351 Phosphorylation
S352 Phosphorylation Q02779 (MAP3K10)
S354 Phosphorylation
T355 Phosphorylation Q02779 (MAP3K10)
S359 Phosphorylation Q02779 (MAP3K10)
T366 Phosphorylation
S367 Phosphorylation
R371 Methylation
S379 Phosphorylation Q02779 (MAP3K10)
S381 Phosphorylation
Y382 Phosphorylation
K392 Sumoylation
T411 Phosphorylation
K498 Sumoylation
K502 Sumoylation
S509 Phosphorylation
S514 Phosphorylation
T528 Phosphorylation
S529 Phosphorylation
K570 Ubiquitination
K584 Ubiquitination
K614 Ubiquitination
K625 Ubiquitination

Research Backgrounds

Function:

Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region.

PTMs:

Phosphorylated following NGF stimulation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Forms a heterodimer with ASH1 and TWIST2. Isoform E12 interacts with RALGAPA1 and FIGLA. Efficient DNA binding requires dimerization with another bHLH protein. Component of a nuclear TAL-1 complex composed at least of CBFA2T3, LDB1, TAL1 and TCF3. Interacts with NEUROD2, PTF1A and TGFB1I1. Forms a heterodimer with MYOG; heterodimerization enhances MYOG DNA-binding and transcriptional activities (By similarity). Homodimer. Heterodimer. Forms a heterodimer with NEUROD1; the heterodimer is inhibited in presence of ID2, but not NR0B2, to E-box element. Interacts with EP300 and UBE2I. Interacts with BHLHA9. Forms a heterodimer with ATOH8; repress transcription of TCF3 and TCF3/NEUROG3 dimer-induced transactivation of E box-dependent promoters (By similarity).

Family&Domains:

the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

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