BDH1 Antibody - #BF0318
(2)
(2)
| Product: | BDH1 Antibody |
| Catalog: | BF0318 |
| Description: | Mouse monoclonal antibody to BDH1 |
| Application: | WB IHC ELISA |
| Cited expt.: | WB |
| Reactivity: | Human, Mouse |
| Mol.Wt.: | 38kDa; 38kD(Calculated). |
| Uniprot: | Q02338 |
| RRID: | AB_2833761 |
Product Info
Source:
Mouse
Application:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Clonality:
Monoclonal [AFB1737]
Specificity:
BDH1 antibody detects endogenous levels of total BDH1.
RRID:
AB_2833761
Cite Format: Affinity Biosciences Cat# BF0318, RRID:AB_2833761.
Cite Format: Affinity Biosciences Cat# BF0318, RRID:AB_2833761.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
3 hydroxybutyrate dehydrogenase (heart, mitochondrial); 3-hydroxybutyrate dehydrogenase; BDH; BDH_HUMAN; BDH1; D beta hydroxybutyrate dehydrogenase, mitochondrial; D-beta-hydroxybutyrate dehydrogenase; mitochondrial;
Immunogens
Immunogen:
Purified recombinant fragment of human BDH1 expressed in E. Coli.
Uniprot:
Gene(ID):
Description:
BDH1 (3-hydroxybutyrate dehydrogenase, type 1), it is a member of the short-chain dehydrogenase/reductase gene family. This protien forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. It catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism.
Sequence:
- Q02338 BDH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLATRLSRPLSRLPGKTLSACDRENGARRPLLLGSTSFIPIGRRTYASAAEPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLNSDRLRTVQLNVCSSEEVEKVVEIVRSSLKDPEKGMWGLVNNAGISTFGEVEFTSLETYKQVAEVNLWGTVRMTKSFLPLIRRAKGRVVNISSMLGRMANPARSPYCITKFGVEAFSDCLRYEMYPLGVKVSVVEPGNFIAATSLYSPESIQAIAKKMWEELPEVVRKDYGKKYFDEKIAKMETYCSSGSTDTSPVIDAVTHALTATTPYTRYHPMDYYWWLRMQIMTHLPGAISDMIYIR
Research Backgrounds
Subcellular Location:
Mitochondrion inner membrane. Mitochondrion matrix.
Family&Domains:
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Research Fields
· Metabolism > Lipid metabolism > Synthesis and degradation of ketone bodies.
· Metabolism > Carbohydrate metabolism > Butanoate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
References
1). β-Hydroxybutyrate suppresses M1 macrophage polarization through β-hydroxybutyrylation of the STAT1 protein. Cell death & disease, 2024
(PubMed: 39627223)
[IF=8.1]
Application: WB Species: Mouse Sample: RAW264.7 cells
Fig. 4: Effects of β-OHB treatment on the regulation of M1 macrophage polarization. RAW264.7 cells were first treated with 10 mM β-OHB for 24 h, then cultured under β-OHB-free culture conditions for 24 and 48 h, and stimulated with 100 ng/ml LPS for 24 h. A Immunoblotting analysis of the Kbhb modification in the RAW264.7 cells (n = 3/group). B–D RT-qPCR analysis of IL-6, IL-12, and iNOS mRNA expression levels in the RAW264.7 cells (n = 3/group). The 9-week-old male C57BL/6 mice were orally administered 3 mg/g KE per day for 3d, and their BMCs were obtained and cultured with LCS for 7d to induce BMC differentiation into BMDMs. E, F RT-qPCR analysis of F4/80 and CD11b mRNA expression levels in the BMDMs (n = 3/group). G Immunoblotting analysis of the Kbhb modification in the BMCs treated with LCS at the indicated times (n = 3/group). Partial BMDMs were stimulated with 100 ng/ml LPS for 24 h. H–J RT-qPCR analysis of IL-6, IL-12, and iNOS mRNA expression levels in the BMDMs (n = 3/group). K BDH1 protein expression in mice liver lysates, HepG2 cells, BMDMs, and PMs. L Lack of BDH1 expression leads to β-OHB accumulation in macrophages, which may induce its Kbhb modification. BMDMs were transfected with either Ad-normal control (NC) or Ad-BDH1, then treated with 10 mM β-OHB for 24 h, followed by stimulation with 100 ng/ml LPS for an additional 24 h. M The intracellular β-OHB levels were measured (n = 3/group). N Immunoblotting analysis of the Kbhb modification in the BMDMs (n = 3/group). O–Q RT-qPCR analysis of IL-6, IL-12, and iNOS mRNA expression levels in the BMDMs (n = 3/group). R, S FCM analysis of the proportion of M1 phenotype (iNOS+/F4/80+) mouse BMDMs (n = 3/group). Data are presented as the mean ± standard deviation. *p
2). 3-Hydroxybutyrate Suppresses Colon Cancer Growth by Inducing Intracellular Lactate Accumulation and Oxidative Stress. Journal of proteome research, 2025
(PubMed: 40476748)
[IF=3.8]
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