Metadherin Antibody - #BF0508

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Product: Metadherin Antibody
Catalog: BF0508
Description: Mouse monoclonal antibody to Metadherin
Application: WB IHC ELISA FACS
Reactivity: Human
Mol.Wt.: 64kDa; 64kD(Calculated).
Uniprot: Q86UE4
RRID: AB_2833740

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000, FCM 1:200-1:400
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFB1716]
Specificity:
Metadherin antibody detects endogenous levels of total Metadherin.
RRID:
AB_2833740
Cite Format: Affinity Biosciences Cat# BF0508, RRID:AB_2833740.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3D3; 3D3/LYRIC; AEG 1; AEG-1; AEG1; Astrocyte elevated gene 1; Astrocyte elevated gene-1 protein; LYRIC; LYRIC/3D3; LYRIC_HUMAN; Lysine rich CEACAM1 associated protein; Lysine rich CEACAM1 co isolated protein; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein; MTDH; Protein LYRIC;

Immunogens

Immunogen:

Purified recombinant fragment of human Metadherin expressed in E. Coli.

Uniprot:

Q86UE4(LYRIC_HUMAN) >>Visit HPA database.

Gene(ID):
MTDH(92140)
Expression:
Q86UE4 LYRIC_HUMAN:

Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.

Description:
Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain; blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.
Sequence:
MAARSWQDELAQQAEEGSARLREMLSVGLGFLRTELGLDLGLEPKRYPGWVILVGTGALGLLLLFLLGYGWAAACAGARKKRRSPPRKREEAAAVPAAAPDDLALLKNLRSEEQKKKNRKKLSEKPKPNGRTVEVAEGEAVRTPQSVTAKQPPEIDKKNEKSKKNKKKSKSDAKAVQNSSRHDGKEVDEGAWETKISHREKRQQRKRDKVLTDSGSLDSTIPGIENTITVTTEQLTTASFPVGSKKNKGDSHLNVQVSNFKSGKGDSTLQVSSGLNENLTVNGGGWNEKSVKLSSQISAGEEKWNSVSPASAGKRKTEPSAWSQDTGDANTNGKDWGRSWSDRSIFSGIGSTAEPVSQSTTSDYQWDVSRNQPYIDDEWSGLNGLSSADPNSDWNAPAEEWGNWVDEERASLLKSQEPIPDDQKVSDDDKEKGEGALPTGKSKKKKKKKKKQGEDNSTAQDTEELEKEIREDLPVNTSKTRPKQEKAFSLKTISTSDPAEVLVKNSQPIKTLPPATSTEPSVILSKSDSDKSSSQVPPILQETDKSKSNTKQNSVPPSQTKSETSWESPKQIKKKKKARRET

PTMs - Q86UE4 As Substrate

Site PTM Type Enzyme
S84 Phosphorylation
K88 Ubiquitination
K107 Ubiquitination
S111 Phosphorylation
K121 Ubiquitination
K125 Ubiquitination
K127 Ubiquitination
T143 Phosphorylation
S146 Phosphorylation
T148 Phosphorylation
K150 Ubiquitination
K170 Acetylation
K174 Acetylation
K174 Ubiquitination
S179 Phosphorylation
S180 Phosphorylation
K185 Ubiquitination
K195 Ubiquitination
S197 Phosphorylation
S214 Phosphorylation
S216 Phosphorylation
S219 Phosphorylation
K248 Ubiquitination
S251 Phosphorylation
S258 Phosphorylation
K261 Methylation
K264 Methylation
S267 Phosphorylation
T268 Phosphorylation
S272 Phosphorylation
S273 Phosphorylation
T280 Phosphorylation
K289 Ubiquitination
K292 Ubiquitination
S294 Phosphorylation
S295 Phosphorylation
S298 Phosphorylation
K303 Ubiquitination
S306 Phosphorylation
S308 Phosphorylation
S311 Phosphorylation
K314 Acetylation
K314 Methylation
K314 Ubiquitination
S323 Phosphorylation
K334 Ubiquitination
R338 Methylation
S339 Phosphorylation
S341 Phosphorylation
S344 Phosphorylation
S347 Phosphorylation
S357 Phosphorylation
T361 Phosphorylation
S362 Phosphorylation
Y364 Phosphorylation
S369 Phosphorylation
S411 Phosphorylation
S415 Phosphorylation
S426 Phosphorylation
T439 Phosphorylation
K441 Acetylation
S457 Phosphorylation
T458 Phosphorylation
T462 Phosphorylation
S478 Phosphorylation
K491 Methylation
K491 Ubiquitination
T492 Phosphorylation
S494 Phosphorylation
T495 Phosphorylation
S496 Phosphorylation
S506 Phosphorylation
K510 Ubiquitination
T511 Phosphorylation
S521 Phosphorylation
S527 Phosphorylation
S529 Phosphorylation
S532 Phosphorylation
S534 Phosphorylation
S546 Phosphorylation
S548 Phosphorylation
T550 Phosphorylation
S558 Phosphorylation
T560 Phosphorylation
S562 Phosphorylation
T564 Phosphorylation
S565 Phosphorylation
S568 Phosphorylation
K570 Ubiquitination
T582 Phosphorylation

Research Backgrounds

Function:

Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein. Nucleus membrane>Single-pass membrane protein. Cell junction>Tight junction. Nucleus>Nucleolus. Cytoplasm>Perinuclear region.
Note: In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.

Subunit Structure:

Interacts with BCCIP, CREBBP/CBP and RELA/p65.

Restrictive clause

 

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