KRT15 Antibody - #BF0225

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Product: KRT15 Antibody
Catalog: BF0225
Description: Mouse monoclonal antibody to KRT15
Application: WB IHC IF/ICC ELISA FACS
Reactivity: Human, Monkey
Mol.Wt.: 49kDa; 49kD(Calculated).
Uniprot: P19012
RRID: AB_2833743

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000, IF/ICC 1:200-1:1000, FCM 1:200-1:400
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Monkey
Clonality:
Monoclonal [AFB1719]
Specificity:
KRT15 antibody detects endogenous levels of total KRT15.
RRID:
AB_2833743
Cite Format: Affinity Biosciences Cat# BF0225, RRID:AB_2833743.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AI528832; CK 15; CK-15; CK15; Cytokeratin-15; Cytokeratin15; K15; K1C15_HUMAN; K1CO; Ka15; Keratin 15; Keratin 15 basic; Keratin 15 beta; Keratin; Keratin complex 1 acidic gene 15; Keratin type I cytoskeletal 15; Keratin-15; Keratin15; KRT 15; Krt1-15; KRT15; KRTB; KRTL15; Type I cytoskeletal 15; Type I keratin Ka15;

Immunogens

Immunogen:

Purified recombinant fragment of human KRT15 expressed in E. Coli.

Uniprot:

P19012(K1C15_HUMAN) >>Visit HPA database.

Gene(ID):
KRT15(3866)
Expression:
P19012 K1C15_HUMAN:

Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate.

Description:
The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 15 is a type I keratin without a defined type II partner. Keratin 15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. Expression of keratin 15 is downregulated in some hyperproliferating situations, such as psoriasis and hypertrophic scars. Because keratinocytes in psoriasis and hypertrophic scars are activated, it is suggested that keratin 15 expression is not compatible with keratinocyte activation and the keratin 15 gene is downregulated to maintain the activated phenotype.
Sequence:
MTTTFLQTSSSTFGGGSTRGGSLLAGGGGFGGGSLSGGGGSRSISASSARFVSSGSGGGYGGGMRVCGFGGGAGSVFGGGFGGGVGGGFGGGFGGGDGGLLSGNEKITMQNLNDRLASYLDKVRALEEANADLEVKIHDWYQKQTPTSPECDYSQYFKTIEELRDKIMATTIDNSRVILEIDNARLAADDFRLKYENELALRQGVEADINGLRRVLDELTLARTDLEMQIEGLNEELAYLKKNHEEEMKEFSSQLAGQVNVEMDAAPGVDLTRVLAEMREQYEAMAEKNRRDVEAWFFSKTEELNKEVASNTEMIQTSKTEITDLRRTMQELEIELQSQLSMKAGLENSLAETECRYATQLQQIQGLIGGLEAQLSELRCEMEAQNQEYKMLLDIKTRLEQEIATYRSLLEGQDAKMAGIAIREASSGGGGSSSNFHINVEESVDGQVVSSHKREI

PTMs - P19012 As Substrate

Site PTM Type Enzyme
T2 Phosphorylation
S17 Phosphorylation
S22 Phosphorylation
S34 Phosphorylation
S36 Phosphorylation
S41 Phosphorylation
S43 Phosphorylation
S45 Phosphorylation
S53 Phosphorylation
Y60 Phosphorylation
S75 Phosphorylation
S118 Phosphorylation
Y119 Phosphorylation
K122 Acetylation
K122 Ubiquitination
Y141 Phosphorylation
T145 Phosphorylation
S148 Phosphorylation
Y195 Phosphorylation
Y239 Phosphorylation
K241 Sumoylation
K241 Ubiquitination
K300 Methylation
T328 Phosphorylation
S349 Phosphorylation
Y389 Phosphorylation
T405 Phosphorylation
Y406 Phosphorylation
S426 Phosphorylation

Research Backgrounds

Tissue Specificity:

Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate.

Subunit Structure:

Heterotetramer of two type I and two type II keratins. Interacts with NOD2.

Family&Domains:

Belongs to the intermediate filament family.

Research Fields

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

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