AGT Antibody - #BF0069
| Product: | AGT Antibody |
| Catalog: | BF0069 |
| Description: | Mouse monoclonal antibody to AGT |
| Application: | WB ELISA |
| Reactivity: | Human |
| Mol.Wt.: | 53kDa; 22kD(Calculated). |
| Uniprot: | P16455 |
| RRID: | AB_2833719 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# BF0069, RRID:AB_2833719.
Fold/Unfold
6 O methylguanine DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase; Agat; AGT; AI267024; EC 2.1.1.63; Methylated DNA protein cysteine methyltransferase; Methylated-DNA--protein-cysteine methyltransferase; Methylguanine DNA methyltransferase; MGC107020; MGMT; MGMT_HUMAN; O 6 methylguanine DNA alkyltransferase; O 6 methylguanine DNA methyltransferase; O-6-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase;
Immunogens
Purified recombinant fragment of human AGT expressed in E. Coli.
- P16455 MGMT_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVEVPAPAAVLGGPEPLMQCTAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKVVKFGEVISYQQLAALAGNPKAARAVGGAMRGNPVPILIPCHRVVCSSGAVGNYSGGLAVKEWLLAHEGHRLGKPGLGGSSGLAGAWLKGAGATSGSPPAGRN
PTMs - P16455 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K8 | Ubiquitination | Uniprot | |
| T10 | Phosphorylation | Uniprot | |
| T11 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| K104 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| Y158 | Phosphorylation | Uniprot | |
| K165 | Acetylation | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| S201 | Phosphorylation | Uniprot |
Research Backgrounds
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Nucleus.
Belongs to the MGMT family.
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