CD20 Antibody - #BF0233

Product: CD20 Antibody
Catalog: BF0233
Description: Mouse monoclonal antibody to CD20
Application: IHC IF/ICC ELISA
Reactivity: Human
Mol.Wt.: 33kDa.; 33kD(Calculated).
Uniprot: P11836
RRID: AB_2833614

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
ELISA 1:10000, IHC 1:200-1:1000, IF/ICC 1:200-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFB1589]
Specificity:
CD20 antibody detects endogenous levels of total CD20.
RRID:
AB_2833614
Cite Format: Affinity Biosciences Cat# BF0233, RRID:AB_2833614.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

APY; ATOPY; B lymphocyte antigen CD20; B Lymphocyte Cell Surface Antigen B1; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; B1; Bp 35; Bp35; CD 20; CD20; CD20 antigen; CD20 receptor; CD20_HUMAN; CVID5; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU 16; LEU16; leukocyte surface antigen Leu 16; Leukocyte surface antigen Leu-16; Ly44; Membrane spanning 4 domains A1; Membrane spanning 4 domains subfamily A member 2; Membrane-spanning 4-domains subfamily A member 1; MS4A1; MS4A2; S7;

Immunogens

Immunogen:

Purified recombinant fragment of human CD20 expressed in E. Coli.

Uniprot:

P11836(CD20_HUMAN) >>Visit HPA database.

Gene(ID):
MS4A1(931)
Expression:
P11836 CD20_HUMAN:

Expressed on B-cells.

Description:
CD20 (MS4A1, membrane-spanning 4-domains, subfamily A, member 1) is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit. And it is known to accelerate the G0 to G1 progression induced by IGF-1.
Sequence:
MTTPRNSVNGTFPAEPMKGPIAMQSGPKPLFRRMSSLVGPTQSFFMRESKTLGAVQIMNGLFHIALGGLLMIPAGIYAPICVTVWYPLWGGIMYIISGSLLAATEKNSRKCLVKGKMIMNSLSLFAAISGMILSIMDILNIKISHFLKMESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIFAFFQELVIAGIVENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSPIENDSSP

PTMs - P11836 As Substrate

Site PTM Type Enzyme
T2 Phosphorylation
T3 Phosphorylation
S7 Phosphorylation
T11 Phosphorylation
S25 Phosphorylation
S35 Phosphorylation
S36 Phosphorylation
T41 Phosphorylation
S121 Phosphorylation
S225 Phosphorylation
S231 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
K235 Ubiquitination
K236 Ubiquitination
K243 Ubiquitination
T250 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
S253 Phosphorylation
S254 Phosphorylation
S289 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)

Research Backgrounds

Function:

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR.

PTMs:

Phosphorylated on serines and threonines in resting B-cells. Protein kinase C/PKC can use CD20 as substrate.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cell membrane>Lipid-anchor.
Note: Constitutively associated with membrane rafts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed on B-cells.

Subunit Structure:

Forms homotetramers. Interacts with the heavy and light chains of cell surface IgM, the antigen-binding components of the BCR.

Family&Domains:

Belongs to the MS4A family.

Research Fields

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

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